Nanobody-derived nanobiotechnology tool kits for diverse biomedical and biotechnology applications

Yongzhong Wang,1 Zhen Fan,2 Lei Shao,3 Xiaowei Kong,1 Xianjuan Hou,1 Dongrui Tian,1 Ying Sun,1 Yazhong Xiao,1 Li Yu4 1School of Life Sciences, Collaborative Innovation Center of Modern Bio-manufacture, Anhui University, Hefei, People’s Republic of China; 2Department of Biomedical Engineer...

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Autores principales:	Wang Y, Fan Z, Shao L, Kong X, Hou X, Tian D, Sun Y, Xiao Y, Yu L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Nanobody VHH V-NAR HCAbs IgNAR Nanobiotechnology Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0b9fa51c58154f26b711a62e1309d61d
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spelling	oai:doaj.org-article:0b9fa51c58154f26b711a62e1309d61d2021-12-02T07:15:14ZNanobody-derived nanobiotechnology tool kits for diverse biomedical and biotechnology applications1178-2013https://doaj.org/article/0b9fa51c58154f26b711a62e1309d61d2016-07-01T00:00:00Zhttps://www.dovepress.com/nanobody-derived-nanobiotechnology-tool-kits-for-diverse-biomedical-an-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yongzhong Wang,1 Zhen Fan,2 Lei Shao,3 Xiaowei Kong,1 Xianjuan Hou,1 Dongrui Tian,1 Ying Sun,1 Yazhong Xiao,1 Li Yu4 1School of Life Sciences, Collaborative Innovation Center of Modern Bio-manufacture, Anhui University, Hefei, People’s Republic of China; 2Department of Biomedical Engineering, The Ohio State University, Columbus, OH, USA; 3State Key Laboratory of New Drugs and Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, Shanghai, 4Department of Microbiology and Parasitology, Anhui Provincial Laboratory of Microbiology and Parasitology, Anhui Key Laboratory of Zoonoses, Anhui Medical University, Hefei, People’s Republic of China Abstract: Owing to peculiar properties of nanobody, including nanoscale size, robust structure, stable and soluble behaviors in aqueous solution, reversible refolding, high affinity and specificity for only one cognate target, superior cryptic cleft accessibility, and deep tissue penetration, as well as a sustainable source, it has been an ideal research tool for the development of sophisticated nanobiotechnologies. Currently, the nanobody has been evolved into versatile research and application tool kits for diverse biomedical and biotechnology applications. Various nanobody-derived formats, including the nanobody itself, the radionuclide or fluorescent-labeled nanobodies, nanobody homo- or heteromultimers, nanobody-coated nanoparticles, and nanobody-displayed bacteriophages, have been successfully demonstrated as powerful nanobiotechnological tool kits for basic biomedical research, targeting drug delivery and therapy, disease diagnosis, bioimaging, and agricultural and plant protection. These applications indicate a special advantage of these nanobody-derived technologies, already surpassing the “me-too” products of other equivalent binders, such as the full-length antibodies, single-chain variable fragments, antigen-binding fragments, targeting peptides, and DNA-based aptamers. In this review, we summarize the current state of the art in nanobody research, focusing on the nanobody structural features, nanobody production approach, nanobody-derived nanobiotechnology tool kits, and the potentially diverse applications in biomedicine and biotechnology. The future trends, challenges, and limitations of the nanobody-derived nanobiotechnology tool kits are also discussed. Keywords: nanobody, VHH, V-NAR, HCAbs, IgNAR, nanobiotechnologyWang YFan ZShao LKong XHou XTian DSun YXiao YYu LDove Medical PressarticleNanobodyVHHV-NARHCAbsIgNARNanobiotechnologyMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 3287-3303 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Nanobody VHH V-NAR HCAbs IgNAR Nanobiotechnology Medicine (General) R5-920
spellingShingle	Nanobody VHH V-NAR HCAbs IgNAR Nanobiotechnology Medicine (General) R5-920 Wang Y Fan Z Shao L Kong X Hou X Tian D Sun Y Xiao Y Yu L Nanobody-derived nanobiotechnology tool kits for diverse biomedical and biotechnology applications
description	Yongzhong Wang,1 Zhen Fan,2 Lei Shao,3 Xiaowei Kong,1 Xianjuan Hou,1 Dongrui Tian,1 Ying Sun,1 Yazhong Xiao,1 Li Yu4 1School of Life Sciences, Collaborative Innovation Center of Modern Bio-manufacture, Anhui University, Hefei, People’s Republic of China; 2Department of Biomedical Engineering, The Ohio State University, Columbus, OH, USA; 3State Key Laboratory of New Drugs and Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, Shanghai, 4Department of Microbiology and Parasitology, Anhui Provincial Laboratory of Microbiology and Parasitology, Anhui Key Laboratory of Zoonoses, Anhui Medical University, Hefei, People’s Republic of China Abstract: Owing to peculiar properties of nanobody, including nanoscale size, robust structure, stable and soluble behaviors in aqueous solution, reversible refolding, high affinity and specificity for only one cognate target, superior cryptic cleft accessibility, and deep tissue penetration, as well as a sustainable source, it has been an ideal research tool for the development of sophisticated nanobiotechnologies. Currently, the nanobody has been evolved into versatile research and application tool kits for diverse biomedical and biotechnology applications. Various nanobody-derived formats, including the nanobody itself, the radionuclide or fluorescent-labeled nanobodies, nanobody homo- or heteromultimers, nanobody-coated nanoparticles, and nanobody-displayed bacteriophages, have been successfully demonstrated as powerful nanobiotechnological tool kits for basic biomedical research, targeting drug delivery and therapy, disease diagnosis, bioimaging, and agricultural and plant protection. These applications indicate a special advantage of these nanobody-derived technologies, already surpassing the “me-too” products of other equivalent binders, such as the full-length antibodies, single-chain variable fragments, antigen-binding fragments, targeting peptides, and DNA-based aptamers. In this review, we summarize the current state of the art in nanobody research, focusing on the nanobody structural features, nanobody production approach, nanobody-derived nanobiotechnology tool kits, and the potentially diverse applications in biomedicine and biotechnology. The future trends, challenges, and limitations of the nanobody-derived nanobiotechnology tool kits are also discussed. Keywords: nanobody, VHH, V-NAR, HCAbs, IgNAR, nanobiotechnology
format	article
author	Wang Y Fan Z Shao L Kong X Hou X Tian D Sun Y Xiao Y Yu L
author_facet	Wang Y Fan Z Shao L Kong X Hou X Tian D Sun Y Xiao Y Yu L
author_sort	Wang Y
title	Nanobody-derived nanobiotechnology tool kits for diverse biomedical and biotechnology applications
title_short	Nanobody-derived nanobiotechnology tool kits for diverse biomedical and biotechnology applications
title_full	Nanobody-derived nanobiotechnology tool kits for diverse biomedical and biotechnology applications
title_fullStr	Nanobody-derived nanobiotechnology tool kits for diverse biomedical and biotechnology applications
title_full_unstemmed	Nanobody-derived nanobiotechnology tool kits for diverse biomedical and biotechnology applications
title_sort	nanobody-derived nanobiotechnology tool kits for diverse biomedical and biotechnology applications
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/0b9fa51c58154f26b711a62e1309d61d
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Nanobody-derived nanobiotechnology tool kits for diverse biomedical and biotechnology applications

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