Kinetics of the invasion and egress processes of Babesia divergens, observed by time-lapse video microscopy

Abstract Based on confocal fluorescence and bright field video microscopy, we present detailed observations on the processes of invasion and egress of erythrocytes by the apicomplexan parasite Babesia divergens. Time-lapse images reveal numerous unexpected findings associated with the dynamics of B....

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Autores principales:	Elena Sevilla, Luis Miguel González, Daniel Luque, Jeremy Gray, Estrella Montero
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Egress Process Time-lapse Videomicroscopy Divergent Parasite Sister Cells Free Merozoites Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0bbbd91abc164b5c8ae021d567bc8ea6
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spelling	oai:doaj.org-article:0bbbd91abc164b5c8ae021d567bc8ea62021-12-02T15:08:16ZKinetics of the invasion and egress processes of Babesia divergens, observed by time-lapse video microscopy10.1038/s41598-018-32349-72045-2322https://doaj.org/article/0bbbd91abc164b5c8ae021d567bc8ea62018-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-32349-7https://doaj.org/toc/2045-2322Abstract Based on confocal fluorescence and bright field video microscopy, we present detailed observations on the processes of invasion and egress of erythrocytes by the apicomplexan parasite Babesia divergens. Time-lapse images reveal numerous unexpected findings associated with the dynamics of B. divergens and its ability to manipulate the erythrocyte during both processes in its asexual cycle under in vitro conditions. Despite the speed at which these processes occur and the small size of the parasite, we capture infective merozoites moving vigorously and causing striking deformations in the erythrocyte’s plasma membrane during an active invasion. We also observed intraerythrocytic dynamic stages as paired pyriforms, double paired pyriforms, tetrads, unattached pyriform sister cells and multiple parasite stages resulting in the release of large numbers of merozoites over a short period. Of considerable interest is that time-lapse images reveal a novel mechanism of egress used by B. divergens to exit the human erythrocyte. The release occurs when B. divergens parasites establish contacts with the plasma membrane of the erythrocyte from within, before exiting the cell. Visualization and analysis of the images enabled us to obtain useful information and broaden our knowledge of complex and crucial events involved with parasitisation of human erythrocytes by B. divergens.Elena SevillaLuis Miguel GonzálezDaniel LuqueJeremy GrayEstrella MonteroNature PortfolioarticleEgress ProcessTime-lapse VideomicroscopyDivergent ParasiteSister CellsFree MerozoitesMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Egress Process Time-lapse Videomicroscopy Divergent Parasite Sister Cells Free Merozoites Medicine R Science Q
spellingShingle	Egress Process Time-lapse Videomicroscopy Divergent Parasite Sister Cells Free Merozoites Medicine R Science Q Elena Sevilla Luis Miguel González Daniel Luque Jeremy Gray Estrella Montero Kinetics of the invasion and egress processes of Babesia divergens, observed by time-lapse video microscopy
description	Abstract Based on confocal fluorescence and bright field video microscopy, we present detailed observations on the processes of invasion and egress of erythrocytes by the apicomplexan parasite Babesia divergens. Time-lapse images reveal numerous unexpected findings associated with the dynamics of B. divergens and its ability to manipulate the erythrocyte during both processes in its asexual cycle under in vitro conditions. Despite the speed at which these processes occur and the small size of the parasite, we capture infective merozoites moving vigorously and causing striking deformations in the erythrocyte’s plasma membrane during an active invasion. We also observed intraerythrocytic dynamic stages as paired pyriforms, double paired pyriforms, tetrads, unattached pyriform sister cells and multiple parasite stages resulting in the release of large numbers of merozoites over a short period. Of considerable interest is that time-lapse images reveal a novel mechanism of egress used by B. divergens to exit the human erythrocyte. The release occurs when B. divergens parasites establish contacts with the plasma membrane of the erythrocyte from within, before exiting the cell. Visualization and analysis of the images enabled us to obtain useful information and broaden our knowledge of complex and crucial events involved with parasitisation of human erythrocytes by B. divergens.
format	article
author	Elena Sevilla Luis Miguel González Daniel Luque Jeremy Gray Estrella Montero
author_facet	Elena Sevilla Luis Miguel González Daniel Luque Jeremy Gray Estrella Montero
author_sort	Elena Sevilla
title	Kinetics of the invasion and egress processes of Babesia divergens, observed by time-lapse video microscopy
title_short	Kinetics of the invasion and egress processes of Babesia divergens, observed by time-lapse video microscopy
title_full	Kinetics of the invasion and egress processes of Babesia divergens, observed by time-lapse video microscopy
title_fullStr	Kinetics of the invasion and egress processes of Babesia divergens, observed by time-lapse video microscopy
title_full_unstemmed	Kinetics of the invasion and egress processes of Babesia divergens, observed by time-lapse video microscopy
title_sort	kinetics of the invasion and egress processes of babesia divergens, observed by time-lapse video microscopy
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/0bbbd91abc164b5c8ae021d567bc8ea6
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Kinetics of the invasion and egress processes of Babesia divergens, observed by time-lapse video microscopy

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