Microarray-based approach identifies differentially expressed microRNAs in porcine sexually immature and mature testes.

<h4>Background</h4>MicroRNAs (miRNAs) are short non-coding RNA molecules which are proved to be involved in mammalian spermatogenesis. Their expression and function in the porcine germ cells are not fully understood.<h4>Methodology</h4>We employed a miRNA microarray containin...

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Autores principales:	Lifan Luo, Lianzhi Ye, Gang Liu, Guochao Shao, Rong Zheng, Zhuqing Ren, Bo Zuo, Dequan Xu, Minggang Lei, Siwen Jiang, Changyan Deng, Yuanzhu Xiong, Fenge Li
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0b3ed3c7063541db9450cb5e21df9cef
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spelling	oai:doaj.org-article:0b3ed3c7063541db9450cb5e21df9cef2021-11-18T06:35:56ZMicroarray-based approach identifies differentially expressed microRNAs in porcine sexually immature and mature testes.1932-620310.1371/journal.pone.0011744https://doaj.org/article/0b3ed3c7063541db9450cb5e21df9cef2010-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20805883/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>MicroRNAs (miRNAs) are short non-coding RNA molecules which are proved to be involved in mammalian spermatogenesis. Their expression and function in the porcine germ cells are not fully understood.<h4>Methodology</h4>We employed a miRNA microarray containing 1260 unique miRNA probes to evaluate the miRNA expression patterns between sexually immature (60-day) and mature (180-day) pig testes. One hundred and twenty nine miRNAs representing 164 reporter miRNAs were expressed differently (p<0.1). Fifty one miRNAs were significantly up-regulated and 78 miRNAs were down-regulated in mature testes. Nine of these differentially expressed miRNAs were validated using quantitative RT-PCR assay. Totally 15,919 putative miRNA-target sites were detected by using RNA22 method to align 445 NCBI pig cDNA sequences with these 129 differentially expressed miRNAs, and seven putative target genes involved in spermatogenesis including DAZL, RNF4 gene were simply confirmed by quantitative RT-PCR.<h4>Conclusions</h4>Overall, the results of this study indicated specific miRNAs expression in porcine testes and suggested that miRNAs had a role in regulating spermatogenesis.Lifan LuoLianzhi YeGang LiuGuochao ShaoRong ZhengZhuqing RenBo ZuoDequan XuMinggang LeiSiwen JiangChangyan DengYuanzhu XiongFenge LiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 8, p e11744 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lifan Luo Lianzhi Ye Gang Liu Guochao Shao Rong Zheng Zhuqing Ren Bo Zuo Dequan Xu Minggang Lei Siwen Jiang Changyan Deng Yuanzhu Xiong Fenge Li Microarray-based approach identifies differentially expressed microRNAs in porcine sexually immature and mature testes.
description	<h4>Background</h4>MicroRNAs (miRNAs) are short non-coding RNA molecules which are proved to be involved in mammalian spermatogenesis. Their expression and function in the porcine germ cells are not fully understood.<h4>Methodology</h4>We employed a miRNA microarray containing 1260 unique miRNA probes to evaluate the miRNA expression patterns between sexually immature (60-day) and mature (180-day) pig testes. One hundred and twenty nine miRNAs representing 164 reporter miRNAs were expressed differently (p<0.1). Fifty one miRNAs were significantly up-regulated and 78 miRNAs were down-regulated in mature testes. Nine of these differentially expressed miRNAs were validated using quantitative RT-PCR assay. Totally 15,919 putative miRNA-target sites were detected by using RNA22 method to align 445 NCBI pig cDNA sequences with these 129 differentially expressed miRNAs, and seven putative target genes involved in spermatogenesis including DAZL, RNF4 gene were simply confirmed by quantitative RT-PCR.<h4>Conclusions</h4>Overall, the results of this study indicated specific miRNAs expression in porcine testes and suggested that miRNAs had a role in regulating spermatogenesis.
format	article
author	Lifan Luo Lianzhi Ye Gang Liu Guochao Shao Rong Zheng Zhuqing Ren Bo Zuo Dequan Xu Minggang Lei Siwen Jiang Changyan Deng Yuanzhu Xiong Fenge Li
author_facet	Lifan Luo Lianzhi Ye Gang Liu Guochao Shao Rong Zheng Zhuqing Ren Bo Zuo Dequan Xu Minggang Lei Siwen Jiang Changyan Deng Yuanzhu Xiong Fenge Li
author_sort	Lifan Luo
title	Microarray-based approach identifies differentially expressed microRNAs in porcine sexually immature and mature testes.
title_short	Microarray-based approach identifies differentially expressed microRNAs in porcine sexually immature and mature testes.
title_full	Microarray-based approach identifies differentially expressed microRNAs in porcine sexually immature and mature testes.
title_fullStr	Microarray-based approach identifies differentially expressed microRNAs in porcine sexually immature and mature testes.
title_full_unstemmed	Microarray-based approach identifies differentially expressed microRNAs in porcine sexually immature and mature testes.
title_sort	microarray-based approach identifies differentially expressed micrornas in porcine sexually immature and mature testes.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/0b3ed3c7063541db9450cb5e21df9cef
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Microarray-based approach identifies differentially expressed microRNAs in porcine sexually immature and mature testes.

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