<i>Peucedanum japonicum</i> Thunberg and Its Active Components Mitigate Oxidative Stress, Inflammation and Apoptosis after Urban Particulate Matter-Induced Ocular Surface Damage

We previously demonstrated that urban particulate matter (UPM) exposure decreases the migration activity and survival of human corneal epithelial cells (HCECs). Herein, we investigated the potential to improve the corneal wound-healing ability of <i>Peucedanum japonicum</i> Thunb. leaf e...

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Autores principales:	Wan Seok Kang, Hakjoon Choi, Ki Hoon Lee, Eun Kim, Kyeong Jo Kim, Jin Seok Kim, Chang-Su Na, Sunoh Kim
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	urban particulate matter (UPM) <i>Peucedanum japonicum</i> Thunberg corneal epithelial cells wound healing antioxidant inflammation Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/0bc0e822718e4e1aa830f04b3c6453d8
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spelling	oai:doaj.org-article:0bc0e822718e4e1aa830f04b3c6453d82021-11-25T16:27:06Z<i>Peucedanum japonicum</i> Thunberg and Its Active Components Mitigate Oxidative Stress, Inflammation and Apoptosis after Urban Particulate Matter-Induced Ocular Surface Damage10.3390/antiox101117172076-3921https://doaj.org/article/0bc0e822718e4e1aa830f04b3c6453d82021-10-01T00:00:00Zhttps://www.mdpi.com/2076-3921/10/11/1717https://doaj.org/toc/2076-3921We previously demonstrated that urban particulate matter (UPM) exposure decreases the migration activity and survival of human corneal epithelial cells (HCECs). Herein, we investigated the potential to improve the corneal wound-healing ability of <i>Peucedanum japonicum</i> Thunb. leaf extract (PJE) and its active components on UPM-induced ocular surface damage in vitro and in vivo. PJE effectively assisted wound healing without altering HCEC survival and enhanced catalase (CAT), heme oxygenase 1 (HO1) and glutathione peroxidase 1 (GPX1) antioxidant gene expression. A corneal wound was uniformly induced on the right eye in all experimental animals and divided into eight groups such as two control groups (wounded right eye group—NR and non-wounded left eye group—NL), UPM treated group and PJEs (25, 50, 100, 200, 400 mg/kg) treated groups. Corneal abrasion model rats exposed to UPM showed delayed wound healing compared to unexposed rats, but wound healing was dose-dependently enhanced by PJE oral administration. Seventy-two hours after wound generation, inflammatory cells, apoptotic cells and interleukin-6 (IL-6) expression were increased substantially after UPM exposure, but PJE treatment significantly reduced the wound to an almost normal level while enhancing re-epithelialization without changing corneal thickness. Next, we tried to identify the key molecules for enhancing wound healing through fractionation. The major compounds in the fraction, confirmed by high-performance liquid chromatography (HPLC), were chlorogenic acid (CA), neochlorogenic acid (NCA) and cryptochlorogenic acid (CCA). Each type of CA isomers showed slightly different half maximal effective (EC50) and maximal effective (ECmax) concentrations, and their mixtures synergistically enhanced HCEC migration. Thus, corneal abrasion wound recovery after UPM exposure improved after PJE treatment, and the active PJE components were identified, providing an important basis to develop therapeutics for ocular surface damage using PJE.Wan Seok KangHakjoon ChoiKi Hoon LeeEun KimKyeong Jo KimJin Seok KimChang-Su NaSunoh KimMDPI AGarticleurban particulate matter (UPM)<i>Peucedanum japonicum</i> Thunbergcorneal epithelial cellswound healingantioxidantinflammationTherapeutics. PharmacologyRM1-950ENAntioxidants, Vol 10, Iss 1717, p 1717 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	urban particulate matter (UPM) <i>Peucedanum japonicum</i> Thunberg corneal epithelial cells wound healing antioxidant inflammation Therapeutics. Pharmacology RM1-950
