Morphology of a fibrin nanocoating influences dermal fibroblast behavior

Julia Pajorova,1 Marketa Bacakova,1 Jana Musilkova,1 Antonin Broz,1 Daniel Hadraba,1,2 Frantisek Lopot,2 Lucie Bacakova1 1Department of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic; 2Department of Anatomy and Biomechanics, Fac...

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Autores principales:	Pajorova J, Bacakova M, Musilkova J, Broz A, Hadraba D, Lopot F, Bacakova L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	nanofibers fibrin nanocoating dermal fibroblasts extracellular matrix synthesis Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0b9cc58e168f41bd89589a3393595ad2
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spelling	oai:doaj.org-article:0b9cc58e168f41bd89589a3393595ad22021-12-02T07:07:09ZMorphology of a fibrin nanocoating influences dermal fibroblast behavior1178-2013https://doaj.org/article/0b9cc58e168f41bd89589a3393595ad22018-06-01T00:00:00Zhttps://www.dovepress.com/morphology-of-a-fibrin-nanocoating-influences-dermal-fibroblast-behavi-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Julia Pajorova,1 Marketa Bacakova,1 Jana Musilkova,1 Antonin Broz,1 Daniel Hadraba,1,2 Frantisek Lopot,2 Lucie Bacakova1 1Department of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic; 2Department of Anatomy and Biomechanics, Faculty of Physical Education and Sport, Charles University, Prague, Czech Republic Background: Our study focuses on the fabrication of appropriate scaffolds for skin wound healing. This research brings valuable insights into the molecular mechanisms of adhesion, proliferation, and control of cell behavior through the extracellular matrix represented by synthetic biodegradable nanofibrous membranes coated by biomolecules. Methods: Nanofibrous polylactic acid (PLA) membranes were prepared by a needle-less electrospinning technology. These membranes were coated with fibrin according to two preparation protocols, and additionally they were coated with fibronectin in order to increase the cell affinity for colonizing the PLA membranes. The adhesion, growth, and extracellular matrix protein production of neonatal human dermal fibroblasts were evaluated on the nanofibrous membranes. Results: Our results showed that fibrin-coated membranes improved the adhesion and proliferation of human dermal fibroblasts. The morphology of the fibrin nanocoating seems to be crucial for the adhesion of fibroblasts, and consequently for their phenotypic maturation. Fibrin either covered the individual fibers in the membrane (F1 nanocoating), or covered the individual fibers and also formed a fine homogeneous nanofibrous mesh on the surface of the membrane (F2 nanocoating), depending on the mode of fibrin preparation. The fibroblasts on the membranes with the F1 nanocoating remained in their typical spindle-like shape. However, the cells on the F2 nanocoating were spread mostly in a polygon-like shape, and their proliferation was significantly higher. Fibronectin formed an additional mesh attached to the surface of the fibrin mesh, and further enhanced the cell adhesion and growth. The relative gene expression and protein production of collagen I and fibronectin were higher on the F2 nanocoating than on the F1 nanocoating. Conclusion: A PLA membrane coated with a homogeneous fibrin mesh seems to be promising for the construction of temporary full-thickness skin tissue substitutes. Keywords: nanofibers, fibrin, nanocoating, dermal fibroblasts, extracellular matrix synthesis, skin substitute, polylactic acidPajorova JBacakova MMusilkova JBroz AHadraba DLopot FBacakova LDove Medical Pressarticlenanofibersfibrinnanocoatingdermal fibroblastsextracellular matrix synthesisMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 3367-3380 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	nanofibers fibrin nanocoating dermal fibroblasts extracellular matrix synthesis Medicine (General) R5-920
spellingShingle	nanofibers fibrin nanocoating dermal fibroblasts extracellular matrix synthesis Medicine (General) R5-920 Pajorova J Bacakova M Musilkova J Broz A Hadraba D Lopot F Bacakova L Morphology of a fibrin nanocoating influences dermal fibroblast behavior
description	Julia Pajorova,1 Marketa Bacakova,1 Jana Musilkova,1 Antonin Broz,1 Daniel Hadraba,1,2 Frantisek Lopot,2 Lucie Bacakova1 1Department of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic; 2Department of Anatomy and Biomechanics, Faculty of Physical Education and Sport, Charles University, Prague, Czech Republic Background: Our study focuses on the fabrication of appropriate scaffolds for skin wound healing. This research brings valuable insights into the molecular mechanisms of adhesion, proliferation, and control of cell behavior through the extracellular matrix represented by synthetic biodegradable nanofibrous membranes coated by biomolecules. Methods: Nanofibrous polylactic acid (PLA) membranes were prepared by a needle-less electrospinning technology. These membranes were coated with fibrin according to two preparation protocols, and additionally they were coated with fibronectin in order to increase the cell affinity for colonizing the PLA membranes. The adhesion, growth, and extracellular matrix protein production of neonatal human dermal fibroblasts were evaluated on the nanofibrous membranes. Results: Our results showed that fibrin-coated membranes improved the adhesion and proliferation of human dermal fibroblasts. The morphology of the fibrin nanocoating seems to be crucial for the adhesion of fibroblasts, and consequently for their phenotypic maturation. Fibrin either covered the individual fibers in the membrane (F1 nanocoating), or covered the individual fibers and also formed a fine homogeneous nanofibrous mesh on the surface of the membrane (F2 nanocoating), depending on the mode of fibrin preparation. The fibroblasts on the membranes with the F1 nanocoating remained in their typical spindle-like shape. However, the cells on the F2 nanocoating were spread mostly in a polygon-like shape, and their proliferation was significantly higher. Fibronectin formed an additional mesh attached to the surface of the fibrin mesh, and further enhanced the cell adhesion and growth. The relative gene expression and protein production of collagen I and fibronectin were higher on the F2 nanocoating than on the F1 nanocoating. Conclusion: A PLA membrane coated with a homogeneous fibrin mesh seems to be promising for the construction of temporary full-thickness skin tissue substitutes. Keywords: nanofibers, fibrin, nanocoating, dermal fibroblasts, extracellular matrix synthesis, skin substitute, polylactic acid
format	article
author	Pajorova J Bacakova M Musilkova J Broz A Hadraba D Lopot F Bacakova L
author_facet	Pajorova J Bacakova M Musilkova J Broz A Hadraba D Lopot F Bacakova L
author_sort	Pajorova J
title	Morphology of a fibrin nanocoating influences dermal fibroblast behavior
title_short	Morphology of a fibrin nanocoating influences dermal fibroblast behavior
title_full	Morphology of a fibrin nanocoating influences dermal fibroblast behavior
title_fullStr	Morphology of a fibrin nanocoating influences dermal fibroblast behavior
title_full_unstemmed	Morphology of a fibrin nanocoating influences dermal fibroblast behavior
title_sort	morphology of a fibrin nanocoating influences dermal fibroblast behavior
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/0b9cc58e168f41bd89589a3393595ad2
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_version_	1718399556381573120

Morphology of a fibrin nanocoating influences dermal fibroblast behavior

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