A rapid, low-cost, and microfluidic chip-based system for parallel identification of multiple pathogens related to clinical pneumonia
Abstract An air-insulated microfluidic chip was designed for the automatic centrifugal distribution of samples to 24-test cells, enabling the parallel identification of multiple clinical pneumonia-related pathogens in 1.45-μL reactions without cross-contamination in 45 min. A portable nucleic acid a...
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Nature Portfolio
2017
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oai:doaj.org-article:0b833c04b3b0432893c3ccc344f087b32021-12-02T15:05:31ZA rapid, low-cost, and microfluidic chip-based system for parallel identification of multiple pathogens related to clinical pneumonia10.1038/s41598-017-06739-22045-2322https://doaj.org/article/0b833c04b3b0432893c3ccc344f087b32017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06739-2https://doaj.org/toc/2045-2322Abstract An air-insulated microfluidic chip was designed for the automatic centrifugal distribution of samples to 24-test cells, enabling the parallel identification of multiple clinical pneumonia-related pathogens in 1.45-μL reactions without cross-contamination in 45 min. A portable nucleic acid analyzer that integrates mechanical, confocal optical, electronic, and software functions was also developed to collect fluorescence data in a Ø3 mm imaging field near the optical diffraction limit for highly sensitive fluorescence detection of nucleic acid amplification in real time. This microfluidic chip-based portable nucleic acid analyzer could detect low abundance nucleic acids present at as few as 10 copies. In a blinded experiment, specific identification of Mycoplasma pneumoniae, Staphylococcus aureus, and methicillin-resistant S. aureus was achieved with 229 clinical patient sputum samples. The total coincidence rate of our system and traditional RT-PCR with an ABI 7500 was 99.56%. Four samples accounting for the 0.44% inconformity were retested by gene sequencing, revealing that our system reported the correct results. This novel microfluidic chip-based detection system is cost-effective, rapid, sensitive, specific, and has a relatively high throughput for parallel identification, which is especially suitable for resource-limited facilities/areas and point-of-care testing.Guoliang HuangQin HuangLan XieGuangxin XiangLei WangHui XuLi MaXianbo LuoJuan XinXinying ZhouXiangyu JinLei ZhangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017) |
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DOAJ |
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Medicine R Science Q |
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Medicine R Science Q Guoliang Huang Qin Huang Lan Xie Guangxin Xiang Lei Wang Hui Xu Li Ma Xianbo Luo Juan Xin Xinying Zhou Xiangyu Jin Lei Zhang A rapid, low-cost, and microfluidic chip-based system for parallel identification of multiple pathogens related to clinical pneumonia |
| description |
Abstract An air-insulated microfluidic chip was designed for the automatic centrifugal distribution of samples to 24-test cells, enabling the parallel identification of multiple clinical pneumonia-related pathogens in 1.45-μL reactions without cross-contamination in 45 min. A portable nucleic acid analyzer that integrates mechanical, confocal optical, electronic, and software functions was also developed to collect fluorescence data in a Ø3 mm imaging field near the optical diffraction limit for highly sensitive fluorescence detection of nucleic acid amplification in real time. This microfluidic chip-based portable nucleic acid analyzer could detect low abundance nucleic acids present at as few as 10 copies. In a blinded experiment, specific identification of Mycoplasma pneumoniae, Staphylococcus aureus, and methicillin-resistant S. aureus was achieved with 229 clinical patient sputum samples. The total coincidence rate of our system and traditional RT-PCR with an ABI 7500 was 99.56%. Four samples accounting for the 0.44% inconformity were retested by gene sequencing, revealing that our system reported the correct results. This novel microfluidic chip-based detection system is cost-effective, rapid, sensitive, specific, and has a relatively high throughput for parallel identification, which is especially suitable for resource-limited facilities/areas and point-of-care testing. |
| format |
article |
| author |
Guoliang Huang Qin Huang Lan Xie Guangxin Xiang Lei Wang Hui Xu Li Ma Xianbo Luo Juan Xin Xinying Zhou Xiangyu Jin Lei Zhang |
| author_facet |
Guoliang Huang Qin Huang Lan Xie Guangxin Xiang Lei Wang Hui Xu Li Ma Xianbo Luo Juan Xin Xinying Zhou Xiangyu Jin Lei Zhang |
| author_sort |
Guoliang Huang |
| title |
A rapid, low-cost, and microfluidic chip-based system for parallel identification of multiple pathogens related to clinical pneumonia |
| title_short |
A rapid, low-cost, and microfluidic chip-based system for parallel identification of multiple pathogens related to clinical pneumonia |
| title_full |
A rapid, low-cost, and microfluidic chip-based system for parallel identification of multiple pathogens related to clinical pneumonia |
| title_fullStr |
A rapid, low-cost, and microfluidic chip-based system for parallel identification of multiple pathogens related to clinical pneumonia |
| title_full_unstemmed |
A rapid, low-cost, and microfluidic chip-based system for parallel identification of multiple pathogens related to clinical pneumonia |
| title_sort |
rapid, low-cost, and microfluidic chip-based system for parallel identification of multiple pathogens related to clinical pneumonia |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/0b833c04b3b0432893c3ccc344f087b3 |
| work_keys_str_mv |
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1718388747790188544 |