Action of lytic polysaccharide monooxygenase on plant tissue is governed by cellular type
Abstract Lignocellulosic biomass bioconversion is hampered by the structural and chemical complexity of the network created by cellulose, hemicellulose and lignin. Biological conversion of lignocellulose involves synergistic action of a large array of enzymes including the recently discovered lytic...
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Nature Portfolio
2017
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oai:doaj.org-article:0b52ea60861b40d3b40b5983a849252b2021-12-02T15:05:40ZAction of lytic polysaccharide monooxygenase on plant tissue is governed by cellular type10.1038/s41598-017-17938-22045-2322https://doaj.org/article/0b52ea60861b40d3b40b5983a849252b2017-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-17938-2https://doaj.org/toc/2045-2322Abstract Lignocellulosic biomass bioconversion is hampered by the structural and chemical complexity of the network created by cellulose, hemicellulose and lignin. Biological conversion of lignocellulose involves synergistic action of a large array of enzymes including the recently discovered lytic polysaccharide monooxygenases (LPMOs) that perform oxidative cleavage of cellulose. Using in situ imaging by synchrotron UV fluorescence, we have shown that the addition of AA9 LPMO (from Podospora anserina) to cellulases cocktail improves the progression of enzymes in delignified Miscanthus x giganteus as observed at tissular levels. In situ chemical monitoring of cell wall modifications performed by synchrotron infrared spectroscopy during enzymatic hydrolysis demonstrated that the boosting effect of the AA9 LPMO was dependent on the cellular type indicating contrasted recalcitrance levels in plant tissues. Our study provides a useful strategy for investigating enzyme dynamics and activity in plant cell wall to improve enzymatic cocktails aimed at expanding lignocelluloses biorefinery.Brigitte ChabbertAnouck HabrantMickaël HerbautLaurence FoulonVéronique Aguié-BéghinSona GarajovaSacha GriselChloé Bennati-GranierIsabelle Gimbert-HerpoëlFrédéric JammeMatthieu RéfrégiersChristophe SandtJean-Guy BerrinGabriel PaësNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017) |
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DOAJ |
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Medicine R Science Q Brigitte Chabbert Anouck Habrant Mickaël Herbaut Laurence Foulon Véronique Aguié-Béghin Sona Garajova Sacha Grisel Chloé Bennati-Granier Isabelle Gimbert-Herpoël Frédéric Jamme Matthieu Réfrégiers Christophe Sandt Jean-Guy Berrin Gabriel Paës Action of lytic polysaccharide monooxygenase on plant tissue is governed by cellular type |
| description |
Abstract Lignocellulosic biomass bioconversion is hampered by the structural and chemical complexity of the network created by cellulose, hemicellulose and lignin. Biological conversion of lignocellulose involves synergistic action of a large array of enzymes including the recently discovered lytic polysaccharide monooxygenases (LPMOs) that perform oxidative cleavage of cellulose. Using in situ imaging by synchrotron UV fluorescence, we have shown that the addition of AA9 LPMO (from Podospora anserina) to cellulases cocktail improves the progression of enzymes in delignified Miscanthus x giganteus as observed at tissular levels. In situ chemical monitoring of cell wall modifications performed by synchrotron infrared spectroscopy during enzymatic hydrolysis demonstrated that the boosting effect of the AA9 LPMO was dependent on the cellular type indicating contrasted recalcitrance levels in plant tissues. Our study provides a useful strategy for investigating enzyme dynamics and activity in plant cell wall to improve enzymatic cocktails aimed at expanding lignocelluloses biorefinery. |
| format |
article |
| author |
Brigitte Chabbert Anouck Habrant Mickaël Herbaut Laurence Foulon Véronique Aguié-Béghin Sona Garajova Sacha Grisel Chloé Bennati-Granier Isabelle Gimbert-Herpoël Frédéric Jamme Matthieu Réfrégiers Christophe Sandt Jean-Guy Berrin Gabriel Paës |
| author_facet |
Brigitte Chabbert Anouck Habrant Mickaël Herbaut Laurence Foulon Véronique Aguié-Béghin Sona Garajova Sacha Grisel Chloé Bennati-Granier Isabelle Gimbert-Herpoël Frédéric Jamme Matthieu Réfrégiers Christophe Sandt Jean-Guy Berrin Gabriel Paës |
| author_sort |
Brigitte Chabbert |
| title |
Action of lytic polysaccharide monooxygenase on plant tissue is governed by cellular type |
| title_short |
Action of lytic polysaccharide monooxygenase on plant tissue is governed by cellular type |
| title_full |
Action of lytic polysaccharide monooxygenase on plant tissue is governed by cellular type |
| title_fullStr |
Action of lytic polysaccharide monooxygenase on plant tissue is governed by cellular type |
| title_full_unstemmed |
Action of lytic polysaccharide monooxygenase on plant tissue is governed by cellular type |
| title_sort |
action of lytic polysaccharide monooxygenase on plant tissue is governed by cellular type |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/0b52ea60861b40d3b40b5983a849252b |
| work_keys_str_mv |
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