Membrane properties of striatal direct and indirect pathway neurons in mouse and rat slices and their modulation by dopamine.

D1 and D2 receptor expressing striatal medium spiny neurons (MSNs) are ascribed to striatonigral ("direct") and striatopallidal ("indirect") pathways, respectively, that are believed to function antagonistically in motor control. Glutamatergic synaptic transmission onto the two t...

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Autores principales:	Henrike Planert, Thomas K Berger, Gilad Silberberg
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0b58f4812b234a709508bf0048ab8736
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spelling	oai:doaj.org-article:0b58f4812b234a709508bf0048ab87362021-11-18T07:55:15ZMembrane properties of striatal direct and indirect pathway neurons in mouse and rat slices and their modulation by dopamine.1932-620310.1371/journal.pone.0057054https://doaj.org/article/0b58f4812b234a709508bf0048ab87362013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23469183/?tool=EBIhttps://doaj.org/toc/1932-6203D1 and D2 receptor expressing striatal medium spiny neurons (MSNs) are ascribed to striatonigral ("direct") and striatopallidal ("indirect") pathways, respectively, that are believed to function antagonistically in motor control. Glutamatergic synaptic transmission onto the two types is differentially affected by Dopamine (DA), however, less is known about the effects on MSN intrinsic electrical properties. Using patch clamp recordings, we comprehensively characterized the two pathways in rats and mice, and investigated their DA modulation. We identified the direct pathway by retrograde labeling in rats, and in mice we used transgenic animals in which EGFP is expressed in D1 MSNs. MSNs were subjected to a series of current injections to pinpoint differences between the populations, and in mice also following bath application of DA. In both animal models, most electrical properties were similar, however, membrane excitability as measured by step and ramp current injections consistently differed, with direct pathway MSNs being less excitable than their counterparts. DA had opposite effects on excitability of D1 and D2 MSNs, counteracting the initial differences. Pronounced changes in AP shape were seen in D2 MSNs. In direct pathway MSNs, excitability increased across experimental conditions and parameters, and also when applying DA or the D1 agonist SKF-81297 in presence of blockers of cholinergic, GABAergic, and glutamatergic receptors. Thus, DA induced changes in excitability were D1 R mediated and intrinsic to direct pathway MSNs, and not a secondary network effect of altered synaptic transmission. DAergic modulation of intrinsic properties therefore acts in a synergistic manner with previously reported effects of DA on afferent synaptic transmission and dendritic processing, supporting the antagonistic model for direct vs. indirect striatal pathway function.Henrike PlanertThomas K BergerGilad SilberbergPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e57054 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Henrike Planert Thomas K Berger Gilad Silberberg Membrane properties of striatal direct and indirect pathway neurons in mouse and rat slices and their modulation by dopamine.
description	D1 and D2 receptor expressing striatal medium spiny neurons (MSNs) are ascribed to striatonigral ("direct") and striatopallidal ("indirect") pathways, respectively, that are believed to function antagonistically in motor control. Glutamatergic synaptic transmission onto the two types is differentially affected by Dopamine (DA), however, less is known about the effects on MSN intrinsic electrical properties. Using patch clamp recordings, we comprehensively characterized the two pathways in rats and mice, and investigated their DA modulation. We identified the direct pathway by retrograde labeling in rats, and in mice we used transgenic animals in which EGFP is expressed in D1 MSNs. MSNs were subjected to a series of current injections to pinpoint differences between the populations, and in mice also following bath application of DA. In both animal models, most electrical properties were similar, however, membrane excitability as measured by step and ramp current injections consistently differed, with direct pathway MSNs being less excitable than their counterparts. DA had opposite effects on excitability of D1 and D2 MSNs, counteracting the initial differences. Pronounced changes in AP shape were seen in D2 MSNs. In direct pathway MSNs, excitability increased across experimental conditions and parameters, and also when applying DA or the D1 agonist SKF-81297 in presence of blockers of cholinergic, GABAergic, and glutamatergic receptors. Thus, DA induced changes in excitability were D1 R mediated and intrinsic to direct pathway MSNs, and not a secondary network effect of altered synaptic transmission. DAergic modulation of intrinsic properties therefore acts in a synergistic manner with previously reported effects of DA on afferent synaptic transmission and dendritic processing, supporting the antagonistic model for direct vs. indirect striatal pathway function.
format	article
author	Henrike Planert Thomas K Berger Gilad Silberberg
author_facet	Henrike Planert Thomas K Berger Gilad Silberberg
author_sort	Henrike Planert
title	Membrane properties of striatal direct and indirect pathway neurons in mouse and rat slices and their modulation by dopamine.
title_short	Membrane properties of striatal direct and indirect pathway neurons in mouse and rat slices and their modulation by dopamine.
title_full	Membrane properties of striatal direct and indirect pathway neurons in mouse and rat slices and their modulation by dopamine.
title_fullStr	Membrane properties of striatal direct and indirect pathway neurons in mouse and rat slices and their modulation by dopamine.
title_full_unstemmed	Membrane properties of striatal direct and indirect pathway neurons in mouse and rat slices and their modulation by dopamine.
title_sort	membrane properties of striatal direct and indirect pathway neurons in mouse and rat slices and their modulation by dopamine.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/0b58f4812b234a709508bf0048ab8736
work_keys_str_mv	AT henrikeplanert membranepropertiesofstriataldirectandindirectpathwayneuronsinmouseandratslicesandtheirmodulationbydopamine AT thomaskberger membranepropertiesofstriataldirectandindirectpathwayneuronsinmouseandratslicesandtheirmodulationbydopamine AT giladsilberberg membranepropertiesofstriataldirectandindirectpathwayneuronsinmouseandratslicesandtheirmodulationbydopamine
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Membrane properties of striatal direct and indirect pathway neurons in mouse and rat slices and their modulation by dopamine.

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