The transcriptomics of glucocorticoid receptor signaling in developing zebrafish.

Cortisol is the primary corticosteroid in teleosts that is released in response to stressor activation of the hypothalamus-pituitary-interrenal axis. The target tissue action of this hormone is primarily mediated by the intracellular glucocorticoid receptor (GR), a ligand-bound transcription factor....

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Autores principales:	Dinushan Nesan, Mathilakath M Vijayan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0b9b8b2a87b94dbdaee964985aab1e9a
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spelling	oai:doaj.org-article:0b9b8b2a87b94dbdaee964985aab1e9a2021-11-18T08:45:20ZThe transcriptomics of glucocorticoid receptor signaling in developing zebrafish.1932-620310.1371/journal.pone.0080726https://doaj.org/article/0b9b8b2a87b94dbdaee964985aab1e9a2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24348914/?tool=EBIhttps://doaj.org/toc/1932-6203Cortisol is the primary corticosteroid in teleosts that is released in response to stressor activation of the hypothalamus-pituitary-interrenal axis. The target tissue action of this hormone is primarily mediated by the intracellular glucocorticoid receptor (GR), a ligand-bound transcription factor. In developing zebrafish (Danio rerio) embryos, GR transcripts and cortisol are maternally deposited into the oocyte prior to fertilization and influence early embryogenesis. To better understand of the molecular mechanisms involved, we investigated changes in the developmental transcriptome prior to hatch, in response to morpholino oligonucleotide knockdown of GR using the Agilent zebrafish microarray platform. A total of 1313 and 836 mRNA transcripts were significantly changed at 24 and 36 hours post fertilization (hpf), respectively. Functional analysis revealed numerous developmental processes under GR regulation, including neurogenesis, eye development, skeletal and cardiac muscle formation. Together, this study underscores a critical role for glucocorticoid signaling in programming molecular events essential for zebrafish development.Dinushan NesanMathilakath M VijayanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e80726 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dinushan Nesan Mathilakath M Vijayan The transcriptomics of glucocorticoid receptor signaling in developing zebrafish.
description	Cortisol is the primary corticosteroid in teleosts that is released in response to stressor activation of the hypothalamus-pituitary-interrenal axis. The target tissue action of this hormone is primarily mediated by the intracellular glucocorticoid receptor (GR), a ligand-bound transcription factor. In developing zebrafish (Danio rerio) embryos, GR transcripts and cortisol are maternally deposited into the oocyte prior to fertilization and influence early embryogenesis. To better understand of the molecular mechanisms involved, we investigated changes in the developmental transcriptome prior to hatch, in response to morpholino oligonucleotide knockdown of GR using the Agilent zebrafish microarray platform. A total of 1313 and 836 mRNA transcripts were significantly changed at 24 and 36 hours post fertilization (hpf), respectively. Functional analysis revealed numerous developmental processes under GR regulation, including neurogenesis, eye development, skeletal and cardiac muscle formation. Together, this study underscores a critical role for glucocorticoid signaling in programming molecular events essential for zebrafish development.
format	article
author	Dinushan Nesan Mathilakath M Vijayan
author_facet	Dinushan Nesan Mathilakath M Vijayan
author_sort	Dinushan Nesan
title	The transcriptomics of glucocorticoid receptor signaling in developing zebrafish.
title_short	The transcriptomics of glucocorticoid receptor signaling in developing zebrafish.
title_full	The transcriptomics of glucocorticoid receptor signaling in developing zebrafish.
title_fullStr	The transcriptomics of glucocorticoid receptor signaling in developing zebrafish.
title_full_unstemmed	The transcriptomics of glucocorticoid receptor signaling in developing zebrafish.
title_sort	transcriptomics of glucocorticoid receptor signaling in developing zebrafish.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/0b9b8b2a87b94dbdaee964985aab1e9a
work_keys_str_mv	AT dinushannesan thetranscriptomicsofglucocorticoidreceptorsignalingindevelopingzebrafish AT mathilakathmvijayan thetranscriptomicsofglucocorticoidreceptorsignalingindevelopingzebrafish AT dinushannesan transcriptomicsofglucocorticoidreceptorsignalingindevelopingzebrafish AT mathilakathmvijayan transcriptomicsofglucocorticoidreceptorsignalingindevelopingzebrafish
_version_	1718421305763561472

The transcriptomics of glucocorticoid receptor signaling in developing zebrafish.

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