Isothermal Recombinase Polymerase Amplification (RPA) of <i>E. coli</i> gDNA in Commercially Fabricated PCB-Based Microfluidic Platforms

Printed circuit board (PCB) technology has been recently proposed as a convenient platform for seamlessly integrating electronics and microfluidics in the same substrate, thus facilitating the introduction of integrated and low-cost microfluidic devices to the market, thanks to the inherent upscalin...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Maria Georgoutsou-Spyridonos, Myrto Filippidou, Georgia D. Kaprou, Dimitrios C. Mastellos, Stavros Chatzandroulis, Angeliki Tserepi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	PCB technology DNA amplification RPA microfluidics microheaters <i>E. coli</i> Mechanical engineering and machinery TJ1-1570
Acceso en línea:	https://doaj.org/article/0b499f969b6b4948a3af0fa25c02e8ab
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0b499f969b6b4948a3af0fa25c02e8ab
record_format	dspace
spelling	oai:doaj.org-article:0b499f969b6b4948a3af0fa25c02e8ab2021-11-25T18:23:35ZIsothermal Recombinase Polymerase Amplification (RPA) of <i>E. coli</i> gDNA in Commercially Fabricated PCB-Based Microfluidic Platforms10.3390/mi121113872072-666Xhttps://doaj.org/article/0b499f969b6b4948a3af0fa25c02e8ab2021-11-01T00:00:00Zhttps://www.mdpi.com/2072-666X/12/11/1387https://doaj.org/toc/2072-666XPrinted circuit board (PCB) technology has been recently proposed as a convenient platform for seamlessly integrating electronics and microfluidics in the same substrate, thus facilitating the introduction of integrated and low-cost microfluidic devices to the market, thanks to the inherent upscaling potential of the PCB industry. Herein, a microfluidic chip, encompassing on PCB both a meandering microchannel and microheaters to accommodate recombinase polymerase amplification (RPA), is designed and commercially fabricated for the first time on PCB. The developed microchip is validated for RPA-based amplification of two <i>E. coli</i> target genes compared to a conventional thermocycler. The RPA performance of the PCB microchip was found to be well-comparable to that of a thermocycler yet with a remarkably lower power consumption (0.6 W). This microchip is intended for seamless integration with biosensors in the same PCB substrate for the development of a point-of-care (POC) molecular diagnostics platform.Maria Georgoutsou-SpyridonosMyrto FilippidouGeorgia D. KaprouDimitrios C. MastellosStavros ChatzandroulisAngeliki TserepiMDPI AGarticlePCB technologyDNA amplificationRPAmicrofluidicsmicroheaters<i>E. coli</i>Mechanical engineering and machineryTJ1-1570ENMicromachines, Vol 12, Iss 1387, p 1387 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	PCB technology DNA amplification RPA microfluidics microheaters <i>E. coli</i> Mechanical engineering and machinery TJ1-1570
spellingShingle	PCB technology DNA amplification RPA microfluidics microheaters <i>E. coli</i> Mechanical engineering and machinery TJ1-1570 Maria Georgoutsou-Spyridonos Myrto Filippidou Georgia D. Kaprou Dimitrios C. Mastellos Stavros Chatzandroulis Angeliki Tserepi Isothermal Recombinase Polymerase Amplification (RPA) of <i>E. coli</i> gDNA in Commercially Fabricated PCB-Based Microfluidic Platforms
description	Printed circuit board (PCB) technology has been recently proposed as a convenient platform for seamlessly integrating electronics and microfluidics in the same substrate, thus facilitating the introduction of integrated and low-cost microfluidic devices to the market, thanks to the inherent upscaling potential of the PCB industry. Herein, a microfluidic chip, encompassing on PCB both a meandering microchannel and microheaters to accommodate recombinase polymerase amplification (RPA), is designed and commercially fabricated for the first time on PCB. The developed microchip is validated for RPA-based amplification of two <i>E. coli</i> target genes compared to a conventional thermocycler. The RPA performance of the PCB microchip was found to be well-comparable to that of a thermocycler yet with a remarkably lower power consumption (0.6 W). This microchip is intended for seamless integration with biosensors in the same PCB substrate for the development of a point-of-care (POC) molecular diagnostics platform.
format	article
author	Maria Georgoutsou-Spyridonos Myrto Filippidou Georgia D. Kaprou Dimitrios C. Mastellos Stavros Chatzandroulis Angeliki Tserepi
author_facet	Maria Georgoutsou-Spyridonos Myrto Filippidou Georgia D. Kaprou Dimitrios C. Mastellos Stavros Chatzandroulis Angeliki Tserepi
author_sort	Maria Georgoutsou-Spyridonos
title	Isothermal Recombinase Polymerase Amplification (RPA) of <i>E. coli</i> gDNA in Commercially Fabricated PCB-Based Microfluidic Platforms
title_short	Isothermal Recombinase Polymerase Amplification (RPA) of <i>E. coli</i> gDNA in Commercially Fabricated PCB-Based Microfluidic Platforms
title_full	Isothermal Recombinase Polymerase Amplification (RPA) of <i>E. coli</i> gDNA in Commercially Fabricated PCB-Based Microfluidic Platforms
title_fullStr	Isothermal Recombinase Polymerase Amplification (RPA) of <i>E. coli</i> gDNA in Commercially Fabricated PCB-Based Microfluidic Platforms
title_full_unstemmed	Isothermal Recombinase Polymerase Amplification (RPA) of <i>E. coli</i> gDNA in Commercially Fabricated PCB-Based Microfluidic Platforms
title_sort	isothermal recombinase polymerase amplification (rpa) of <i>e. coli</i> gdna in commercially fabricated pcb-based microfluidic platforms
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0b499f969b6b4948a3af0fa25c02e8ab
work_keys_str_mv	AT mariageorgoutsouspyridonos isothermalrecombinasepolymeraseamplificationrpaofiecoliigdnaincommerciallyfabricatedpcbbasedmicrofluidicplatforms AT myrtofilippidou isothermalrecombinasepolymeraseamplificationrpaofiecoliigdnaincommerciallyfabricatedpcbbasedmicrofluidicplatforms AT georgiadkaprou isothermalrecombinasepolymeraseamplificationrpaofiecoliigdnaincommerciallyfabricatedpcbbasedmicrofluidicplatforms AT dimitrioscmastellos isothermalrecombinasepolymeraseamplificationrpaofiecoliigdnaincommerciallyfabricatedpcbbasedmicrofluidicplatforms AT stavroschatzandroulis isothermalrecombinasepolymeraseamplificationrpaofiecoliigdnaincommerciallyfabricatedpcbbasedmicrofluidicplatforms AT angelikitserepi isothermalrecombinasepolymeraseamplificationrpaofiecoliigdnaincommerciallyfabricatedpcbbasedmicrofluidicplatforms
_version_	1718411178499112960

Isothermal Recombinase Polymerase Amplification (RPA) of <i>E. coli</i> gDNA in Commercially Fabricated PCB-Based Microfluidic Platforms

Ejemplares similares