Functional profiling of single CRISPR/Cas9-edited human long-term hematopoietic stem cells

Previous gene editing in haematopoietic stem cells (HSCs) has focussed on a heterogeneous CD34+ population. Here, the authors demonstrate high efficiency CRISPR/Cas9-based editing of purified long-term HSCs using non-homologous end joining and homology-directed repair, by directing isoform-specific...

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Autores principales:	Elvin Wagenblast, Maria Azkanaz, Sabrina A. Smith, Lorien Shakib, Jessica L. McLeod, Gabriela Krivdova, Joana Araújo, Leonard D. Shultz, Olga I. Gan, John E. Dick, Eric R. Lechman
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/0b4499f298ce4f27a378fb08edca0925
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spelling	oai:doaj.org-article:0b4499f298ce4f27a378fb08edca09252021-12-02T15:36:06ZFunctional profiling of single CRISPR/Cas9-edited human long-term hematopoietic stem cells10.1038/s41467-019-12726-02041-1723https://doaj.org/article/0b4499f298ce4f27a378fb08edca09252019-10-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-12726-0https://doaj.org/toc/2041-1723Previous gene editing in haematopoietic stem cells (HSCs) has focussed on a heterogeneous CD34+ population. Here, the authors demonstrate high efficiency CRISPR/Cas9-based editing of purified long-term HSCs using non-homologous end joining and homology-directed repair, by directing isoform-specific expression of GATA1.Elvin WagenblastMaria AzkanazSabrina A. SmithLorien ShakibJessica L. McLeodGabriela KrivdovaJoana AraújoLeonard D. ShultzOlga I. GanJohn E. DickEric R. LechmanNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Elvin Wagenblast Maria Azkanaz Sabrina A. Smith Lorien Shakib Jessica L. McLeod Gabriela Krivdova Joana Araújo Leonard D. Shultz Olga I. Gan John E. Dick Eric R. Lechman Functional profiling of single CRISPR/Cas9-edited human long-term hematopoietic stem cells
description	Previous gene editing in haematopoietic stem cells (HSCs) has focussed on a heterogeneous CD34+ population. Here, the authors demonstrate high efficiency CRISPR/Cas9-based editing of purified long-term HSCs using non-homologous end joining and homology-directed repair, by directing isoform-specific expression of GATA1.
format	article
author	Elvin Wagenblast Maria Azkanaz Sabrina A. Smith Lorien Shakib Jessica L. McLeod Gabriela Krivdova Joana Araújo Leonard D. Shultz Olga I. Gan John E. Dick Eric R. Lechman
author_facet	Elvin Wagenblast Maria Azkanaz Sabrina A. Smith Lorien Shakib Jessica L. McLeod Gabriela Krivdova Joana Araújo Leonard D. Shultz Olga I. Gan John E. Dick Eric R. Lechman
author_sort	Elvin Wagenblast
title	Functional profiling of single CRISPR/Cas9-edited human long-term hematopoietic stem cells
title_short	Functional profiling of single CRISPR/Cas9-edited human long-term hematopoietic stem cells
title_full	Functional profiling of single CRISPR/Cas9-edited human long-term hematopoietic stem cells
title_fullStr	Functional profiling of single CRISPR/Cas9-edited human long-term hematopoietic stem cells
title_full_unstemmed	Functional profiling of single CRISPR/Cas9-edited human long-term hematopoietic stem cells
title_sort	functional profiling of single crispr/cas9-edited human long-term hematopoietic stem cells
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/0b4499f298ce4f27a378fb08edca0925
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Functional profiling of single CRISPR/Cas9-edited human long-term hematopoietic stem cells

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