MicroRNA expression profiles in chronic epilepsy rats and neuroprotection from seizures by targeting miR-344a

Xixia Liu,1,2 Yuhan Liao,1 Xiuxiu Wang,1 Donghua Zou,1 Chun Luo,1 Chongdong Jian,1 Yuan Wu1 1Department of Neurology, First Affiliated Hospital of Guangxi Medical University, 2Department of Rehabilitation, People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning, China Abstract: Mi...

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Autores principales:	Liu XX, Liao YH, Wang XX, Zou DH, Luo C, Jian CD, Wu Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	MicroRNA Chronic Epilepsy miR-344a Epigenetics Apoptosis Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/0b8b3d143d2f4ae88af8dfa07d28c318
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spelling	oai:doaj.org-article:0b8b3d143d2f4ae88af8dfa07d28c3182021-12-02T00:29:52ZMicroRNA expression profiles in chronic epilepsy rats and neuroprotection from seizures by targeting miR-344a1178-2021https://doaj.org/article/0b8b3d143d2f4ae88af8dfa07d28c3182017-07-01T00:00:00Zhttps://www.dovepress.com/microrna-expression-profiles-in-chronic-epilepsy-rats-and-neuroprotect-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Xixia Liu,1,2 Yuhan Liao,1 Xiuxiu Wang,1 Donghua Zou,1 Chun Luo,1 Chongdong Jian,1 Yuan Wu1 1Department of Neurology, First Affiliated Hospital of Guangxi Medical University, 2Department of Rehabilitation, People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning, China Abstract: MicroRNA (miRNA) is believed to play a crucial role in the cause and treatment of epilepsy by controlling gene expression. However, it is still unclear how miRNA profiles change after multiple prolonged seizures and aggravation of brain injury in chronic epilepsy (CE). To investigate the role of miRNA in epilepsy, we utilized the CE rat models with pentylenetetrazol (PTZ) and miRNA profiles in the hippocampus. miRNA profiles were characterized using miRNA microarray analysis and were compared with the rats in the sham group, which received 0.9% physiological saline treatment at the same dose. Four up-regulated miRNAs (miR-139–3p, -770–5p, -127–5p, -331–3p) and 5 down-regulated miRNAs (miR-802–5p, -380–5p, -183–5p, -547–5p, -344a/-344a–5p) were found in the CE rats (fold change >1.5, P<0.05). Three of the dysregulated miRNAs were validated by quantitative real-time polymerase chain reaction, which revealed an outcome consistent with the initial results of the miRNA microarray analyses. Then, miR-344a agomir was intracerebroventricularly injected and followed by PTZ induction of CE models to investigate the effect of miR-344a in chronic neocortical epileptogenesis. After miRNA-344a agomir and scramble treatment, results showed a restoration of seizure behavior and a reduction in neuron damage in the cortex in miRNA-334a agomir treated rats. These data suggest that miRNA-344a might have a small modulatory effect on seizure-induced apoptosis signaling pathways in the cortex. Keywords: microRNA, chronic epilepsy, miR-344a, epigenetics, apoptosisLiu XXLiao YHWang XXZou DHLuo CJian CDWu YDove Medical PressarticleMicroRNAChronic EpilepsymiR-344aEpigeneticsApoptosisNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 13, Pp 2037-2044 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	MicroRNA Chronic Epilepsy miR-344a Epigenetics Apoptosis Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	MicroRNA Chronic Epilepsy miR-344a Epigenetics Apoptosis Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Liu XX Liao YH Wang XX Zou DH Luo C Jian CD Wu Y MicroRNA expression profiles in chronic epilepsy rats and neuroprotection from seizures by targeting miR-344a
description	Xixia Liu,1,2 Yuhan Liao,1 Xiuxiu Wang,1 Donghua Zou,1 Chun Luo,1 Chongdong Jian,1 Yuan Wu1 1Department of Neurology, First Affiliated Hospital of Guangxi Medical University, 2Department of Rehabilitation, People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning, China Abstract: MicroRNA (miRNA) is believed to play a crucial role in the cause and treatment of epilepsy by controlling gene expression. However, it is still unclear how miRNA profiles change after multiple prolonged seizures and aggravation of brain injury in chronic epilepsy (CE). To investigate the role of miRNA in epilepsy, we utilized the CE rat models with pentylenetetrazol (PTZ) and miRNA profiles in the hippocampus. miRNA profiles were characterized using miRNA microarray analysis and were compared with the rats in the sham group, which received 0.9% physiological saline treatment at the same dose. Four up-regulated miRNAs (miR-139–3p, -770–5p, -127–5p, -331–3p) and 5 down-regulated miRNAs (miR-802–5p, -380–5p, -183–5p, -547–5p, -344a/-344a–5p) were found in the CE rats (fold change >1.5, P<0.05). Three of the dysregulated miRNAs were validated by quantitative real-time polymerase chain reaction, which revealed an outcome consistent with the initial results of the miRNA microarray analyses. Then, miR-344a agomir was intracerebroventricularly injected and followed by PTZ induction of CE models to investigate the effect of miR-344a in chronic neocortical epileptogenesis. After miRNA-344a agomir and scramble treatment, results showed a restoration of seizure behavior and a reduction in neuron damage in the cortex in miRNA-334a agomir treated rats. These data suggest that miRNA-344a might have a small modulatory effect on seizure-induced apoptosis signaling pathways in the cortex. Keywords: microRNA, chronic epilepsy, miR-344a, epigenetics, apoptosis
format	article
author	Liu XX Liao YH Wang XX Zou DH Luo C Jian CD Wu Y
author_facet	Liu XX Liao YH Wang XX Zou DH Luo C Jian CD Wu Y
author_sort	Liu XX
title	MicroRNA expression profiles in chronic epilepsy rats and neuroprotection from seizures by targeting miR-344a
title_short	MicroRNA expression profiles in chronic epilepsy rats and neuroprotection from seizures by targeting miR-344a
title_full	MicroRNA expression profiles in chronic epilepsy rats and neuroprotection from seizures by targeting miR-344a
title_fullStr	MicroRNA expression profiles in chronic epilepsy rats and neuroprotection from seizures by targeting miR-344a
title_full_unstemmed	MicroRNA expression profiles in chronic epilepsy rats and neuroprotection from seizures by targeting miR-344a
title_sort	microrna expression profiles in chronic epilepsy rats and neuroprotection from seizures by targeting mir-344a
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/0b8b3d143d2f4ae88af8dfa07d28c318
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MicroRNA expression profiles in chronic epilepsy rats and neuroprotection from seizures by targeting miR-344a

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