Genetic biomarkers for ALS disease in transgenic SOD1(G93A) mice.

The pathophysiological mechanisms of both familial and sporadic Amyotrophic Lateral Sclerosis (ALS) are unknown, although growing evidence suggests that skeletal muscle tissue is a primary target of ALS toxicity. Skeletal muscle biopsies were performed on transgenic SOD1(G93A) mice, a mouse model of...

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Autores principales:	Ana C Calvo, Raquel Manzano, Gabriela Atencia-Cibreiro, Sara Oliván, María J Muñoz, Pilar Zaragoza, Pilar Cordero-Vázquez, Jesús Esteban-Pérez, Alberto García-Redondo, Rosario Osta
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0b29545086dd470c8e4430df219cdfb0
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spelling	oai:doaj.org-article:0b29545086dd470c8e4430df219cdfb02021-11-18T07:25:51ZGenetic biomarkers for ALS disease in transgenic SOD1(G93A) mice.1932-620310.1371/journal.pone.0032632https://doaj.org/article/0b29545086dd470c8e4430df219cdfb02012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22412900/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The pathophysiological mechanisms of both familial and sporadic Amyotrophic Lateral Sclerosis (ALS) are unknown, although growing evidence suggests that skeletal muscle tissue is a primary target of ALS toxicity. Skeletal muscle biopsies were performed on transgenic SOD1(G93A) mice, a mouse model of ALS, to determine genetic biomarkers of disease longevity. Mice were anesthetized with isoflurane, and three biopsy samples were obtained per animal at the three main stages of the disease. Transcriptional expression levels of seventeen genes, Ankrd1, Calm1, Col19a1, Fbxo32, Gsr, Impa1, Mef2c, Mt2, Myf5, Myod1, Myog, Nnt, Nogo A, Pax7, Rrad, Sln and Snx10, were tested in each muscle biopsy sample. Total RNA was extracted using TRIzol Reagent according to the manufacturer's protocol, and variations in gene expression were assayed by real-time PCR for all of the samples. The Pearson correlation coefficient was used to determine the linear correlation between transcriptional expression levels throughout disease progression and longevity. Consistent with the results obtained from total skeletal muscle of transgenic SOD1(G93A) mice and 74-day-old denervated mice, five genes (Mef2c, Gsr, Col19a1, Calm1 and Snx10) could be considered potential genetic biomarkers of longevity in transgenic SOD1(G93A) mice. These results are important because they may lead to the exploration of previously unexamined tissues in the search for new disease biomarkers and even to the application of these findings in human studies.Ana C CalvoRaquel ManzanoGabriela Atencia-CibreiroSara OlivánMaría J MuñozPilar ZaragozaPilar Cordero-VázquezJesús Esteban-PérezAlberto García-RedondoRosario OstaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 3, p e32632 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ana C Calvo Raquel Manzano Gabriela Atencia-Cibreiro Sara Oliván María J Muñoz Pilar Zaragoza Pilar Cordero-Vázquez Jesús Esteban-Pérez Alberto García-Redondo Rosario Osta Genetic biomarkers for ALS disease in transgenic SOD1(G93A) mice.
description	The pathophysiological mechanisms of both familial and sporadic Amyotrophic Lateral Sclerosis (ALS) are unknown, although growing evidence suggests that skeletal muscle tissue is a primary target of ALS toxicity. Skeletal muscle biopsies were performed on transgenic SOD1(G93A) mice, a mouse model of ALS, to determine genetic biomarkers of disease longevity. Mice were anesthetized with isoflurane, and three biopsy samples were obtained per animal at the three main stages of the disease. Transcriptional expression levels of seventeen genes, Ankrd1, Calm1, Col19a1, Fbxo32, Gsr, Impa1, Mef2c, Mt2, Myf5, Myod1, Myog, Nnt, Nogo A, Pax7, Rrad, Sln and Snx10, were tested in each muscle biopsy sample. Total RNA was extracted using TRIzol Reagent according to the manufacturer's protocol, and variations in gene expression were assayed by real-time PCR for all of the samples. The Pearson correlation coefficient was used to determine the linear correlation between transcriptional expression levels throughout disease progression and longevity. Consistent with the results obtained from total skeletal muscle of transgenic SOD1(G93A) mice and 74-day-old denervated mice, five genes (Mef2c, Gsr, Col19a1, Calm1 and Snx10) could be considered potential genetic biomarkers of longevity in transgenic SOD1(G93A) mice. These results are important because they may lead to the exploration of previously unexamined tissues in the search for new disease biomarkers and even to the application of these findings in human studies.
format	article
author	Ana C Calvo Raquel Manzano Gabriela Atencia-Cibreiro Sara Oliván María J Muñoz Pilar Zaragoza Pilar Cordero-Vázquez Jesús Esteban-Pérez Alberto García-Redondo Rosario Osta
author_facet	Ana C Calvo Raquel Manzano Gabriela Atencia-Cibreiro Sara Oliván María J Muñoz Pilar Zaragoza Pilar Cordero-Vázquez Jesús Esteban-Pérez Alberto García-Redondo Rosario Osta
author_sort	Ana C Calvo
title	Genetic biomarkers for ALS disease in transgenic SOD1(G93A) mice.
title_short	Genetic biomarkers for ALS disease in transgenic SOD1(G93A) mice.
title_full	Genetic biomarkers for ALS disease in transgenic SOD1(G93A) mice.
title_fullStr	Genetic biomarkers for ALS disease in transgenic SOD1(G93A) mice.
title_full_unstemmed	Genetic biomarkers for ALS disease in transgenic SOD1(G93A) mice.
title_sort	genetic biomarkers for als disease in transgenic sod1(g93a) mice.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/0b29545086dd470c8e4430df219cdfb0
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Genetic biomarkers for ALS disease in transgenic SOD1(G93A) mice.

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