Correlation between <i>mazEF</i> Toxin-Antitoxin System Expression and Methicillin Susceptibility in <i>Staphylococcus aureus</i> and Its Relation to Biofilm-Formation

Correlation between <i>mazEF</i> Toxin-Antitoxin System Expression and Methicillin Susceptibility in <i>Staphylococcus aureus</i> and Its Relation to Biofilm-Formation

Methicillin resistance in <i>Staphylococcus aureus</i> has become prevalent globally. Moreover, biofilm-formation makes it more difficult to eradicate bacteria by antibiotics. The <i>mazEF</i> toxin-antitoxin system encodes for mazF, which acts as an endoribonuclease that cle...

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Autores principales: Aya Abd El rahman, Yasmine El kholy, Rania Y. Shash
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
Materias:
MRSA
MSSA
toxin-antitoxin systems
<i>mazEF</i>
biofilm
Biology (General)
QH301-705.5
Acceso en línea:https://doaj.org/article/0b9206a6c2ad4d5fa72b38c072200fea
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spelling oai:doaj.org-article:0b9206a6c2ad4d5fa72b38c072200fea2021-11-25T18:24:46ZCorrelation between <i>mazEF</i> Toxin-Antitoxin System Expression and Methicillin Susceptibility in <i>Staphylococcus aureus</i> and Its Relation to Biofilm-Formation10.3390/microorganisms91122742076-2607https://doaj.org/article/0b9206a6c2ad4d5fa72b38c072200fea2021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2274https://doaj.org/toc/2076-2607Methicillin resistance in <i>Staphylococcus aureus</i> has become prevalent globally. Moreover, biofilm-formation makes it more difficult to eradicate bacteria by antibiotics. The <i>mazEF</i> toxin-antitoxin system encodes for mazF, which acts as an endoribonuclease that cleaves cellular mRNAs at specific sequence motifs (ACA), and mazE, which opposes the mazF action. Our goal was to detect <i>mazEF</i> expression in methicillin-resistant <i>S. aureus</i> (MRSA) isolates compared with methicillin-sensitive <i>S. aureus</i> (MSSA) isolates and determine its relation to methicillin susceptibility as well as biofilm-formation. According to their susceptibility to cefoxitin disks, 100 <i>S. aureus</i> isolates obtained from patients admitted to Cairo University Hospitals were categorized into 50 MSSA and 50 MRSA according to their susceptibility to cefoxitin disks (30 µg). Antimicrobial susceptibility and biofilm-formation were investigated using the disk diffusion method and tissue culture plate method, respectively. Finally, using real-time PCR, <i>mazEF</i> expression was estimated and correlated to methicillin susceptibility and biofilm formation. Both MRSA and MSSA isolates showed the best sensitivity results with linezolid and gentamicin, where about 88% of MRSA isolates and 96% of MSSA isolates were sensitive to linezolid while 76% of MRSA isolates and 84% of MSSA isolates were sensitive to gentamicin. MRSA isolates were significantly more able to form biofilm than MSSA isolates (<i>p</i>-value = 0.037). The <i>mazEF</i> expression was significantly correlated to methicillin resistance in <i>S. aureus</i> (<i>p</i>-value < 0.001), but not to biofilm-formation.Aya Abd El rahmanYasmine El kholyRania Y. ShashMDPI AGarticleMRSAMSSAtoxin-antitoxin systems<i>mazEF</i>biofilmBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2274, p 2274 (2021)
institution DOAJ
collection DOAJ
language EN
topic MRSA
MSSA
toxin-antitoxin systems
<i>mazEF</i>
biofilm
Biology (General)
QH301-705.5
spellingShingle MRSA
MSSA
toxin-antitoxin systems
<i>mazEF</i>
biofilm
Biology (General)
QH301-705.5
Aya Abd El rahman
Yasmine El kholy
Rania Y. Shash
