SSR and morphological traits based fingerprints and DNA barcodes for varietal identification in rice

Varietal identification has attained prime importance at a global level particularly in the context of plant variety protection. In this study, forty-six traits were characterized for the establishment of the sheer distinctness among eighteen rice varieties. Morphologically, out of forty-six traits,...

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Autores principales:	Rajappa Harisha, Dharavath Bhadru, Satturu Vanisri, Vuppu Gourishanakar, Lakkakula Satish
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	barcodes dus fingerprints molecular fingerprinting rice ssrs varietal identification Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/0b61b6c8abf0485092e3bb26b5ef2f9b
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spelling	oai:doaj.org-article:0b61b6c8abf0485092e3bb26b5ef2f9b2021-11-26T11:19:47ZSSR and morphological traits based fingerprints and DNA barcodes for varietal identification in rice1310-28181314-353010.1080/13102818.2021.1987324https://doaj.org/article/0b61b6c8abf0485092e3bb26b5ef2f9b2021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13102818.2021.1987324https://doaj.org/toc/1310-2818https://doaj.org/toc/1314-3530Varietal identification has attained prime importance at a global level particularly in the context of plant variety protection. In this study, forty-six traits were characterized for the establishment of the sheer distinctness among eighteen rice varieties. Morphologically, out of forty-six traits, twenty-five traits did not display any variation among the varieties studied. Significant variations were observed for 21 traits which are relatively more informative in the identification/characterization. Distinctness, uniformity and stability-based fingerprints specific to the studied varieties were developed based on the variations. A total of 175 simple sequence repeat (SSR) markers were screened for polymorphism; 53 hypervariable polymorphic SSR markers were utilized for fingerprinting of rice varieties. A total of 151 alleles were detected in these 53 SSR markers with an amplification size ranging from 120 to 750 bp and the allelic range of 2–4. The average number of alleles observed was 2.84 per locus. The PIC values of 53 SSRs ranged from 0.03 (RM 12983) to 0.64 (RM 11449) with an average of 0.40. The generated allelic variations of 53 SSR markers for the varieties were translated into DNA barcode profiles by separating the allele size from each SSR locus. Multiplex polymerase chain reaction (PCR) with five markers (RM 21392, RM 1388, RM 6699, RM 25754 and RM 16913) was used to identify rice varieties based on the band positions. This study classifies and identifies rice varieties and is an important reference to test the authenticity and varietal purity of other rice varieties in the future. Supplemental data for this article is available online at https://doi.org/10.1080/13102818.2021.1987324 .Rajappa HarishaDharavath BhadruSatturu VanisriVuppu GourishanakarLakkakula SatishTaylor & Francis Grouparticlebarcodesdus fingerprintsmolecular fingerprintingricessrsvarietal identificationBiotechnologyTP248.13-248.65ENBiotechnology & Biotechnological Equipment, Vol 35, Iss 1, Pp 1461-1473 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	barcodes dus fingerprints molecular fingerprinting rice ssrs varietal identification Biotechnology TP248.13-248.65
spellingShingle	barcodes dus fingerprints molecular fingerprinting rice ssrs varietal identification Biotechnology TP248.13-248.65 Rajappa Harisha Dharavath Bhadru Satturu Vanisri Vuppu Gourishanakar Lakkakula Satish SSR and morphological traits based fingerprints and DNA barcodes for varietal identification in rice
description	Varietal identification has attained prime importance at a global level particularly in the context of plant variety protection. In this study, forty-six traits were characterized for the establishment of the sheer distinctness among eighteen rice varieties. Morphologically, out of forty-six traits, twenty-five traits did not display any variation among the varieties studied. Significant variations were observed for 21 traits which are relatively more informative in the identification/characterization. Distinctness, uniformity and stability-based fingerprints specific to the studied varieties were developed based on the variations. A total of 175 simple sequence repeat (SSR) markers were screened for polymorphism; 53 hypervariable polymorphic SSR markers were utilized for fingerprinting of rice varieties. A total of 151 alleles were detected in these 53 SSR markers with an amplification size ranging from 120 to 750 bp and the allelic range of 2–4. The average number of alleles observed was 2.84 per locus. The PIC values of 53 SSRs ranged from 0.03 (RM 12983) to 0.64 (RM 11449) with an average of 0.40. The generated allelic variations of 53 SSR markers for the varieties were translated into DNA barcode profiles by separating the allele size from each SSR locus. Multiplex polymerase chain reaction (PCR) with five markers (RM 21392, RM 1388, RM 6699, RM 25754 and RM 16913) was used to identify rice varieties based on the band positions. This study classifies and identifies rice varieties and is an important reference to test the authenticity and varietal purity of other rice varieties in the future. Supplemental data for this article is available online at https://doi.org/10.1080/13102818.2021.1987324 .
format	article
author	Rajappa Harisha Dharavath Bhadru Satturu Vanisri Vuppu Gourishanakar Lakkakula Satish
author_facet	Rajappa Harisha Dharavath Bhadru Satturu Vanisri Vuppu Gourishanakar Lakkakula Satish
author_sort	Rajappa Harisha
title	SSR and morphological traits based fingerprints and DNA barcodes for varietal identification in rice
title_short	SSR and morphological traits based fingerprints and DNA barcodes for varietal identification in rice
title_full	SSR and morphological traits based fingerprints and DNA barcodes for varietal identification in rice
title_fullStr	SSR and morphological traits based fingerprints and DNA barcodes for varietal identification in rice
title_full_unstemmed	SSR and morphological traits based fingerprints and DNA barcodes for varietal identification in rice
title_sort	ssr and morphological traits based fingerprints and dna barcodes for varietal identification in rice
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/0b61b6c8abf0485092e3bb26b5ef2f9b
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SSR and morphological traits based fingerprints and DNA barcodes for varietal identification in rice

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