Blue light excited retinal intercepts cellular signaling

Abstract Photoreceptor chromophore, 11-cis retinal (11CR) and the photoproduct, all-trans retinal (ATR), are present in the retina at higher concentrations and interact with the visual cells. Non-visual cells in the body are also exposed to retinal that enters the circulation. Although the cornea an...

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Autores principales:	Kasun Ratnayake, John L. Payton, O. Harshana Lakmal, Ajith Karunarathne
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0b5736397292423cb8dd21d5b2c64427
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spelling	oai:doaj.org-article:0b5736397292423cb8dd21d5b2c644272021-12-02T15:09:06ZBlue light excited retinal intercepts cellular signaling10.1038/s41598-018-28254-82045-2322https://doaj.org/article/0b5736397292423cb8dd21d5b2c644272018-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-28254-8https://doaj.org/toc/2045-2322Abstract Photoreceptor chromophore, 11-cis retinal (11CR) and the photoproduct, all-trans retinal (ATR), are present in the retina at higher concentrations and interact with the visual cells. Non-visual cells in the body are also exposed to retinal that enters the circulation. Although the cornea and the lens of the eye are transparent to the blue light region where retinal can absorb and undergo excitation, the reported phototoxicity in the eye has been assigned to lipophilic non-degradable materials known as lipofuscins, which also includes retinal condensation products. The possibility of blue light excited retinal interacting with cells; intercepting signaling in the presence or absence of light has not been explored. Using live cell imaging and optogenetic signaling control, we uncovered that blue light-excited ATR and 11CR irreversibly change/distort plasma membrane (PM) bound phospholipid; phosphatidylinositol 4,5 bisphosphate (PIP2) and disrupt its function. This distortion in PIP2 was independent of visual or non-visual G-protein coupled receptor activation. The change in PIP2 was followed by an increase in the cytosolic calcium, excessive cell shape change, and cell death. Blue light alone or retinal alone did not perturb PIP2 or elicit cytosolic calcium increase. Our data also suggest that photoexcited retinal-induced PIP2 distortion and subsequent oxidative damage incur in the core of the PM. These findings suggest that retinal exerts light sensitivity to both photoreceptor and non-photoreceptor cells, and intercepts crucial signaling events, altering the cellular fate.Kasun RatnayakeJohn L. PaytonO. Harshana LakmalAjith KarunarathneNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-16 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kasun Ratnayake John L. Payton O. Harshana Lakmal Ajith Karunarathne Blue light excited retinal intercepts cellular signaling
description	Abstract Photoreceptor chromophore, 11-cis retinal (11CR) and the photoproduct, all-trans retinal (ATR), are present in the retina at higher concentrations and interact with the visual cells. Non-visual cells in the body are also exposed to retinal that enters the circulation. Although the cornea and the lens of the eye are transparent to the blue light region where retinal can absorb and undergo excitation, the reported phototoxicity in the eye has been assigned to lipophilic non-degradable materials known as lipofuscins, which also includes retinal condensation products. The possibility of blue light excited retinal interacting with cells; intercepting signaling in the presence or absence of light has not been explored. Using live cell imaging and optogenetic signaling control, we uncovered that blue light-excited ATR and 11CR irreversibly change/distort plasma membrane (PM) bound phospholipid; phosphatidylinositol 4,5 bisphosphate (PIP2) and disrupt its function. This distortion in PIP2 was independent of visual or non-visual G-protein coupled receptor activation. The change in PIP2 was followed by an increase in the cytosolic calcium, excessive cell shape change, and cell death. Blue light alone or retinal alone did not perturb PIP2 or elicit cytosolic calcium increase. Our data also suggest that photoexcited retinal-induced PIP2 distortion and subsequent oxidative damage incur in the core of the PM. These findings suggest that retinal exerts light sensitivity to both photoreceptor and non-photoreceptor cells, and intercepts crucial signaling events, altering the cellular fate.
format	article
author	Kasun Ratnayake John L. Payton O. Harshana Lakmal Ajith Karunarathne
author_facet	Kasun Ratnayake John L. Payton O. Harshana Lakmal Ajith Karunarathne
author_sort	Kasun Ratnayake
title	Blue light excited retinal intercepts cellular signaling
title_short	Blue light excited retinal intercepts cellular signaling
title_full	Blue light excited retinal intercepts cellular signaling
title_fullStr	Blue light excited retinal intercepts cellular signaling
title_full_unstemmed	Blue light excited retinal intercepts cellular signaling
title_sort	blue light excited retinal intercepts cellular signaling
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/0b5736397292423cb8dd21d5b2c64427
work_keys_str_mv	AT kasunratnayake bluelightexcitedretinalinterceptscellularsignaling AT johnlpayton bluelightexcitedretinalinterceptscellularsignaling AT oharshanalakmal bluelightexcitedretinalinterceptscellularsignaling AT ajithkarunarathne bluelightexcitedretinalinterceptscellularsignaling
_version_	1718387918565801984

Blue light excited retinal intercepts cellular signaling

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