Integrated metagenomics/metaproteomics reveals human host-microbiota signatures of Crohn's disease.

Crohn's disease (CD) is an inflammatory bowel disease of complex etiology, although dysbiosis of the gut microbiota has been implicated in chronic immune-mediated inflammation associated with CD. Here we combined shotgun metagenomic and metaproteomic approaches to identify potential functional...

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Autores principales:	Alison R Erickson, Brandi L Cantarel, Regina Lamendella, Youssef Darzi, Emmanuel F Mongodin, Chongle Pan, Manesh Shah, Jonas Halfvarson, Curt Tysk, Bernard Henrissat, Jeroen Raes, Nathan C Verberkmoes, Claire M Fraser, Robert L Hettich, Janet K Jansson
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0ba838e8eed447ac81829dfa7897d160
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spelling	oai:doaj.org-article:0ba838e8eed447ac81829dfa7897d1602021-11-18T08:07:19ZIntegrated metagenomics/metaproteomics reveals human host-microbiota signatures of Crohn's disease.1932-620310.1371/journal.pone.0049138https://doaj.org/article/0ba838e8eed447ac81829dfa7897d1602012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23209564/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Crohn's disease (CD) is an inflammatory bowel disease of complex etiology, although dysbiosis of the gut microbiota has been implicated in chronic immune-mediated inflammation associated with CD. Here we combined shotgun metagenomic and metaproteomic approaches to identify potential functional signatures of CD in stool samples from six twin pairs that were either healthy, or that had CD in the ileum (ICD) or colon (CCD). Integration of these omics approaches revealed several genes, proteins, and pathways that primarily differentiated ICD from healthy subjects, including depletion of many proteins in ICD. In addition, the ICD phenotype was associated with alterations in bacterial carbohydrate metabolism, bacterial-host interactions, as well as human host-secreted enzymes. This eco-systems biology approach underscores the link between the gut microbiota and functional alterations in the pathophysiology of Crohn's disease and aids in identification of novel diagnostic targets and disease specific biomarkers.Alison R EricksonBrandi L CantarelRegina LamendellaYoussef DarziEmmanuel F MongodinChongle PanManesh ShahJonas HalfvarsonCurt TyskBernard HenrissatJeroen RaesNathan C VerberkmoesClaire M FraserRobert L HettichJanet K JanssonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e49138 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alison R Erickson Brandi L Cantarel Regina Lamendella Youssef Darzi Emmanuel F Mongodin Chongle Pan Manesh Shah Jonas Halfvarson Curt Tysk Bernard Henrissat Jeroen Raes Nathan C Verberkmoes Claire M Fraser Robert L Hettich Janet K Jansson Integrated metagenomics/metaproteomics reveals human host-microbiota signatures of Crohn's disease.
description	Crohn's disease (CD) is an inflammatory bowel disease of complex etiology, although dysbiosis of the gut microbiota has been implicated in chronic immune-mediated inflammation associated with CD. Here we combined shotgun metagenomic and metaproteomic approaches to identify potential functional signatures of CD in stool samples from six twin pairs that were either healthy, or that had CD in the ileum (ICD) or colon (CCD). Integration of these omics approaches revealed several genes, proteins, and pathways that primarily differentiated ICD from healthy subjects, including depletion of many proteins in ICD. In addition, the ICD phenotype was associated with alterations in bacterial carbohydrate metabolism, bacterial-host interactions, as well as human host-secreted enzymes. This eco-systems biology approach underscores the link between the gut microbiota and functional alterations in the pathophysiology of Crohn's disease and aids in identification of novel diagnostic targets and disease specific biomarkers.
format	article
author	Alison R Erickson Brandi L Cantarel Regina Lamendella Youssef Darzi Emmanuel F Mongodin Chongle Pan Manesh Shah Jonas Halfvarson Curt Tysk Bernard Henrissat Jeroen Raes Nathan C Verberkmoes Claire M Fraser Robert L Hettich Janet K Jansson
author_facet	Alison R Erickson Brandi L Cantarel Regina Lamendella Youssef Darzi Emmanuel F Mongodin Chongle Pan Manesh Shah Jonas Halfvarson Curt Tysk Bernard Henrissat Jeroen Raes Nathan C Verberkmoes Claire M Fraser Robert L Hettich Janet K Jansson
author_sort	Alison R Erickson
title	Integrated metagenomics/metaproteomics reveals human host-microbiota signatures of Crohn's disease.
title_short	Integrated metagenomics/metaproteomics reveals human host-microbiota signatures of Crohn's disease.
title_full	Integrated metagenomics/metaproteomics reveals human host-microbiota signatures of Crohn's disease.
title_fullStr	Integrated metagenomics/metaproteomics reveals human host-microbiota signatures of Crohn's disease.
title_full_unstemmed	Integrated metagenomics/metaproteomics reveals human host-microbiota signatures of Crohn's disease.
title_sort	integrated metagenomics/metaproteomics reveals human host-microbiota signatures of crohn's disease.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/0ba838e8eed447ac81829dfa7897d160
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Integrated metagenomics/metaproteomics reveals human host-microbiota signatures of Crohn's disease.

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