Transcriptomic analyses reveal comprehensive responses of insect hemocytes to mycopathogen Beauveria bassiana, and fungal virulence-related cell wall protein assists pathogen to evade host cellular defense

Entomopathogenic fungi naturally infect insect hosts in environment. Fungal invasion and host immune defense are still in the progress of co-evolution. In this study, entomopathogenic fungus Beauveria bassiana and lepidopteran insect Galleria mellonella were used to investigate host cellular immunit...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jin-Li Ding, Jia Hou, Ming-Guang Feng, Sheng-Hua Ying
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	insect immunity hemocyte response encapsulation cell-wall protein entomopathogenic fungus beauveria bassiana virulence Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/0b6b44bf4bca42ca950665a29d2b3786
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0b6b44bf4bca42ca950665a29d2b3786
record_format	dspace
spelling	oai:doaj.org-article:0b6b44bf4bca42ca950665a29d2b37862021-11-17T14:21:59ZTranscriptomic analyses reveal comprehensive responses of insect hemocytes to mycopathogen Beauveria bassiana, and fungal virulence-related cell wall protein assists pathogen to evade host cellular defense2150-55942150-560810.1080/21505594.2020.1827886https://doaj.org/article/0b6b44bf4bca42ca950665a29d2b37862020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1827886https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608Entomopathogenic fungi naturally infect insect hosts in environment. Fungal invasion and host immune defense are still in the progress of co-evolution. In this study, entomopathogenic fungus Beauveria bassiana and lepidopteran insect Galleria mellonella were used to investigate host cellular immunity and fungal strategy to evade host defense. First of all, genome-wide expression revealed the transcriptomic responses of hemocytes to insect mycopathogen, which dynamically varied during infection process. Enrichment analysis indicated that differentially expressed genes were primarily involved in metabolism, cellular process and immune system. Notably, cellular response involved a series of hydrolytic enzyme and antimicrobial peptide genes which were sorted together in clustering analysis. In B. bassiana, a cell-wall protein gene (BbCwp) contributes to fungal development in host hemocoel and virulence. RT-qPCR analyses indicated that infection by ΔBbCwp mutant strain caused the up-regulated expression of a series of immunity-related genes, including β-1, 3-glucan recognition protein, hydrolytic enzyme and antimicrobial peptide genes. Disruption of BbCwp resulted in a significant change in conidial lectin-binding feature and the enhanced encapsulation by the host hemocytes. After being treated with hydrolytic enzymes, ΔBbCwp mutant displayed a significantly enhanced sensitivity to osmotic and oxidative stresses. In conclusion, fungal invasion initiates comprehensive physiological responses in the host hemocytes. For mycopathogen, cell-wall protein plays an important role in fungal evasion of immunity defense and colonization in host. Our studies provide an initial framework for exploring more mechanistic details about the fungus–host interaction.Jin-Li DingJia HouMing-Guang FengSheng-Hua YingTaylor & Francis Grouparticleinsect immunityhemocyte responseencapsulationcell-wall proteinentomopathogenic fungusbeauveria bassianavirulenceInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 1352-1365 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	insect immunity hemocyte response encapsulation cell-wall protein entomopathogenic fungus beauveria bassiana virulence Infectious and parasitic diseases RC109-216
spellingShingle	insect immunity hemocyte response encapsulation cell-wall protein entomopathogenic fungus beauveria bassiana virulence Infectious and parasitic diseases RC109-216 Jin-Li Ding Jia Hou Ming-Guang Feng Sheng-Hua Ying Transcriptomic analyses reveal comprehensive responses of insect hemocytes to mycopathogen Beauveria bassiana, and fungal virulence-related cell wall protein assists pathogen to evade host cellular defense
