Mitochondria change dynamics and morphology during grapevine leaf senescence.

Leaf senescence is the last stage of development of an organ and is aimed to its ordered disassembly and nutrient reallocation. Whereas chlorophyll gradually degrades during senescence in leaves, mitochondria need to maintain active to sustain the energy demands of senescing cells. Here we analysed...

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Autores principales:	Cristina Ruberti, Elisabetta Barizza, Martina Bodner, Nicoletta La Rocca, Roberto De Michele, Francesco Carimi, Fiorella Lo Schiavo, Michela Zottini
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0b8bdddb20fa46f6a7bf60a6c71f75e8
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spelling	oai:doaj.org-article:0b8bdddb20fa46f6a7bf60a6c71f75e82021-11-25T06:09:03ZMitochondria change dynamics and morphology during grapevine leaf senescence.1932-620310.1371/journal.pone.0102012https://doaj.org/article/0b8bdddb20fa46f6a7bf60a6c71f75e82014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25009991/?tool=EBIhttps://doaj.org/toc/1932-6203Leaf senescence is the last stage of development of an organ and is aimed to its ordered disassembly and nutrient reallocation. Whereas chlorophyll gradually degrades during senescence in leaves, mitochondria need to maintain active to sustain the energy demands of senescing cells. Here we analysed the motility and morphology of mitochondria in different stages of senescence in leaves of grapevine (Vitis vinifera), by stably expressing a GFP (green fluorescent protein) reporter targeted to these organelles. Results show that mitochondria were less dynamic and markedly changed morphology during senescence, passing from the elongated, branched structures found in mature leaves to enlarged and sparse organelles in senescent leaves. Progression of senescence in leaves was not synchronous, since changes in mitochondria from stomata were delayed. Mitochondrial morphology was also analysed in grapevine cell cultures. Mitochondria from cells at the end of their growth curve resembled those from senescing leaves, suggesting that cell cultures might represent a useful model system for senescence. Additionally, senescence-associated mitochondrial changes were observed in plants treated with high concentrations of cytokinins. Overall, morphology and dynamics of mitochondria might represent a reliable senescence marker for plant cells.Cristina RubertiElisabetta BarizzaMartina BodnerNicoletta La RoccaRoberto De MicheleFrancesco CarimiFiorella Lo SchiavoMichela ZottiniPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 7, p e102012 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cristina Ruberti Elisabetta Barizza Martina Bodner Nicoletta La Rocca Roberto De Michele Francesco Carimi Fiorella Lo Schiavo Michela Zottini Mitochondria change dynamics and morphology during grapevine leaf senescence.
description	Leaf senescence is the last stage of development of an organ and is aimed to its ordered disassembly and nutrient reallocation. Whereas chlorophyll gradually degrades during senescence in leaves, mitochondria need to maintain active to sustain the energy demands of senescing cells. Here we analysed the motility and morphology of mitochondria in different stages of senescence in leaves of grapevine (Vitis vinifera), by stably expressing a GFP (green fluorescent protein) reporter targeted to these organelles. Results show that mitochondria were less dynamic and markedly changed morphology during senescence, passing from the elongated, branched structures found in mature leaves to enlarged and sparse organelles in senescent leaves. Progression of senescence in leaves was not synchronous, since changes in mitochondria from stomata were delayed. Mitochondrial morphology was also analysed in grapevine cell cultures. Mitochondria from cells at the end of their growth curve resembled those from senescing leaves, suggesting that cell cultures might represent a useful model system for senescence. Additionally, senescence-associated mitochondrial changes were observed in plants treated with high concentrations of cytokinins. Overall, morphology and dynamics of mitochondria might represent a reliable senescence marker for plant cells.
format	article
author	Cristina Ruberti Elisabetta Barizza Martina Bodner Nicoletta La Rocca Roberto De Michele Francesco Carimi Fiorella Lo Schiavo Michela Zottini
author_facet	Cristina Ruberti Elisabetta Barizza Martina Bodner Nicoletta La Rocca Roberto De Michele Francesco Carimi Fiorella Lo Schiavo Michela Zottini
author_sort	Cristina Ruberti
title	Mitochondria change dynamics and morphology during grapevine leaf senescence.
title_short	Mitochondria change dynamics and morphology during grapevine leaf senescence.
title_full	Mitochondria change dynamics and morphology during grapevine leaf senescence.
title_fullStr	Mitochondria change dynamics and morphology during grapevine leaf senescence.
title_full_unstemmed	Mitochondria change dynamics and morphology during grapevine leaf senescence.
title_sort	mitochondria change dynamics and morphology during grapevine leaf senescence.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/0b8bdddb20fa46f6a7bf60a6c71f75e8
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_version_	1718414085025955840

Mitochondria change dynamics and morphology during grapevine leaf senescence.

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