Correlation between <i>mazEF</i> Toxin-Antitoxin System Expression and Methicillin Susceptibility in <i>Staphylococcus aureus</i> and Its Relation to Biofilm-Formation
Methicillin resistance in <i>Staphylococcus aureus</i> has become prevalent globally. Moreover, biofilm-formation makes it more difficult to eradicate bacteria by antibiotics. The <i>mazEF</i> toxin-antitoxin system encodes for mazF, which acts as an endoribonuclease that cle...
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| Autores principales: | , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/0b9206a6c2ad4d5fa72b38c072200fea |
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| Sumario: | Methicillin resistance in <i>Staphylococcus aureus</i> has become prevalent globally. Moreover, biofilm-formation makes it more difficult to eradicate bacteria by antibiotics. The <i>mazEF</i> toxin-antitoxin system encodes for mazF, which acts as an endoribonuclease that cleaves cellular mRNAs at specific sequence motifs (ACA), and mazE, which opposes the mazF action. Our goal was to detect <i>mazEF</i> expression in methicillin-resistant <i>S. aureus</i> (MRSA) isolates compared with methicillin-sensitive <i>S. aureus</i> (MSSA) isolates and determine its relation to methicillin susceptibility as well as biofilm-formation. According to their susceptibility to cefoxitin disks, 100 <i>S. aureus</i> isolates obtained from patients admitted to Cairo University Hospitals were categorized into 50 MSSA and 50 MRSA according to their susceptibility to cefoxitin disks (30 µg). Antimicrobial susceptibility and biofilm-formation were investigated using the disk diffusion method and tissue culture plate method, respectively. Finally, using real-time PCR, <i>mazEF</i> expression was estimated and correlated to methicillin susceptibility and biofilm formation. Both MRSA and MSSA isolates showed the best sensitivity results with linezolid and gentamicin, where about 88% of MRSA isolates and 96% of MSSA isolates were sensitive to linezolid while 76% of MRSA isolates and 84% of MSSA isolates were sensitive to gentamicin. MRSA isolates were significantly more able to form biofilm than MSSA isolates (<i>p</i>-value = 0.037). The <i>mazEF</i> expression was significantly correlated to methicillin resistance in <i>S. aureus</i> (<i>p</i>-value < 0.001), but not to biofilm-formation. |
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