Improved methods for marking active neuron populations
Methods to directly label active neurons are still lacking. Here the authors develop CaMPARI2, a photoconvertible fluorescent protein sensor for neuronal activity with improved brightness and calcium binding kinetics, as well as an antibody to amplify the activated sensor signal in fixed samples.
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Nature Portfolio
2018
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| Acceso en línea: | https://doaj.org/article/0bc2383719ed4d3a919e09c4acecd7ca |
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oai:doaj.org-article:0bc2383719ed4d3a919e09c4acecd7ca2021-12-02T15:33:41ZImproved methods for marking active neuron populations10.1038/s41467-018-06935-22041-1723https://doaj.org/article/0bc2383719ed4d3a919e09c4acecd7ca2018-10-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-06935-2https://doaj.org/toc/2041-1723Methods to directly label active neurons are still lacking. Here the authors develop CaMPARI2, a photoconvertible fluorescent protein sensor for neuronal activity with improved brightness and calcium binding kinetics, as well as an antibody to amplify the activated sensor signal in fixed samples.Benjamien MoeyaertGraham HoltRajtarun MadangopalAlberto Perez-AlvarezBrenna C. FeareyNicholas F. TrojanowskiJulia LedderoseTimothy A. ZolnikAniruddha DasDavina PatelTimothy A. BrownRobert N. S. SachdevBritta J. EickholtMatthew E. LarkumGina G. TurrigianoHod DanaChristine E. GeeThomas G. OertnerBruce T. HopeEric R. SchreiterNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-12 (2018) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Science Q |
| spellingShingle |
Science Q Benjamien Moeyaert Graham Holt Rajtarun Madangopal Alberto Perez-Alvarez Brenna C. Fearey Nicholas F. Trojanowski Julia Ledderose Timothy A. Zolnik Aniruddha Das Davina Patel Timothy A. Brown Robert N. S. Sachdev Britta J. Eickholt Matthew E. Larkum Gina G. Turrigiano Hod Dana Christine E. Gee Thomas G. Oertner Bruce T. Hope Eric R. Schreiter Improved methods for marking active neuron populations |
| description |
Methods to directly label active neurons are still lacking. Here the authors develop CaMPARI2, a photoconvertible fluorescent protein sensor for neuronal activity with improved brightness and calcium binding kinetics, as well as an antibody to amplify the activated sensor signal in fixed samples. |
| format |
article |
| author |
Benjamien Moeyaert Graham Holt Rajtarun Madangopal Alberto Perez-Alvarez Brenna C. Fearey Nicholas F. Trojanowski Julia Ledderose Timothy A. Zolnik Aniruddha Das Davina Patel Timothy A. Brown Robert N. S. Sachdev Britta J. Eickholt Matthew E. Larkum Gina G. Turrigiano Hod Dana Christine E. Gee Thomas G. Oertner Bruce T. Hope Eric R. Schreiter |
| author_facet |
Benjamien Moeyaert Graham Holt Rajtarun Madangopal Alberto Perez-Alvarez Brenna C. Fearey Nicholas F. Trojanowski Julia Ledderose Timothy A. Zolnik Aniruddha Das Davina Patel Timothy A. Brown Robert N. S. Sachdev Britta J. Eickholt Matthew E. Larkum Gina G. Turrigiano Hod Dana Christine E. Gee Thomas G. Oertner Bruce T. Hope Eric R. Schreiter |
| author_sort |
Benjamien Moeyaert |
| title |
Improved methods for marking active neuron populations |
| title_short |
Improved methods for marking active neuron populations |
| title_full |
Improved methods for marking active neuron populations |
| title_fullStr |
Improved methods for marking active neuron populations |
| title_full_unstemmed |
Improved methods for marking active neuron populations |
| title_sort |
improved methods for marking active neuron populations |
| publisher |
Nature Portfolio |
| publishDate |
2018 |
| url |
https://doaj.org/article/0bc2383719ed4d3a919e09c4acecd7ca |
| work_keys_str_mv |
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