In situ exploration of characteristics of macropinocytosis and size range of internalized substances in cells by 3D-structured illumination microscopy

Jing Jin,1 Yanting Shen,2 Biao Zhang,1 Rong Deng,3 Dianshuai Huang,1 Tianqi Lu,1 Fei Sun,1,* Shuping Xu,2,* Chongyang Liang1,*1Institute of Frontier Medical Science, Jilin University, Changchun 130021, Jilin, People’s Republic of China; 2State Key Lab of Supramolecular Structure and Materi...

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Autores principales:	Jin J, Shen Y, Zhang B, Deng R, Huang D, Lu T, Sun F, Xu S, Liang C
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	nano materials macropinocytosis 3D-SIM internalization Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0b666cadeae84e66818b0440e6dacdeb
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spelling	oai:doaj.org-article:0b666cadeae84e66818b0440e6dacdeb2021-12-02T08:02:47ZIn situ exploration of characteristics of macropinocytosis and size range of internalized substances in cells by 3D-structured illumination microscopy1178-2013https://doaj.org/article/0b666cadeae84e66818b0440e6dacdeb2018-09-01T00:00:00Zhttps://www.dovepress.com/in-situ-exploration-of-characteristics-of-macropinocytosis-and-size-ra-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Jing Jin,1 Yanting Shen,2 Biao Zhang,1 Rong Deng,3 Dianshuai Huang,1 Tianqi Lu,1 Fei Sun,1,* Shuping Xu,2,* Chongyang Liang1,1Institute of Frontier Medical Science, Jilin University, Changchun 130021, Jilin, People’s Republic of China; 2State Key Lab of Supramolecular Structure and Materials, Jilin University, Changchun 130021, Jilin, People’s Republic of China; 3International Joint Research Laboratory of Nano-Micro Architecture Chemistry (NMAC), Jilin University, Changchun 130021, Jilin, People’s Republic of ChinaThese authors contributed equally to this workBackground: Macropinocytosis can occur in various types of cells and displays multiple functions. However, real-time observation and characterization of the structures of macropinocytosis on the surface of the cell membrane is not yet possible.Materials and methods: Here, we establish a real-time live cell surface imaging method using three-dimensional-structured illumination microscopy. Based on this, observation of the dynamic macropinocytosis process and morphological data of internalized structures on the surface of pancreatic cancer cells were achieved during macropinocytosis. Next, different-sized silica nanoparticles (SiO2 NPs) were used as the scale for identifying the size range of internalized substances of macropinocytosis in pancreatic cancer cells.Results and conclusion: Our study not only provides a practical method and more structural data for further investigation of macropinocytosis, but also makes deeper understanding of the cell response toward nanomaterials as well as nanodrugs possible.Keywords: nanomaterials, macropinocytosis, 3D-SIM, internalizationJin JShen YZhang BDeng RHuang DLu TSun FXu SLiang CDove Medical Pressarticlenano materialsmacropinocytosis3D-SIMinternalizationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 5321-5333 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	nano materials macropinocytosis 3D-SIM internalization Medicine (General) R5-920
spellingShingle	nano materials macropinocytosis 3D-SIM internalization Medicine (General) R5-920 Jin J Shen Y Zhang B Deng R Huang D Lu T Sun F Xu S Liang C In situ exploration of characteristics of macropinocytosis and size range of internalized substances in cells by 3D-structured illumination microscopy
description	Jing Jin,1 Yanting Shen,2 Biao Zhang,1 Rong Deng,3 Dianshuai Huang,1 Tianqi Lu,1 Fei Sun,1,* Shuping Xu,2,* Chongyang Liang1,1Institute of Frontier Medical Science, Jilin University, Changchun 130021, Jilin, People’s Republic of China; 2State Key Lab of Supramolecular Structure and Materials, Jilin University, Changchun 130021, Jilin, People’s Republic of China; 3International Joint Research Laboratory of Nano-Micro Architecture Chemistry (NMAC), Jilin University, Changchun 130021, Jilin, People’s Republic of ChinaThese authors contributed equally to this workBackground: Macropinocytosis can occur in various types of cells and displays multiple functions. However, real-time observation and characterization of the structures of macropinocytosis on the surface of the cell membrane is not yet possible.Materials and methods: Here, we establish a real-time live cell surface imaging method using three-dimensional-structured illumination microscopy. Based on this, observation of the dynamic macropinocytosis process and morphological data of internalized structures on the surface of pancreatic cancer cells were achieved during macropinocytosis. Next, different-sized silica nanoparticles (SiO2 NPs) were used as the scale for identifying the size range of internalized substances of macropinocytosis in pancreatic cancer cells.Results and conclusion: Our study not only provides a practical method and more structural data for further investigation of macropinocytosis, but also makes deeper understanding of the cell response toward nanomaterials as well as nanodrugs possible.Keywords: nanomaterials, macropinocytosis, 3D-SIM, internalization
format	article
author	Jin J Shen Y Zhang B Deng R Huang D Lu T Sun F Xu S Liang C
author_facet	Jin J Shen Y Zhang B Deng R Huang D Lu T Sun F Xu S Liang C
author_sort	Jin J
title	In situ exploration of characteristics of macropinocytosis and size range of internalized substances in cells by 3D-structured illumination microscopy
title_short	In situ exploration of characteristics of macropinocytosis and size range of internalized substances in cells by 3D-structured illumination microscopy
title_full	In situ exploration of characteristics of macropinocytosis and size range of internalized substances in cells by 3D-structured illumination microscopy
title_fullStr	In situ exploration of characteristics of macropinocytosis and size range of internalized substances in cells by 3D-structured illumination microscopy
title_full_unstemmed	In situ exploration of characteristics of macropinocytosis and size range of internalized substances in cells by 3D-structured illumination microscopy
title_sort	in situ exploration of characteristics of macropinocytosis and size range of internalized substances in cells by 3d-structured illumination microscopy
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/0b666cadeae84e66818b0440e6dacdeb
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In situ exploration of characteristics of macropinocytosis and size range of internalized substances in cells by 3D-structured illumination microscopy

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