Two-Target Quantitative PCR To Predict Library Composition for Shallow Shotgun Sequencing
When determining human microbiota composition, shotgun sequencing is a powerful tool that can generate large amounts of data. However, in sample compositions with low or variable microbial density, shallowing sequencing can negatively affect resulting data.
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Autores principales: | , , , , , , , , , |
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Formato: | article |
Lenguaje: | EN |
Publicado: |
American Society for Microbiology
2021
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Materias: | |
Acceso en línea: | https://doaj.org/article/0b99f69fb2d04cf0bf59b41b8420466b |
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Sumario: | When determining human microbiota composition, shotgun sequencing is a powerful tool that can generate large amounts of data. However, in sample compositions with low or variable microbial density, shallowing sequencing can negatively affect resulting data. |
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