Effect of type 2 diabetic serum on the behavior of Wharton's jelly-derived mesenchymal stem cells in vitro

Objective: Wharton jelly-derived mesenchymal stem cells (WJMSCs) exhibit multilineage differentiation potential and can be used to treat multiple organs. However, diabetes affects the repair capability of MSCs. The aim of this study was to evaluate the effect of diabetic patient-derived serum on WJM...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Fatima Ali, Fehmina Aziz, Nadia Wajid
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2017
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0b781e2dfba040d69cb0c164845da6d0
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0b781e2dfba040d69cb0c164845da6d0
record_format	dspace
spelling	oai:doaj.org-article:0b781e2dfba040d69cb0c164845da6d02021-12-02T13:36:22ZEffect of type 2 diabetic serum on the behavior of Wharton's jelly-derived mesenchymal stem cells in vitro2095-882X10.1016/j.cdtm.2017.02.006https://doaj.org/article/0b781e2dfba040d69cb0c164845da6d02017-06-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2095882X16301037https://doaj.org/toc/2095-882XObjective: Wharton jelly-derived mesenchymal stem cells (WJMSCs) exhibit multilineage differentiation potential and can be used to treat multiple organs. However, diabetes affects the repair capability of MSCs. The aim of this study was to evaluate the effect of diabetic patient-derived serum on WJMSC behavior. Methods: WJMSCs at passage 3 were treated with serum derived from type 2 diabetic patients. WJMSCs were characterized for surface markers expression by using immunocytochemistry technique. The effects on cell viability, proliferation, cell death rate, and vascular endothelial growth factor level were assessed by crystal violet staining, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, lactate dehydrogenase assay, and enzyme-linked immuno-sorbent assay, respectively. Oxidative stress was assessed by the estimation of free radical species malondialdehyde (MDA) and enzymes glutathione (GSH), catalase, and superoxide dismutase (SOD). Results: WJMSCs isolated in this study were positive for CD29, CD49, CD73, CD90, CD105, and SSEA4 and negative for CD45 and CD34. Under diabetic stress conditions, WJMSCs showed low viability and high lactate dehydrogenase release. A low level of vascular endothelial growth factor was also observed after diabetic serum treatment. Antioxidant level was also lower in diabetic serum-treated WJMSCs compared to in normal serum-treated WJMSCs. Conclusion: The results of the present study suggest that pre-treatment of WJMSCs with type 2 diabetic serum decreases the survival of WJMSCs. The findings of this study provide insight into diabetes-induced harmful effects on WJMSCs. Keywords: Wharton jelly-derived mesenchymal stem cells, Type 2 diabetes mellitus, Oxidative stress, Vascular endothelial growth factorFatima AliFehmina AzizNadia WajidKeAi Communications Co., Ltd.articleMedicine (General)R5-920ENChronic Diseases and Translational Medicine, Vol 3, Iss 2, Pp 105-111 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Fatima Ali Fehmina Aziz Nadia Wajid Effect of type 2 diabetic serum on the behavior of Wharton's jelly-derived mesenchymal stem cells in vitro
description	Objective: Wharton jelly-derived mesenchymal stem cells (WJMSCs) exhibit multilineage differentiation potential and can be used to treat multiple organs. However, diabetes affects the repair capability of MSCs. The aim of this study was to evaluate the effect of diabetic patient-derived serum on WJMSC behavior. Methods: WJMSCs at passage 3 were treated with serum derived from type 2 diabetic patients. WJMSCs were characterized for surface markers expression by using immunocytochemistry technique. The effects on cell viability, proliferation, cell death rate, and vascular endothelial growth factor level were assessed by crystal violet staining, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, lactate dehydrogenase assay, and enzyme-linked immuno-sorbent assay, respectively. Oxidative stress was assessed by the estimation of free radical species malondialdehyde (MDA) and enzymes glutathione (GSH), catalase, and superoxide dismutase (SOD). Results: WJMSCs isolated in this study were positive for CD29, CD49, CD73, CD90, CD105, and SSEA4 and negative for CD45 and CD34. Under diabetic stress conditions, WJMSCs showed low viability and high lactate dehydrogenase release. A low level of vascular endothelial growth factor was also observed after diabetic serum treatment. Antioxidant level was also lower in diabetic serum-treated WJMSCs compared to in normal serum-treated WJMSCs. Conclusion: The results of the present study suggest that pre-treatment of WJMSCs with type 2 diabetic serum decreases the survival of WJMSCs. The findings of this study provide insight into diabetes-induced harmful effects on WJMSCs. Keywords: Wharton jelly-derived mesenchymal stem cells, Type 2 diabetes mellitus, Oxidative stress, Vascular endothelial growth factor
format	article
author	Fatima Ali Fehmina Aziz Nadia Wajid
author_facet	Fatima Ali Fehmina Aziz Nadia Wajid
author_sort	Fatima Ali
title	Effect of type 2 diabetic serum on the behavior of Wharton's jelly-derived mesenchymal stem cells in vitro
title_short	Effect of type 2 diabetic serum on the behavior of Wharton's jelly-derived mesenchymal stem cells in vitro
title_full	Effect of type 2 diabetic serum on the behavior of Wharton's jelly-derived mesenchymal stem cells in vitro
title_fullStr	Effect of type 2 diabetic serum on the behavior of Wharton's jelly-derived mesenchymal stem cells in vitro
title_full_unstemmed	Effect of type 2 diabetic serum on the behavior of Wharton's jelly-derived mesenchymal stem cells in vitro
title_sort	effect of type 2 diabetic serum on the behavior of wharton's jelly-derived mesenchymal stem cells in vitro
publisher	KeAi Communications Co., Ltd.
publishDate	2017
url	https://doaj.org/article/0b781e2dfba040d69cb0c164845da6d0
work_keys_str_mv	AT fatimaali effectoftype2diabeticserumonthebehaviorofwhartonsjellyderivedmesenchymalstemcellsinvitro AT fehminaaziz effectoftype2diabeticserumonthebehaviorofwhartonsjellyderivedmesenchymalstemcellsinvitro AT nadiawajid effectoftype2diabeticserumonthebehaviorofwhartonsjellyderivedmesenchymalstemcellsinvitro
_version_	1718392657332404224

Effect of type 2 diabetic serum on the behavior of Wharton's jelly-derived mesenchymal stem cells in vitro

Ejemplares similares