Occurrence of chitinolytic bacteria in shrimp rusip and measurement of their chitin-degrading enzyme activities

Puspita ID, Wardani A, Puspitasari ROA, Nugraheni PS, Putra MMP, Pudjiraharti S, Ustadi. 2017. Occurrence of chitinolytic bacteria in shrimp rusip and measurement of their chitin-degrading enzyme activities. Biodiversitas 18: 1275-1281. The objectives of this research were to isolate chitinolytic ba...

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Autores principales:	INDUN DEWI PUSPITA, ARI WARDANI, RR. OKI ARUM PUSPITASARI, PRIHATI SIH NUGRAHENI, MGS. MUHAMMAD PRIMA PUTRA, SRI PUDJIRAHARTI, USTADI USTADI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2017
Materias:	chitinase activity chitinolytic bacteria shrimp rusip Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/0b7e6ad5ff23475fbb0b5bd1e8c6e834
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spelling	oai:doaj.org-article:0b7e6ad5ff23475fbb0b5bd1e8c6e8342021-11-16T13:56:34ZOccurrence of chitinolytic bacteria in shrimp rusip and measurement of their chitin-degrading enzyme activities1412-033X2085-472210.13057/biodiv/d180354https://doaj.org/article/0b7e6ad5ff23475fbb0b5bd1e8c6e8342017-07-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2054https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Puspita ID, Wardani A, Puspitasari ROA, Nugraheni PS, Putra MMP, Pudjiraharti S, Ustadi. 2017. Occurrence of chitinolytic bacteria in shrimp rusip and measurement of their chitin-degrading enzyme activities. Biodiversitas 18: 1275-1281. The objectives of this research were to isolate chitinolytic bacteria from shrimp rusip (an Indonesian traditional fermented shrimp product), identify bacterial isolates showing high chitinolytic activity, and determine the chitinolytic activity of these isolates. There were 44 chitinolytic bacteria isolated from shrimp rusip: 39 isolates of Gram-positive bacteria and 5 isolates of Gram-negative bacteria. The quantitative method we used to evaluate chitin-degrading enzyme activity measured the amount of N-acetylglucosamine produced from the reaction of crude enzyme and colloidal chitin. Seven isolates showing highest chitinolytic activity were Bacillus cereus (KKT 1, KKT 14, and KKT 19), Bacillus thuringiensis (KKT 6), Enterobacter cloacae (LCK 20), Pseudomonas stutzeri (LCK 17), and Stenotrophomonas maltophilia (THK1). The highest chitinolytic activity showed by KKT 1, KKT 6, KKT 14, KKT 19, and THK 1 were 0.0016 U/mL (at 72 hours); 0.0018 U/mL (at 48 hours); 0.0019 U/mL (at 48 hours); 0.0033 U/mL (at 48 hours); and 0.0032 U/mL (at 24 hours), respectively.INDUN DEWI PUSPITAARI WARDANIRR. OKI ARUM PUSPITASARIPRIHATI SIH NUGRAHENIMGS. MUHAMMAD PRIMA PUTRASRI PUDJIRAHARTIUSTADI USTADIMBI & UNS Soloarticlechitinase activitychitinolytic bacteriashrimp rusipBiology (General)QH301-705.5ENBiodiversitas, Vol 18, Iss 3, Pp 1275-1281 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	chitinase activity chitinolytic bacteria shrimp rusip Biology (General) QH301-705.5
spellingShingle	chitinase activity chitinolytic bacteria shrimp rusip Biology (General) QH301-705.5 INDUN DEWI PUSPITA ARI WARDANI RR. OKI ARUM PUSPITASARI PRIHATI SIH NUGRAHENI MGS. MUHAMMAD PRIMA PUTRA SRI PUDJIRAHARTI USTADI USTADI Occurrence of chitinolytic bacteria in shrimp rusip and measurement of their chitin-degrading enzyme activities
description	Puspita ID, Wardani A, Puspitasari ROA, Nugraheni PS, Putra MMP, Pudjiraharti S, Ustadi. 2017. Occurrence of chitinolytic bacteria in shrimp rusip and measurement of their chitin-degrading enzyme activities. Biodiversitas 18: 1275-1281. The objectives of this research were to isolate chitinolytic bacteria from shrimp rusip (an Indonesian traditional fermented shrimp product), identify bacterial isolates showing high chitinolytic activity, and determine the chitinolytic activity of these isolates. There were 44 chitinolytic bacteria isolated from shrimp rusip: 39 isolates of Gram-positive bacteria and 5 isolates of Gram-negative bacteria. The quantitative method we used to evaluate chitin-degrading enzyme activity measured the amount of N-acetylglucosamine produced from the reaction of crude enzyme and colloidal chitin. Seven isolates showing highest chitinolytic activity were Bacillus cereus (KKT 1, KKT 14, and KKT 19), Bacillus thuringiensis (KKT 6), Enterobacter cloacae (LCK 20), Pseudomonas stutzeri (LCK 17), and Stenotrophomonas maltophilia (THK1). The highest chitinolytic activity showed by KKT 1, KKT 6, KKT 14, KKT 19, and THK 1 were 0.0016 U/mL (at 72 hours); 0.0018 U/mL (at 48 hours); 0.0019 U/mL (at 48 hours); 0.0033 U/mL (at 48 hours); and 0.0032 U/mL (at 24 hours), respectively.
format	article
author	INDUN DEWI PUSPITA ARI WARDANI RR. OKI ARUM PUSPITASARI PRIHATI SIH NUGRAHENI MGS. MUHAMMAD PRIMA PUTRA SRI PUDJIRAHARTI USTADI USTADI
author_facet	INDUN DEWI PUSPITA ARI WARDANI RR. OKI ARUM PUSPITASARI PRIHATI SIH NUGRAHENI MGS. MUHAMMAD PRIMA PUTRA SRI PUDJIRAHARTI USTADI USTADI
author_sort	INDUN DEWI PUSPITA
title	Occurrence of chitinolytic bacteria in shrimp rusip and measurement of their chitin-degrading enzyme activities
title_short	Occurrence of chitinolytic bacteria in shrimp rusip and measurement of their chitin-degrading enzyme activities
title_full	Occurrence of chitinolytic bacteria in shrimp rusip and measurement of their chitin-degrading enzyme activities
title_fullStr	Occurrence of chitinolytic bacteria in shrimp rusip and measurement of their chitin-degrading enzyme activities
title_full_unstemmed	Occurrence of chitinolytic bacteria in shrimp rusip and measurement of their chitin-degrading enzyme activities
title_sort	occurrence of chitinolytic bacteria in shrimp rusip and measurement of their chitin-degrading enzyme activities
publisher	MBI & UNS Solo
publishDate	2017
url	https://doaj.org/article/0b7e6ad5ff23475fbb0b5bd1e8c6e834
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Occurrence of chitinolytic bacteria in shrimp rusip and measurement of their chitin-degrading enzyme activities

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