Study of Histopathological Lesions in CA1 of the Hippocampus after Injection of Beta-Amyloid in a Rat Model of Alzheimer’s Disease

Study of Histopathological Lesions in CA1 of the Hippocampus after Injection of Beta-Amyloid in a Rat Model of Alzheimer’s Disease

BACKGROUND AND OBJECTIVE: Alzheimer’s disease (AD) is a neurodegenerative disorder and the most common form of degenerative dementia with progressive loss of cognitive abilities and memory loss. AD is an irreversible, progressive chronic disease that it is the cause of behavior changes and deteriora...

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Autores principales: SM Hoseini, M Nobakht, P Mortazavi, B Esmailzade, N Rahbar-Rooshandel, SH Omidzahir
Formato: article
Lenguaje:EN
FA
Publicado: Babol University of Medical Sciences 2012
Materias:
alzheimers disease (ad)
hippocampus
beta-amyloid (abeta)
memory
neuropathological changes
Medicine
R
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/0bb7aa72dead4cac9f4ae4081aefe982
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spelling oai:doaj.org-article:0bb7aa72dead4cac9f4ae4081aefe9822021-11-10T08:53:19ZStudy of Histopathological Lesions in CA1 of the Hippocampus after Injection of Beta-Amyloid in a Rat Model of Alzheimer’s Disease1561-41072251-7170https://doaj.org/article/0bb7aa72dead4cac9f4ae4081aefe9822012-07-01T00:00:00Zhttp://jbums.org/article-1-4144-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Alzheimer’s disease (AD) is a neurodegenerative disorder and the most common form of degenerative dementia with progressive loss of cognitive abilities and memory loss. AD is an irreversible, progressive chronic disease that it is the cause of behavior changes and deterioration of thinking ability. Since exact mechanism of neuro-toxicity by beta amyloid has not been identified yet, in this study, the histopathological lesions in CA1 of hippocampus after injection of beta-amyloid in a rat model of AD was studied.METHODS: In this experimental study, 30 adult male Albino Wistar rats weighing (250-300 g) were used for behavioral and histopathological studies. The rats were randomly assigned to three groups control, sham and BETA-amyloid (ABETA) injection. The lesion was induced by injection of 4µLof ABETA(1-40) into the hippocampal fissure. For behavioral analysis Y-maze and shuttle box were used respectively at the 14 and 16 days post-lesion. For histological studies, Nissl and Bielschowsky staining were done.FINDINGS: The mean scores of alternation behavior for sham, control, and AD group were 80.56%, 86.7%, and 46.2%, respectively. AD group showed a significant reduction in alternation behavior as compared to control and sham group (p<0.0001). The number of neurons per square millimeter in the Alzheimer group, sham and control, respectively, 2.92, 6.35 and 6.25, and reduced neuronal density in Alzheimers disease compared with control and sham groups were significant (p<0.0001).CONCLUSION: In the present study,ABETA(1-40) injection into hippocampus could decrease the behavioral indexes and the number of CA1 neurons in hippocampus.SM Hoseini,M Nobakht,P MortazaviB Esmailzade,N Rahbar-Rooshandel,SH OmidzahirBabol University of Medical Sciencesarticlealzheimers disease (ad)hippocampusbeta-amyloid (abeta)memoryneuropathological changesMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 14, Iss 4, Pp 90-96 (2012)
institution DOAJ
collection DOAJ
language EN
FA
topic alzheimers disease (ad)
hippocampus
beta-amyloid (abeta)
memory
neuropathological changes
Medicine
R
Medicine (General)
R5-920
spellingShingle alzheimers disease (ad)
hippocampus
beta-amyloid (abeta)
memory
neuropathological changes
Medicine
R
Medicine (General)
R5-920
SM Hoseini,
M Nobakht,
P Mortazavi
B Esmailzade,
N Rahbar-Rooshandel,
SH Omidzahir
Study of Histopathological Lesions in CA1 of the Hippocampus after Injection of Beta-Amyloid in a Rat Model of Alzheimer’s Disease
description BACKGROUND AND OBJECTIVE: Alzheimer’s disease (AD) is a neurodegenerative disorder and the most common form of degenerative dementia with progressive loss of cognitive abilities and memory loss. AD is an irreversible, progressive chronic disease that it is the cause of behavior changes and deterioration of thinking ability. Since exact mechanism of neuro-toxicity by beta amyloid has not been identified yet, in this study, the histopathological lesions in CA1 of hippocampus after injection of beta-amyloid in a rat model of AD was studied.METHODS: In this experimental study, 30 adult male Albino Wistar rats weighing (250-300 g) were used for behavioral and histopathological studies. The rats were randomly assigned to three groups control, sham and BETA-amyloid (ABETA) injection. The lesion was induced by injection of 4µLof ABETA(1-40) into the hippocampal fissure. For behavioral analysis Y-maze and shuttle box were used respectively at the 14 and 16 days post-lesion. For histological studies, Nissl and Bielschowsky staining were done.FINDINGS: The mean scores of alternation behavior for sham, control, and AD group were 80.56%, 86.7%, and 46.2%, respectively. AD group showed a significant reduction in alternation behavior as compared to control and sham group (p<0.0001). The number of neurons per square millimeter in the Alzheimer group, sham and control, respectively, 2.92, 6.35 and 6.25, and reduced neuronal density in Alzheimers disease compared with control and sham groups were significant (p<0.0001).CONCLUSION: In the present study,ABETA(1-40) injection into hippocampus could decrease the behavioral indexes and the number of CA1 neurons in hippocampus.
format article
author SM Hoseini,
M Nobakht,
P Mortazavi
B Esmailzade,
N Rahbar-Rooshandel,
SH Omidzahir
author_facet SM Hoseini,
M Nobakht,
P Mortazavi
B Esmailzade,
N Rahbar-Rooshandel,
SH Omidzahir
author_sort SM Hoseini,
title Study of Histopathological Lesions in CA1 of the Hippocampus after Injection of Beta-Amyloid in a Rat Model of Alzheimer’s Disease
title_short Study of Histopathological Lesions in CA1 of the Hippocampus after Injection of Beta-Amyloid in a Rat Model of Alzheimer’s Disease
title_full Study of Histopathological Lesions in CA1 of the Hippocampus after Injection of Beta-Amyloid in a Rat Model of Alzheimer’s Disease
title_fullStr Study of Histopathological Lesions in CA1 of the Hippocampus after Injection of Beta-Amyloid in a Rat Model of Alzheimer’s Disease
title_full_unstemmed Study of Histopathological Lesions in CA1 of the Hippocampus after Injection of Beta-Amyloid in a Rat Model of Alzheimer’s Disease
title_sort study of histopathological lesions in ca1 of the hippocampus after injection of beta-amyloid in a rat model of alzheimer’s disease
publisher Babol University of Medical Sciences
publishDate 2012
url https://doaj.org/article/0bb7aa72dead4cac9f4ae4081aefe982
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