Two-Target Quantitative PCR To Predict Library Composition for Shallow Shotgun Sequencing

When determining human microbiota composition, shotgun sequencing is a powerful tool that can generate large amounts of data. However, in sample compositions with low or variable microbial density, shallowing sequencing can negatively affect resulting data.

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Autores principales:	Matthew Y. Cho, Marc Oliva, Anna Spreafico, Bo Chen, Xu Wei, Yoojin Choi, Rupert Kaul, Lillian L. Siu, Bryan Coburn, Pierre H. H. Schneeberger
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2021
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/0b99f69fb2d04cf0bf59b41b8420466b
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spelling	oai:doaj.org-article:0b99f69fb2d04cf0bf59b41b8420466b2021-12-02T19:35:57ZTwo-Target Quantitative PCR To Predict Library Composition for Shallow Shotgun Sequencing2379-507710.1128/mSystems.00552-21https://doaj.org/article/0b99f69fb2d04cf0bf59b41b8420466b2021-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00552-21https://doaj.org/toc/2379-5077 When determining human microbiota composition, shotgun sequencing is a powerful tool that can generate large amounts of data. However, in sample compositions with low or variable microbial density, shallowing sequencing can negatively affect resulting data.Matthew Y. ChoMarc OlivaAnna SpreaficoBo ChenXu WeiYoojin ChoiRupert KaulLillian L. SiuBryan CoburnPierre H. H. SchneebergerAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmSystems, Vol 6, Iss 4 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Matthew Y. Cho Marc Oliva Anna Spreafico Bo Chen Xu Wei Yoojin Choi Rupert Kaul Lillian L. Siu Bryan Coburn Pierre H. H. Schneeberger Two-Target Quantitative PCR To Predict Library Composition for Shallow Shotgun Sequencing
description	When determining human microbiota composition, shotgun sequencing is a powerful tool that can generate large amounts of data. However, in sample compositions with low or variable microbial density, shallowing sequencing can negatively affect resulting data.
format	article
author	Matthew Y. Cho Marc Oliva Anna Spreafico Bo Chen Xu Wei Yoojin Choi Rupert Kaul Lillian L. Siu Bryan Coburn Pierre H. H. Schneeberger
author_facet	Matthew Y. Cho Marc Oliva Anna Spreafico Bo Chen Xu Wei Yoojin Choi Rupert Kaul Lillian L. Siu Bryan Coburn Pierre H. H. Schneeberger
author_sort	Matthew Y. Cho
title	Two-Target Quantitative PCR To Predict Library Composition for Shallow Shotgun Sequencing
title_short	Two-Target Quantitative PCR To Predict Library Composition for Shallow Shotgun Sequencing
title_full	Two-Target Quantitative PCR To Predict Library Composition for Shallow Shotgun Sequencing
title_fullStr	Two-Target Quantitative PCR To Predict Library Composition for Shallow Shotgun Sequencing
title_full_unstemmed	Two-Target Quantitative PCR To Predict Library Composition for Shallow Shotgun Sequencing
title_sort	two-target quantitative pcr to predict library composition for shallow shotgun sequencing
publisher	American Society for Microbiology
publishDate	2021
url	https://doaj.org/article/0b99f69fb2d04cf0bf59b41b8420466b
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Two-Target Quantitative PCR To Predict Library Composition for Shallow Shotgun Sequencing

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