Cordycepin suppresses glutamatergic and GABAergic synaptic transmission through activation of A1 adenosine receptor in rat hippocampal CA1 pyramidal neurons

Cordycepin (known as 3-deoxyadenosine, CRD), a natural product from the valuable traditional Chinese medicine Cordyceps militaris, has been reported to improve cognitive function and modulate neuroprotective effects on the central nervous system (CNS). However, the modulating mechanisms of cordycepi...

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Autores principales:	Jinxiu Wang, Yanchun Gong, Haoyuan Tan, Wenxi Li, Baiyi Yan, Chunfang Cheng, Juan Wan, Wei Sun, Chunhua Yuan, Li-Hua Yao
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Cordycepin Hippocampal slice Synaptic transmission Postsynaptic currents A1R antagonist Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/0b5a9b96dc954bf4875e65bfcb2d529c
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spelling	oai:doaj.org-article:0b5a9b96dc954bf4875e65bfcb2d529c2021-11-22T04:17:50ZCordycepin suppresses glutamatergic and GABAergic synaptic transmission through activation of A1 adenosine receptor in rat hippocampal CA1 pyramidal neurons0753-332210.1016/j.biopha.2021.112446https://doaj.org/article/0b5a9b96dc954bf4875e65bfcb2d529c2022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0753332221012324https://doaj.org/toc/0753-3322Cordycepin (known as 3-deoxyadenosine, CRD), a natural product from the valuable traditional Chinese medicine Cordyceps militaris, has been reported to improve cognitive function and modulate neuroprotective effects on the central nervous system (CNS). However, the modulating mechanisms of cordycepin on information processing in hippocampal CA1 pyramidal neurons are not fully understood. To clarify how cordycepin modulates synaptic responses of pyramidal neurons in rat hippocampal CA1 region, we conducted an electrophysiological experiment using whole-cell patch-clamp technique. The spontaneous and miniature excitatory postsynaptic currents (sEPSCs and mEPSCs, respectively) and the spontaneous and miniature inhibitory postsynaptic currents (sIPSCs and mIPSCs, respectively) recorded by this technique evaluated pure single or multi-synapse responses and enabled us to accurately quantify how cordycepin influenced the pre and postsynaptic aspects of synaptic transmission. The present results showed that cordycepin significantly decreased the frequency of both glutamatergic and GABAergic postsynaptic currents without affecting the amplitude, while these inhibitory effects were antagonized by the A1 adenosine receptor antagonist (DPCPX), but not the A2A (ZM 241385), A2B (MRS1754) and A3 (MRS1191) adenosine receptor antagonists. Taken together, our results suggested that cordycepin had a clear presynaptic effect on glutamatergic and GABAergic transmission, and provided novel evidence that cordycepin suppresses the synaptic transmission through the activation of A1AR.Jinxiu WangYanchun GongHaoyuan TanWenxi LiBaiyi YanChunfang ChengJuan WanWei SunChunhua YuanLi-Hua YaoElsevierarticleCordycepinHippocampal sliceSynaptic transmissionPostsynaptic currentsA1R antagonistTherapeutics. PharmacologyRM1-950ENBiomedicine & Pharmacotherapy, Vol 145, Iss , Pp 112446- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cordycepin Hippocampal slice Synaptic transmission Postsynaptic currents A1R antagonist Therapeutics. Pharmacology RM1-950
spellingShingle	Cordycepin Hippocampal slice Synaptic transmission Postsynaptic currents A1R antagonist Therapeutics. Pharmacology RM1-950 Jinxiu Wang Yanchun Gong Haoyuan Tan Wenxi Li Baiyi Yan Chunfang Cheng Juan Wan Wei Sun Chunhua Yuan Li-Hua Yao Cordycepin suppresses glutamatergic and GABAergic synaptic transmission through activation of A1 adenosine receptor in rat hippocampal CA1 pyramidal neurons
description	Cordycepin (known as 3-deoxyadenosine, CRD), a natural product from the valuable traditional Chinese medicine Cordyceps militaris, has been reported to improve cognitive function and modulate neuroprotective effects on the central nervous system (CNS). However, the modulating mechanisms of cordycepin on information processing in hippocampal CA1 pyramidal neurons are not fully understood. To clarify how cordycepin modulates synaptic responses of pyramidal neurons in rat hippocampal CA1 region, we conducted an electrophysiological experiment using whole-cell patch-clamp technique. The spontaneous and miniature excitatory postsynaptic currents (sEPSCs and mEPSCs, respectively) and the spontaneous and miniature inhibitory postsynaptic currents (sIPSCs and mIPSCs, respectively) recorded by this technique evaluated pure single or multi-synapse responses and enabled us to accurately quantify how cordycepin influenced the pre and postsynaptic aspects of synaptic transmission. The present results showed that cordycepin significantly decreased the frequency of both glutamatergic and GABAergic postsynaptic currents without affecting the amplitude, while these inhibitory effects were antagonized by the A1 adenosine receptor antagonist (DPCPX), but not the A2A (ZM 241385), A2B (MRS1754) and A3 (MRS1191) adenosine receptor antagonists. Taken together, our results suggested that cordycepin had a clear presynaptic effect on glutamatergic and GABAergic transmission, and provided novel evidence that cordycepin suppresses the synaptic transmission through the activation of A1AR.
format	article
author	Jinxiu Wang Yanchun Gong Haoyuan Tan Wenxi Li Baiyi Yan Chunfang Cheng Juan Wan Wei Sun Chunhua Yuan Li-Hua Yao
author_facet	Jinxiu Wang Yanchun Gong Haoyuan Tan Wenxi Li Baiyi Yan Chunfang Cheng Juan Wan Wei Sun Chunhua Yuan Li-Hua Yao
author_sort	Jinxiu Wang
title	Cordycepin suppresses glutamatergic and GABAergic synaptic transmission through activation of A1 adenosine receptor in rat hippocampal CA1 pyramidal neurons
title_short	Cordycepin suppresses glutamatergic and GABAergic synaptic transmission through activation of A1 adenosine receptor in rat hippocampal CA1 pyramidal neurons
title_full	Cordycepin suppresses glutamatergic and GABAergic synaptic transmission through activation of A1 adenosine receptor in rat hippocampal CA1 pyramidal neurons
title_fullStr	Cordycepin suppresses glutamatergic and GABAergic synaptic transmission through activation of A1 adenosine receptor in rat hippocampal CA1 pyramidal neurons
title_full_unstemmed	Cordycepin suppresses glutamatergic and GABAergic synaptic transmission through activation of A1 adenosine receptor in rat hippocampal CA1 pyramidal neurons
title_sort	cordycepin suppresses glutamatergic and gabaergic synaptic transmission through activation of a1 adenosine receptor in rat hippocampal ca1 pyramidal neurons
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/0b5a9b96dc954bf4875e65bfcb2d529c
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Cordycepin suppresses glutamatergic and GABAergic synaptic transmission through activation of A1 adenosine receptor in rat hippocampal CA1 pyramidal neurons

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