spellingShingle	urban particulate matter (UPM) <i>Peucedanum japonicum</i> Thunberg corneal epithelial cells wound healing antioxidant inflammation Therapeutics. Pharmacology RM1-950 Wan Seok Kang Hakjoon Choi Ki Hoon Lee Eun Kim Kyeong Jo Kim Jin Seok Kim Chang-Su Na Sunoh Kim <i>Peucedanum japonicum</i> Thunberg and Its Active Components Mitigate Oxidative Stress, Inflammation and Apoptosis after Urban Particulate Matter-Induced Ocular Surface Damage
description	We previously demonstrated that urban particulate matter (UPM) exposure decreases the migration activity and survival of human corneal epithelial cells (HCECs). Herein, we investigated the potential to improve the corneal wound-healing ability of <i>Peucedanum japonicum</i> Thunb. leaf extract (PJE) and its active components on UPM-induced ocular surface damage in vitro and in vivo. PJE effectively assisted wound healing without altering HCEC survival and enhanced catalase (CAT), heme oxygenase 1 (HO1) and glutathione peroxidase 1 (GPX1) antioxidant gene expression. A corneal wound was uniformly induced on the right eye in all experimental animals and divided into eight groups such as two control groups (wounded right eye group—NR and non-wounded left eye group—NL), UPM treated group and PJEs (25, 50, 100, 200, 400 mg/kg) treated groups. Corneal abrasion model rats exposed to UPM showed delayed wound healing compared to unexposed rats, but wound healing was dose-dependently enhanced by PJE oral administration. Seventy-two hours after wound generation, inflammatory cells, apoptotic cells and interleukin-6 (IL-6) expression were increased substantially after UPM exposure, but PJE treatment significantly reduced the wound to an almost normal level while enhancing re-epithelialization without changing corneal thickness. Next, we tried to identify the key molecules for enhancing wound healing through fractionation. The major compounds in the fraction, confirmed by high-performance liquid chromatography (HPLC), were chlorogenic acid (CA), neochlorogenic acid (NCA) and cryptochlorogenic acid (CCA). Each type of CA isomers showed slightly different half maximal effective (EC50) and maximal effective (ECmax) concentrations, and their mixtures synergistically enhanced HCEC migration. Thus, corneal abrasion wound recovery after UPM exposure improved after PJE treatment, and the active PJE components were identified, providing an important basis to develop therapeutics for ocular surface damage using PJE.
format	article
author	Wan Seok Kang Hakjoon Choi Ki Hoon Lee Eun Kim Kyeong Jo Kim Jin Seok Kim Chang-Su Na Sunoh Kim
author_facet	Wan Seok Kang Hakjoon Choi Ki Hoon Lee Eun Kim Kyeong Jo Kim Jin Seok Kim Chang-Su Na Sunoh Kim
author_sort	Wan Seok Kang
title	<i>Peucedanum japonicum</i> Thunberg and Its Active Components Mitigate Oxidative Stress, Inflammation and Apoptosis after Urban Particulate Matter-Induced Ocular Surface Damage
title_short	<i>Peucedanum japonicum</i> Thunberg and Its Active Components Mitigate Oxidative Stress, Inflammation and Apoptosis after Urban Particulate Matter-Induced Ocular Surface Damage
title_full	<i>Peucedanum japonicum</i> Thunberg and Its Active Components Mitigate Oxidative Stress, Inflammation and Apoptosis after Urban Particulate Matter-Induced Ocular Surface Damage
title_fullStr	<i>Peucedanum japonicum</i> Thunberg and Its Active Components Mitigate Oxidative Stress, Inflammation and Apoptosis after Urban Particulate Matter-Induced Ocular Surface Damage
title_full_unstemmed	<i>Peucedanum japonicum</i> Thunberg and Its Active Components Mitigate Oxidative Stress, Inflammation and Apoptosis after Urban Particulate Matter-Induced Ocular Surface Damage
title_sort	<i>peucedanum japonicum</i> thunberg and its active components mitigate oxidative stress, inflammation and apoptosis after urban particulate matter-induced ocular surface damage
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0bc0e822718e4e1aa830f04b3c6453d8
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<i>Peucedanum japonicum</i> Thunberg and Its Active Components Mitigate Oxidative Stress, Inflammation and Apoptosis after Urban Particulate Matter-Induced Ocular Surface Damage

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