Zeolite-based nanocomposite as a smart pH-sensitive nanovehicle for release of xylanase as poultry feed supplement

Abstract Xylanase improves poultry nutrition by degrading xylan in the cell walls of feed grains and release the entrapped nutrients. However, the application of xylanase as a feed supplement is restricted to its low stability in the environment and gastrointestinal (GI) tract of poultry. To overcom...

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Autores principales:	Fariba Dashtestani, Leila Ma’mani, Farzaneh Jokar, Morteza Maleki, Mohammad Eskandari Fard, Ghasem Hosseini Salekdeh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0bb831c7e856400b97a6a01bae310f87
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spelling	oai:doaj.org-article:0bb831c7e856400b97a6a01bae310f872021-11-08T10:53:51ZZeolite-based nanocomposite as a smart pH-sensitive nanovehicle for release of xylanase as poultry feed supplement10.1038/s41598-021-00688-72045-2322https://doaj.org/article/0bb831c7e856400b97a6a01bae310f872021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-00688-7https://doaj.org/toc/2045-2322Abstract Xylanase improves poultry nutrition by degrading xylan in the cell walls of feed grains and release the entrapped nutrients. However, the application of xylanase as a feed supplement is restricted to its low stability in the environment and gastrointestinal (GI) tract of poultry. To overcome these obstacles, Zeozyme NPs as a smart pH-responsive nanosystem was designed based on xylanase immobilization on zeolitic nanoporous as the major cornerstone that was modified with L-lysine. The immobilized xylanase was followed by encapsulating with a cross-linked CMC-based polymer. Zeozyme NPs was structurally characterized using TEM, SEM, AFM, DLS, TGA and nitrogen adsorption/desorption isotherms at liquid nitrogen temperature. The stability of Zeozyme NPs was evaluated at different temperatures, pH, and in the presence of proteases. Additionally, the release pattern of xylanase was investigated at a digestion model mimicking the GI tract. Xylanase was released selectively at the duodenum and ileum (pH 6–7.1) and remarkably preserved at pH ≤ 6 including proventriculus, gizzard, and crop (pH 1.6–5). The results confirmed that the zeolite equipped with the CMC matrix could enhance the xylanase thermal and pH stability and preserve its activity in the presence of proteases. Moreover, Zeozyme NPs exhibited a smart pH-dependent release of xylanase in an in vitro simulated GI tract.Fariba DashtestaniLeila Ma’maniFarzaneh JokarMorteza MalekiMohammad Eskandari FardGhasem Hosseini SalekdehNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fariba Dashtestani Leila Ma’mani Farzaneh Jokar Morteza Maleki Mohammad Eskandari Fard Ghasem Hosseini Salekdeh Zeolite-based nanocomposite as a smart pH-sensitive nanovehicle for release of xylanase as poultry feed supplement
description	Abstract Xylanase improves poultry nutrition by degrading xylan in the cell walls of feed grains and release the entrapped nutrients. However, the application of xylanase as a feed supplement is restricted to its low stability in the environment and gastrointestinal (GI) tract of poultry. To overcome these obstacles, Zeozyme NPs as a smart pH-responsive nanosystem was designed based on xylanase immobilization on zeolitic nanoporous as the major cornerstone that was modified with L-lysine. The immobilized xylanase was followed by encapsulating with a cross-linked CMC-based polymer. Zeozyme NPs was structurally characterized using TEM, SEM, AFM, DLS, TGA and nitrogen adsorption/desorption isotherms at liquid nitrogen temperature. The stability of Zeozyme NPs was evaluated at different temperatures, pH, and in the presence of proteases. Additionally, the release pattern of xylanase was investigated at a digestion model mimicking the GI tract. Xylanase was released selectively at the duodenum and ileum (pH 6–7.1) and remarkably preserved at pH ≤ 6 including proventriculus, gizzard, and crop (pH 1.6–5). The results confirmed that the zeolite equipped with the CMC matrix could enhance the xylanase thermal and pH stability and preserve its activity in the presence of proteases. Moreover, Zeozyme NPs exhibited a smart pH-dependent release of xylanase in an in vitro simulated GI tract.
format	article
author	Fariba Dashtestani Leila Ma’mani Farzaneh Jokar Morteza Maleki Mohammad Eskandari Fard Ghasem Hosseini Salekdeh
author_facet	Fariba Dashtestani Leila Ma’mani Farzaneh Jokar Morteza Maleki Mohammad Eskandari Fard Ghasem Hosseini Salekdeh
author_sort	Fariba Dashtestani
title	Zeolite-based nanocomposite as a smart pH-sensitive nanovehicle for release of xylanase as poultry feed supplement
title_short	Zeolite-based nanocomposite as a smart pH-sensitive nanovehicle for release of xylanase as poultry feed supplement
title_full	Zeolite-based nanocomposite as a smart pH-sensitive nanovehicle for release of xylanase as poultry feed supplement
title_fullStr	Zeolite-based nanocomposite as a smart pH-sensitive nanovehicle for release of xylanase as poultry feed supplement
title_full_unstemmed	Zeolite-based nanocomposite as a smart pH-sensitive nanovehicle for release of xylanase as poultry feed supplement
title_sort	zeolite-based nanocomposite as a smart ph-sensitive nanovehicle for release of xylanase as poultry feed supplement
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0bb831c7e856400b97a6a01bae310f87
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Zeolite-based nanocomposite as a smart pH-sensitive nanovehicle for release of xylanase as poultry feed supplement

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