description	Entomopathogenic fungi naturally infect insect hosts in environment. Fungal invasion and host immune defense are still in the progress of co-evolution. In this study, entomopathogenic fungus Beauveria bassiana and lepidopteran insect Galleria mellonella were used to investigate host cellular immunity and fungal strategy to evade host defense. First of all, genome-wide expression revealed the transcriptomic responses of hemocytes to insect mycopathogen, which dynamically varied during infection process. Enrichment analysis indicated that differentially expressed genes were primarily involved in metabolism, cellular process and immune system. Notably, cellular response involved a series of hydrolytic enzyme and antimicrobial peptide genes which were sorted together in clustering analysis. In B. bassiana, a cell-wall protein gene (BbCwp) contributes to fungal development in host hemocoel and virulence. RT-qPCR analyses indicated that infection by ΔBbCwp mutant strain caused the up-regulated expression of a series of immunity-related genes, including β-1, 3-glucan recognition protein, hydrolytic enzyme and antimicrobial peptide genes. Disruption of BbCwp resulted in a significant change in conidial lectin-binding feature and the enhanced encapsulation by the host hemocytes. After being treated with hydrolytic enzymes, ΔBbCwp mutant displayed a significantly enhanced sensitivity to osmotic and oxidative stresses. In conclusion, fungal invasion initiates comprehensive physiological responses in the host hemocytes. For mycopathogen, cell-wall protein plays an important role in fungal evasion of immunity defense and colonization in host. Our studies provide an initial framework for exploring more mechanistic details about the fungus–host interaction.
format	article
author	Jin-Li Ding Jia Hou Ming-Guang Feng Sheng-Hua Ying
author_facet	Jin-Li Ding Jia Hou Ming-Guang Feng Sheng-Hua Ying
author_sort	Jin-Li Ding
title	Transcriptomic analyses reveal comprehensive responses of insect hemocytes to mycopathogen Beauveria bassiana, and fungal virulence-related cell wall protein assists pathogen to evade host cellular defense
title_short	Transcriptomic analyses reveal comprehensive responses of insect hemocytes to mycopathogen Beauveria bassiana, and fungal virulence-related cell wall protein assists pathogen to evade host cellular defense
title_full	Transcriptomic analyses reveal comprehensive responses of insect hemocytes to mycopathogen Beauveria bassiana, and fungal virulence-related cell wall protein assists pathogen to evade host cellular defense
title_fullStr	Transcriptomic analyses reveal comprehensive responses of insect hemocytes to mycopathogen Beauveria bassiana, and fungal virulence-related cell wall protein assists pathogen to evade host cellular defense
title_full_unstemmed	Transcriptomic analyses reveal comprehensive responses of insect hemocytes to mycopathogen Beauveria bassiana, and fungal virulence-related cell wall protein assists pathogen to evade host cellular defense
title_sort	transcriptomic analyses reveal comprehensive responses of insect hemocytes to mycopathogen beauveria bassiana, and fungal virulence-related cell wall protein assists pathogen to evade host cellular defense
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/0b6b44bf4bca42ca950665a29d2b3786
work_keys_str_mv	AT jinliding transcriptomicanalysesrevealcomprehensiveresponsesofinsecthemocytestomycopathogenbeauveriabassianaandfungalvirulencerelatedcellwallproteinassistspathogentoevadehostcellulardefense AT jiahou transcriptomicanalysesrevealcomprehensiveresponsesofinsecthemocytestomycopathogenbeauveriabassianaandfungalvirulencerelatedcellwallproteinassistspathogentoevadehostcellulardefense AT mingguangfeng transcriptomicanalysesrevealcomprehensiveresponsesofinsecthemocytestomycopathogenbeauveriabassianaandfungalvirulencerelatedcellwallproteinassistspathogentoevadehostcellulardefense AT shenghuaying transcriptomicanalysesrevealcomprehensiveresponsesofinsecthemocytestomycopathogenbeauveriabassianaandfungalvirulencerelatedcellwallproteinassistspathogentoevadehostcellulardefense
_version_	1718425396701036544

Transcriptomic analyses reveal comprehensive responses of insect hemocytes to mycopathogen Beauveria bassiana, and fungal virulence-related cell wall protein assists pathogen to evade host cellular defense

Ejemplares similares