Improved methods for marking active neuron populations

Improved methods for marking active neuron populations

Methods to directly label active neurons are still lacking. Here the authors develop CaMPARI2, a photoconvertible fluorescent protein sensor for neuronal activity with improved brightness and calcium binding kinetics, as well as an antibody to amplify the activated sensor signal in fixed samples.

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Bibliographic Details
Main Authors: Benjamien Moeyaert, Graham Holt, Rajtarun Madangopal, Alberto Perez-Alvarez, Brenna C. Fearey, Nicholas F. Trojanowski, Julia Ledderose, Timothy A. Zolnik, Aniruddha Das, Davina Patel, Timothy A. Brown, Robert N. S. Sachdev, Britta J. Eickholt, Matthew E. Larkum, Gina G. Turrigiano, Hod Dana, Christine E. Gee, Thomas G. Oertner, Bruce T. Hope, Eric R. Schreiter
Format: article
Language:EN
Published: Nature Portfolio 2018
Subjects:
Science
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Online Access:https://doaj.org/article/0bc2383719ed4d3a919e09c4acecd7ca
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https://doaj.org/article/0bc2383719ed4d3a919e09c4acecd7ca

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