Two-Target Quantitative PCR To Predict Library Composition for Shallow Shotgun Sequencing

Two-Target Quantitative PCR To Predict Library Composition for Shallow Shotgun Sequencing

When determining human microbiota composition, shotgun sequencing is a powerful tool that can generate large amounts of data. However, in sample compositions with low or variable microbial density, shallowing sequencing can negatively affect resulting data.

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Autores principales: Matthew Y. Cho, Marc Oliva, Anna Spreafico, Bo Chen, Xu Wei, Yoojin Choi, Rupert Kaul, Lillian L. Siu, Bryan Coburn, Pierre H. H. Schneeberger
Formato: article
Lenguaje:EN
Publicado: American Society for Microbiology 2021
Materias:
Microbiology
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Acceso en línea:https://doaj.org/article/0b99f69fb2d04cf0bf59b41b8420466b
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