Microbiota dynamics in patients treated with fecal microbiota transplantation for recurrent Clostridium difficile infection.

Clostridium difficile causes antibiotic-associated diarrhea and pseudomembraneous colitis and is responsible for a large and increasing fraction of hospital-acquired infections. Fecal microbiota transplantation (FMT) is an alternate treatment option for recurrent C. difficile infection (RCDI) refrac...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yang Song, Shashank Garg, Mohit Girotra, Cynthia Maddox, Erik C von Rosenvinge, Anand Dutta, Sudhir Dutta, W Florian Fricke
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0baf93419ab64cff96d92a8e6e88dcd8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0baf93419ab64cff96d92a8e6e88dcd8
record_format	dspace
spelling	oai:doaj.org-article:0baf93419ab64cff96d92a8e6e88dcd82021-11-18T08:44:34ZMicrobiota dynamics in patients treated with fecal microbiota transplantation for recurrent Clostridium difficile infection.1932-620310.1371/journal.pone.0081330https://doaj.org/article/0baf93419ab64cff96d92a8e6e88dcd82013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24303043/?tool=EBIhttps://doaj.org/toc/1932-6203Clostridium difficile causes antibiotic-associated diarrhea and pseudomembraneous colitis and is responsible for a large and increasing fraction of hospital-acquired infections. Fecal microbiota transplantation (FMT) is an alternate treatment option for recurrent C. difficile infection (RCDI) refractory to antibiotic therapy. It has recently been discussed favorably in the clinical and scientific communities and is receiving increasing public attention. However, short- and long-term health consequences of FMT remain a concern, as the effects of the transplanted microbiota on the patient remain unknown. To shed light on microbial events associated with RCDI and treatment by FMT, we performed fecal microbiota analysis by 16S rRNA gene amplicon pyrosequencing of 14 pairs of healthy donors and RCDI patients treated successfully by FMT. Post-FMT patient and healthy donor samples collected up to one year after FMT were studied longitudinally, including one post-FMT patient with antibiotic-associated relapse three months after FMT. This analysis allowed us not only to confirm prior reports that RCDI is associated with reduced diversity and compositional changes in the fecal microbiota, but also to characterize previously undocumented post-FMT microbiota dynamics. Members of the Streptococcaceae, Enterococcaceae, or Enterobacteriaceae were significantly increased and putative butyrate producers, such as Lachnospiraceae and Ruminococcaceae were significantly reduced in samples from RCDI patients before FMT as compared to post-FMT patient and healthy donor samples. RCDI patient samples showed more case-specific variations than post-FMT patient and healthy donor samples. However, none of the bacterial groups were invariably associated with RCDI or successful treatment by FMT. Overall microbiota compositions in post-FMT patients, specifically abundances of the above-mentioned Firmicutes, continued to change for at least 16 weeks after FMT, suggesting that full microbiota recovery from RCDI may take much longer than expected based on the disappearance of diarrheal symptoms immediately after FMT.Yang SongShashank GargMohit GirotraCynthia MaddoxErik C von RosenvingeAnand DuttaSudhir DuttaW Florian FrickePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e81330 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yang Song Shashank Garg Mohit Girotra Cynthia Maddox Erik C von Rosenvinge Anand Dutta Sudhir Dutta W Florian Fricke Microbiota dynamics in patients treated with fecal microbiota transplantation for recurrent Clostridium difficile infection.
