Silencing abnormal wing disc gene of the Asian citrus psyllid, Diaphorina citri disrupts adult wing development and increases nymph mortality.

Huanglongbing (HLB) causes considerable economic losses to citrus industries worldwide. Its management depends on controlling of the Asian citrus Psyllid (ACP), the vector of the bacterium, Candidatus Liberibacter asiaticus (CLas), the causal agent of HLB. Silencing genes by RNA interference (RNAi)...

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Autores principales:	Ibrahim El-Shesheny, Subhas Hajeri, Ibrahim El-Hawary, Siddarame Gowda, Nabil Killiny
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0b654b0b59df4c0d8631374345df156b
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spelling	oai:doaj.org-article:0b654b0b59df4c0d8631374345df156b2021-11-18T07:43:52ZSilencing abnormal wing disc gene of the Asian citrus psyllid, Diaphorina citri disrupts adult wing development and increases nymph mortality.1932-620310.1371/journal.pone.0065392https://doaj.org/article/0b654b0b59df4c0d8631374345df156b2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23734251/?tool=EBIhttps://doaj.org/toc/1932-6203Huanglongbing (HLB) causes considerable economic losses to citrus industries worldwide. Its management depends on controlling of the Asian citrus Psyllid (ACP), the vector of the bacterium, Candidatus Liberibacter asiaticus (CLas), the causal agent of HLB. Silencing genes by RNA interference (RNAi) is a promising tool to explore gene functions as well as control pests. In the current study, abnormal wing disc (awd) gene associated with wing development in insects is used to interfere with the flight of psyllids. Our study showed that transcription of awd is development-dependent and the highest level was found in the last instar (5(th)) of the nymphal stage. Micro-application (topical application) of dsRNA to 5(th) instar of nymphs caused significant nymphal mortality and adult wing-malformation. These adverse effects in ACP were positively correlated with the amounts of dsRNA used. A qRT-PCR analysis confirmed the dsRNA-mediated transcriptional down-regulation of the awd gene. Significant down-regulation was required to induce a wing-malformed phenotype. No effect was found when dsRNA-gfp was used, indicating the specific effect of dsRNA-awd. Our findings suggest a role for awd in ACP wing development and metamorphosis. awd could serve as a potential target for insect management either via direct application of dsRNA or by producing transgenic plants expressing dsRNA-awd. These strategies will help to mitigate HLB by controlling ACP.Ibrahim El-SheshenySubhas HajeriIbrahim El-HawarySiddarame GowdaNabil KillinyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e65392 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ibrahim El-Shesheny Subhas Hajeri Ibrahim El-Hawary Siddarame Gowda Nabil Killiny Silencing abnormal wing disc gene of the Asian citrus psyllid, Diaphorina citri disrupts adult wing development and increases nymph mortality.
description	Huanglongbing (HLB) causes considerable economic losses to citrus industries worldwide. Its management depends on controlling of the Asian citrus Psyllid (ACP), the vector of the bacterium, Candidatus Liberibacter asiaticus (CLas), the causal agent of HLB. Silencing genes by RNA interference (RNAi) is a promising tool to explore gene functions as well as control pests. In the current study, abnormal wing disc (awd) gene associated with wing development in insects is used to interfere with the flight of psyllids. Our study showed that transcription of awd is development-dependent and the highest level was found in the last instar (5(th)) of the nymphal stage. Micro-application (topical application) of dsRNA to 5(th) instar of nymphs caused significant nymphal mortality and adult wing-malformation. These adverse effects in ACP were positively correlated with the amounts of dsRNA used. A qRT-PCR analysis confirmed the dsRNA-mediated transcriptional down-regulation of the awd gene. Significant down-regulation was required to induce a wing-malformed phenotype. No effect was found when dsRNA-gfp was used, indicating the specific effect of dsRNA-awd. Our findings suggest a role for awd in ACP wing development and metamorphosis. awd could serve as a potential target for insect management either via direct application of dsRNA or by producing transgenic plants expressing dsRNA-awd. These strategies will help to mitigate HLB by controlling ACP.
format	article
author	Ibrahim El-Shesheny Subhas Hajeri Ibrahim El-Hawary Siddarame Gowda Nabil Killiny
author_facet	Ibrahim El-Shesheny Subhas Hajeri Ibrahim El-Hawary Siddarame Gowda Nabil Killiny
author_sort	Ibrahim El-Shesheny
title	Silencing abnormal wing disc gene of the Asian citrus psyllid, Diaphorina citri disrupts adult wing development and increases nymph mortality.
title_short	Silencing abnormal wing disc gene of the Asian citrus psyllid, Diaphorina citri disrupts adult wing development and increases nymph mortality.
title_full	Silencing abnormal wing disc gene of the Asian citrus psyllid, Diaphorina citri disrupts adult wing development and increases nymph mortality.
title_fullStr	Silencing abnormal wing disc gene of the Asian citrus psyllid, Diaphorina citri disrupts adult wing development and increases nymph mortality.
title_full_unstemmed	Silencing abnormal wing disc gene of the Asian citrus psyllid, Diaphorina citri disrupts adult wing development and increases nymph mortality.
title_sort	silencing abnormal wing disc gene of the asian citrus psyllid, diaphorina citri disrupts adult wing development and increases nymph mortality.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/0b654b0b59df4c0d8631374345df156b
work_keys_str_mv	AT ibrahimelshesheny silencingabnormalwingdiscgeneoftheasiancitruspsylliddiaphorinacitridisruptsadultwingdevelopmentandincreasesnymphmortality AT subhashajeri silencingabnormalwingdiscgeneoftheasiancitruspsylliddiaphorinacitridisruptsadultwingdevelopmentandincreasesnymphmortality AT ibrahimelhawary silencingabnormalwingdiscgeneoftheasiancitruspsylliddiaphorinacitridisruptsadultwingdevelopmentandincreasesnymphmortality AT siddaramegowda silencingabnormalwingdiscgeneoftheasiancitruspsylliddiaphorinacitridisruptsadultwingdevelopmentandincreasesnymphmortality AT nabilkilliny silencingabnormalwingdiscgeneoftheasiancitruspsylliddiaphorinacitridisruptsadultwingdevelopmentandincreasesnymphmortality
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Silencing abnormal wing disc gene of the Asian citrus psyllid, Diaphorina citri disrupts adult wing development and increases nymph mortality.

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