Correlation between <i>mazEF</i> Toxin-Antitoxin System Expression and Methicillin Susceptibility in <i>Staphylococcus aureus</i> and Its Relation to Biofilm-Formation
description Methicillin resistance in <i>Staphylococcus aureus</i> has become prevalent globally. Moreover, biofilm-formation makes it more difficult to eradicate bacteria by antibiotics. The <i>mazEF</i> toxin-antitoxin system encodes for mazF, which acts as an endoribonuclease that cleaves cellular mRNAs at specific sequence motifs (ACA), and mazE, which opposes the mazF action. Our goal was to detect <i>mazEF</i> expression in methicillin-resistant <i>S. aureus</i> (MRSA) isolates compared with methicillin-sensitive <i>S. aureus</i> (MSSA) isolates and determine its relation to methicillin susceptibility as well as biofilm-formation. According to their susceptibility to cefoxitin disks, 100 <i>S. aureus</i> isolates obtained from patients admitted to Cairo University Hospitals were categorized into 50 MSSA and 50 MRSA according to their susceptibility to cefoxitin disks (30 µg). Antimicrobial susceptibility and biofilm-formation were investigated using the disk diffusion method and tissue culture plate method, respectively. Finally, using real-time PCR, <i>mazEF</i> expression was estimated and correlated to methicillin susceptibility and biofilm formation. Both MRSA and MSSA isolates showed the best sensitivity results with linezolid and gentamicin, where about 88% of MRSA isolates and 96% of MSSA isolates were sensitive to linezolid while 76% of MRSA isolates and 84% of MSSA isolates were sensitive to gentamicin. MRSA isolates were significantly more able to form biofilm than MSSA isolates (<i>p</i>-value = 0.037). The <i>mazEF</i> expression was significantly correlated to methicillin resistance in <i>S. aureus</i> (<i>p</i>-value < 0.001), but not to biofilm-formation.
format article
author Aya Abd El rahman
Yasmine El kholy
Rania Y. Shash
author_facet Aya Abd El rahman
Yasmine El kholy
Rania Y. Shash
author_sort Aya Abd El rahman
title Correlation between <i>mazEF</i> Toxin-Antitoxin System Expression and Methicillin Susceptibility in <i>Staphylococcus aureus</i> and Its Relation to Biofilm-Formation
title_short Correlation between <i>mazEF</i> Toxin-Antitoxin System Expression and Methicillin Susceptibility in <i>Staphylococcus aureus</i> and Its Relation to Biofilm-Formation
title_full Correlation between <i>mazEF</i> Toxin-Antitoxin System Expression and Methicillin Susceptibility in <i>Staphylococcus aureus</i> and Its Relation to Biofilm-Formation
title_fullStr Correlation between <i>mazEF</i> Toxin-Antitoxin System Expression and Methicillin Susceptibility in <i>Staphylococcus aureus</i> and Its Relation to Biofilm-Formation
title_full_unstemmed Correlation between <i>mazEF</i> Toxin-Antitoxin System Expression and Methicillin Susceptibility in <i>Staphylococcus aureus</i> and Its Relation to Biofilm-Formation
title_sort correlation between <i>mazef</i> toxin-antitoxin system expression and methicillin susceptibility in <i>staphylococcus aureus</i> and its relation to biofilm-formation
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/0b9206a6c2ad4d5fa72b38c072200fea
work_keys_str_mv AT ayaabdelrahman correlationbetweenimazefitoxinantitoxinsystemexpressionandmethicillinsusceptibilityinistaphylococcusaureusianditsrelationtobiofilmformation
AT yasmineelkholy correlationbetweenimazefitoxinantitoxinsystemexpressionandmethicillinsusceptibilityinistaphylococcusaureusianditsrelationtobiofilmformation
AT raniayshash correlationbetweenimazefitoxinantitoxinsystemexpressionandmethicillinsusceptibilityinistaphylococcusaureusianditsrelationtobiofilmformation
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