description	Clostridium difficile causes antibiotic-associated diarrhea and pseudomembraneous colitis and is responsible for a large and increasing fraction of hospital-acquired infections. Fecal microbiota transplantation (FMT) is an alternate treatment option for recurrent C. difficile infection (RCDI) refractory to antibiotic therapy. It has recently been discussed favorably in the clinical and scientific communities and is receiving increasing public attention. However, short- and long-term health consequences of FMT remain a concern, as the effects of the transplanted microbiota on the patient remain unknown. To shed light on microbial events associated with RCDI and treatment by FMT, we performed fecal microbiota analysis by 16S rRNA gene amplicon pyrosequencing of 14 pairs of healthy donors and RCDI patients treated successfully by FMT. Post-FMT patient and healthy donor samples collected up to one year after FMT were studied longitudinally, including one post-FMT patient with antibiotic-associated relapse three months after FMT. This analysis allowed us not only to confirm prior reports that RCDI is associated with reduced diversity and compositional changes in the fecal microbiota, but also to characterize previously undocumented post-FMT microbiota dynamics. Members of the Streptococcaceae, Enterococcaceae, or Enterobacteriaceae were significantly increased and putative butyrate producers, such as Lachnospiraceae and Ruminococcaceae were significantly reduced in samples from RCDI patients before FMT as compared to post-FMT patient and healthy donor samples. RCDI patient samples showed more case-specific variations than post-FMT patient and healthy donor samples. However, none of the bacterial groups were invariably associated with RCDI or successful treatment by FMT. Overall microbiota compositions in post-FMT patients, specifically abundances of the above-mentioned Firmicutes, continued to change for at least 16 weeks after FMT, suggesting that full microbiota recovery from RCDI may take much longer than expected based on the disappearance of diarrheal symptoms immediately after FMT.
format	article
author	Yang Song Shashank Garg Mohit Girotra Cynthia Maddox Erik C von Rosenvinge Anand Dutta Sudhir Dutta W Florian Fricke
author_facet	Yang Song Shashank Garg Mohit Girotra Cynthia Maddox Erik C von Rosenvinge Anand Dutta Sudhir Dutta W Florian Fricke
author_sort	Yang Song
title	Microbiota dynamics in patients treated with fecal microbiota transplantation for recurrent Clostridium difficile infection.
title_short	Microbiota dynamics in patients treated with fecal microbiota transplantation for recurrent Clostridium difficile infection.
title_full	Microbiota dynamics in patients treated with fecal microbiota transplantation for recurrent Clostridium difficile infection.
title_fullStr	Microbiota dynamics in patients treated with fecal microbiota transplantation for recurrent Clostridium difficile infection.
title_full_unstemmed	Microbiota dynamics in patients treated with fecal microbiota transplantation for recurrent Clostridium difficile infection.
title_sort	microbiota dynamics in patients treated with fecal microbiota transplantation for recurrent clostridium difficile infection.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/0baf93419ab64cff96d92a8e6e88dcd8
work_keys_str_mv	AT yangsong microbiotadynamicsinpatientstreatedwithfecalmicrobiotatransplantationforrecurrentclostridiumdifficileinfection AT shashankgarg microbiotadynamicsinpatientstreatedwithfecalmicrobiotatransplantationforrecurrentclostridiumdifficileinfection AT mohitgirotra microbiotadynamicsinpatientstreatedwithfecalmicrobiotatransplantationforrecurrentclostridiumdifficileinfection AT cynthiamaddox microbiotadynamicsinpatientstreatedwithfecalmicrobiotatransplantationforrecurrentclostridiumdifficileinfection AT erikcvonrosenvinge microbiotadynamicsinpatientstreatedwithfecalmicrobiotatransplantationforrecurrentclostridiumdifficileinfection AT ananddutta microbiotadynamicsinpatientstreatedwithfecalmicrobiotatransplantationforrecurrentclostridiumdifficileinfection AT sudhirdutta microbiotadynamicsinpatientstreatedwithfecalmicrobiotatransplantationforrecurrentclostridiumdifficileinfection AT wflorianfricke microbiotadynamicsinpatientstreatedwithfecalmicrobiotatransplantationforrecurrentclostridiumdifficileinfection
_version_	1718421364573995008

Microbiota dynamics in patients treated with fecal microbiota transplantation for recurrent Clostridium difficile infection.

Ejemplares similares