In vivo micro-CT assessment of airway remodeling in a flexible OVA-sensitized murine model of asthma.

Airway remodeling is a major pathological feature of asthma. Up to now, its quantification still requires invasive methods. In this study, we aimed at determining whether in vivo micro-computed tomography (micro-CT) is able to demonstrate allergen-induced airway remodeling in a flexible mouse model...

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Autores principales:	Mathieu Lederlin, Annaïg Ozier, Gaël Dournes, Olga Ousova, Pierre-Olivier Girodet, Hugues Begueret, Roger Marthan, Michel Montaudon, François Laurent, Patrick Berger
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0bbc92563fe14dc983fee07b42a58aec
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spelling	oai:doaj.org-article:0bbc92563fe14dc983fee07b42a58aec2021-11-18T08:10:36ZIn vivo micro-CT assessment of airway remodeling in a flexible OVA-sensitized murine model of asthma.1932-620310.1371/journal.pone.0048493https://doaj.org/article/0bbc92563fe14dc983fee07b42a58aec2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23119036/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Airway remodeling is a major pathological feature of asthma. Up to now, its quantification still requires invasive methods. In this study, we aimed at determining whether in vivo micro-computed tomography (micro-CT) is able to demonstrate allergen-induced airway remodeling in a flexible mouse model of asthma. Sixty Balb/c mice were challenged intranasally with ovalbumin or saline at 3 different endpoints (Days 35, 75, and 110). All mice underwent plethysmography at baseline and just prior to respiratory-gated micro-CT. Mice were then sacrificed to assess bronchoalveolar lavage and lung histology. From micro-CT images (voxel size = 46×46×46 µm), the numerical values of total lung attenuation, peribronchial attenuation (PBA), and PBA normalized by total lung attenuation were extracted. Each parameter was compared between OVA and control mice and correlation coefficients were calculated between micro-CT and histological data. As compared to control animals, ovalbumin-sensitized mice exhibited inflammation alone (Day 35), remodeling alone (Day 110) or both inflammation and remodeling (Day 75). Normalized PBA was significantly greater in mice exhibiting bronchial remodeling either alone or in combination with inflammation. Normalized PBA correlated with various remodeling markers such as bronchial smooth muscle size or peribronchial fibrosis. These findings suggest that micro-CT may help monitor remodeling non-invasively in asthmatic mice when testing new drugs targeting airway remodeling in pre-clinical studies.Mathieu LederlinAnnaïg OzierGaël DournesOlga OusovaPierre-Olivier GirodetHugues BegueretRoger MarthanMichel MontaudonFrançois LaurentPatrick BergerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 10, p e48493 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mathieu Lederlin Annaïg Ozier Gaël Dournes Olga Ousova Pierre-Olivier Girodet Hugues Begueret Roger Marthan Michel Montaudon François Laurent Patrick Berger In vivo micro-CT assessment of airway remodeling in a flexible OVA-sensitized murine model of asthma.
description	Airway remodeling is a major pathological feature of asthma. Up to now, its quantification still requires invasive methods. In this study, we aimed at determining whether in vivo micro-computed tomography (micro-CT) is able to demonstrate allergen-induced airway remodeling in a flexible mouse model of asthma. Sixty Balb/c mice were challenged intranasally with ovalbumin or saline at 3 different endpoints (Days 35, 75, and 110). All mice underwent plethysmography at baseline and just prior to respiratory-gated micro-CT. Mice were then sacrificed to assess bronchoalveolar lavage and lung histology. From micro-CT images (voxel size = 46×46×46 µm), the numerical values of total lung attenuation, peribronchial attenuation (PBA), and PBA normalized by total lung attenuation were extracted. Each parameter was compared between OVA and control mice and correlation coefficients were calculated between micro-CT and histological data. As compared to control animals, ovalbumin-sensitized mice exhibited inflammation alone (Day 35), remodeling alone (Day 110) or both inflammation and remodeling (Day 75). Normalized PBA was significantly greater in mice exhibiting bronchial remodeling either alone or in combination with inflammation. Normalized PBA correlated with various remodeling markers such as bronchial smooth muscle size or peribronchial fibrosis. These findings suggest that micro-CT may help monitor remodeling non-invasively in asthmatic mice when testing new drugs targeting airway remodeling in pre-clinical studies.
format	article
author	Mathieu Lederlin Annaïg Ozier Gaël Dournes Olga Ousova Pierre-Olivier Girodet Hugues Begueret Roger Marthan Michel Montaudon François Laurent Patrick Berger
author_facet	Mathieu Lederlin Annaïg Ozier Gaël Dournes Olga Ousova Pierre-Olivier Girodet Hugues Begueret Roger Marthan Michel Montaudon François Laurent Patrick Berger
author_sort	Mathieu Lederlin
title	In vivo micro-CT assessment of airway remodeling in a flexible OVA-sensitized murine model of asthma.
title_short	In vivo micro-CT assessment of airway remodeling in a flexible OVA-sensitized murine model of asthma.
title_full	In vivo micro-CT assessment of airway remodeling in a flexible OVA-sensitized murine model of asthma.
title_fullStr	In vivo micro-CT assessment of airway remodeling in a flexible OVA-sensitized murine model of asthma.
title_full_unstemmed	In vivo micro-CT assessment of airway remodeling in a flexible OVA-sensitized murine model of asthma.
title_sort	in vivo micro-ct assessment of airway remodeling in a flexible ova-sensitized murine model of asthma.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/0bbc92563fe14dc983fee07b42a58aec
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In vivo micro-CT assessment of airway remodeling in a flexible OVA-sensitized murine model of asthma.

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