Enhanced Muscle Strength in Dyslipidemic Mice and Its Relation to Increased Capacity for Fatty Acid Oxidation

Dyslipidemia is commonly linked to skeletal muscle dysfunction, accumulation of intramyocellular lipids, and insulin resistance. However, our previous research indicated that dyslipidemia in apolipoprotein E and low-density lipoprotein receptor double knock-out mice (ApoE/LDLR -/-) leads to improvem...

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Autores principales:	Marta Tomczyk, Alicja Braczko, Patrycja Jablonska, Adriana Mika, Kamil Przyborowski, Agata Jedrzejewska, Oliwia Krol, Filip Kus, Tomasz Sledzinski, Stefan Chlopicki, Ewa M. Slominska, Ryszard T. Smolenski
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	skeletal muscle metabolic disorders dyslipidemia mitochondria Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/0b28bbcba6ba443abf9519a38c17710c
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spelling	oai:doaj.org-article:0b28bbcba6ba443abf9519a38c17710c2021-11-25T17:54:46ZEnhanced Muscle Strength in Dyslipidemic Mice and Its Relation to Increased Capacity for Fatty Acid Oxidation10.3390/ijms2222122511422-00671661-6596https://doaj.org/article/0b28bbcba6ba443abf9519a38c17710c2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12251https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Dyslipidemia is commonly linked to skeletal muscle dysfunction, accumulation of intramyocellular lipids, and insulin resistance. However, our previous research indicated that dyslipidemia in apolipoprotein E and low-density lipoprotein receptor double knock-out mice (ApoE/LDLR -/-) leads to improvement of exercise capacity. This study aimed to investigate in detail skeletal muscle function and metabolism in these dyslipidemic mice. We found that ApoE/LDLR -/- mice showed an increased grip strength as well as increased troponins, and Mhc2 levels in skeletal muscle. It was accompanied by the increased skeletal muscle mitochondria numbers (judged by increased citrate synthase activity) and elevated total adenine nucleotides pool. We noted increased triglycerides contents in skeletal muscles and increased serum free fatty acids (FFA) levels in ApoE/LDLR -/- mice. Importantly, Ranolazine mediated inhibition of FFA oxidation in ApoE/LDLR -/- mice led to the reduction of exercise capacity and total adenine nucleotides pool. Thus, this study demonstrated that increased capacity for fatty acid oxidation, an adaptive response to dyslipidemia leads to improved cellular energetics that translates to increased skeletal muscle strength and contributes to increased exercise capacity in ApoE/LDLR -/- mice.Marta TomczykAlicja BraczkoPatrycja JablonskaAdriana MikaKamil PrzyborowskiAgata JedrzejewskaOliwia KrolFilip KusTomasz SledzinskiStefan ChlopickiEwa M. SlominskaRyszard T. SmolenskiMDPI AGarticleskeletal musclemetabolic disordersdyslipidemiamitochondriaBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12251, p 12251 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	skeletal muscle metabolic disorders dyslipidemia mitochondria Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	skeletal muscle metabolic disorders dyslipidemia mitochondria Biology (General) QH301-705.5 Chemistry QD1-999 Marta Tomczyk Alicja Braczko Patrycja Jablonska Adriana Mika Kamil Przyborowski Agata Jedrzejewska Oliwia Krol Filip Kus Tomasz Sledzinski Stefan Chlopicki Ewa M. Slominska Ryszard T. Smolenski Enhanced Muscle Strength in Dyslipidemic Mice and Its Relation to Increased Capacity for Fatty Acid Oxidation
description	Dyslipidemia is commonly linked to skeletal muscle dysfunction, accumulation of intramyocellular lipids, and insulin resistance. However, our previous research indicated that dyslipidemia in apolipoprotein E and low-density lipoprotein receptor double knock-out mice (ApoE/LDLR -/-) leads to improvement of exercise capacity. This study aimed to investigate in detail skeletal muscle function and metabolism in these dyslipidemic mice. We found that ApoE/LDLR -/- mice showed an increased grip strength as well as increased troponins, and Mhc2 levels in skeletal muscle. It was accompanied by the increased skeletal muscle mitochondria numbers (judged by increased citrate synthase activity) and elevated total adenine nucleotides pool. We noted increased triglycerides contents in skeletal muscles and increased serum free fatty acids (FFA) levels in ApoE/LDLR -/- mice. Importantly, Ranolazine mediated inhibition of FFA oxidation in ApoE/LDLR -/- mice led to the reduction of exercise capacity and total adenine nucleotides pool. Thus, this study demonstrated that increased capacity for fatty acid oxidation, an adaptive response to dyslipidemia leads to improved cellular energetics that translates to increased skeletal muscle strength and contributes to increased exercise capacity in ApoE/LDLR -/- mice.
format	article
author	Marta Tomczyk Alicja Braczko Patrycja Jablonska Adriana Mika Kamil Przyborowski Agata Jedrzejewska Oliwia Krol Filip Kus Tomasz Sledzinski Stefan Chlopicki Ewa M. Slominska Ryszard T. Smolenski
author_facet	Marta Tomczyk Alicja Braczko Patrycja Jablonska Adriana Mika Kamil Przyborowski Agata Jedrzejewska Oliwia Krol Filip Kus Tomasz Sledzinski Stefan Chlopicki Ewa M. Slominska Ryszard T. Smolenski
author_sort	Marta Tomczyk
title	Enhanced Muscle Strength in Dyslipidemic Mice and Its Relation to Increased Capacity for Fatty Acid Oxidation
title_short	Enhanced Muscle Strength in Dyslipidemic Mice and Its Relation to Increased Capacity for Fatty Acid Oxidation
title_full	Enhanced Muscle Strength in Dyslipidemic Mice and Its Relation to Increased Capacity for Fatty Acid Oxidation
title_fullStr	Enhanced Muscle Strength in Dyslipidemic Mice and Its Relation to Increased Capacity for Fatty Acid Oxidation
title_full_unstemmed	Enhanced Muscle Strength in Dyslipidemic Mice and Its Relation to Increased Capacity for Fatty Acid Oxidation
title_sort	enhanced muscle strength in dyslipidemic mice and its relation to increased capacity for fatty acid oxidation
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0b28bbcba6ba443abf9519a38c17710c
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Enhanced Muscle Strength in Dyslipidemic Mice and Its Relation to Increased Capacity for Fatty Acid Oxidation

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