RANKL/OPG ratio regulates odontoclastogenesis in damaged dental pulp

Abstract Bone-resorbing osteoclasts are regulated by the relative ratio of the differentiation factor, receptor activator NF-kappa B ligand (RANKL) and its decoy receptor, osteoprotegerin (OPG). Dental tissue-localized-resorbing cells called odontoclasts have regulatory factors considered as identic...

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Autores principales:	Daisuke Nishida, Atsushi Arai, Lijuan Zhao, Mengyu Yang, Yuko Nakamichi, Kanji Horibe, Akihiro Hosoya, Yasuhiro Kobayashi, Nobuyuki Udagawa, Toshihide Mizoguchi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0bc798aa5f944ff192e4fdd53c5c0b34
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spelling	oai:doaj.org-article:0bc798aa5f944ff192e4fdd53c5c0b342021-12-02T13:35:04ZRANKL/OPG ratio regulates odontoclastogenesis in damaged dental pulp10.1038/s41598-021-84354-y2045-2322https://doaj.org/article/0bc798aa5f944ff192e4fdd53c5c0b342021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84354-yhttps://doaj.org/toc/2045-2322Abstract Bone-resorbing osteoclasts are regulated by the relative ratio of the differentiation factor, receptor activator NF-kappa B ligand (RANKL) and its decoy receptor, osteoprotegerin (OPG). Dental tissue-localized-resorbing cells called odontoclasts have regulatory factors considered as identical to those of osteoclasts; however, it is still unclear whether the RANKL/OPG ratio is a key factor for odontoclast regulation in dental pulp. Here, we showed that odontoclast regulators, macrophage colony-stimulating factor-1, RANKL, and OPG were detectable in mouse pulp of molars, but OPG was dominantly expressed. High OPG expression was expected to have a negative regulatory effect on odontoclastogenesis; however, odontoclasts were not detected in the dental pulp of OPG-deficient (KO) mice. In contrast, damage induced odontoclast-like cells were seen in wild-type pulp tissues, with their number significantly increased in OPG-KO mice. Relative ratio of RANKL/OPG in the damaged pulp was significantly higher than in undamaged control pulp. Pulp damages enhanced hypoxia inducible factor-1α and -2α, reported to increase RANKL or decrease OPG. These results reveal that the relative ratio of RANKL/OPG is significant to pulpal odontoclastogenesis, and that OPG expression is not required for maintenance of pulp homeostasis, but protects pulp from odontoclastogenesis caused by damages.Daisuke NishidaAtsushi AraiLijuan ZhaoMengyu YangYuko NakamichiKanji HoribeAkihiro HosoyaYasuhiro KobayashiNobuyuki UdagawaToshihide MizoguchiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daisuke Nishida Atsushi Arai Lijuan Zhao Mengyu Yang Yuko Nakamichi Kanji Horibe Akihiro Hosoya Yasuhiro Kobayashi Nobuyuki Udagawa Toshihide Mizoguchi RANKL/OPG ratio regulates odontoclastogenesis in damaged dental pulp
description	Abstract Bone-resorbing osteoclasts are regulated by the relative ratio of the differentiation factor, receptor activator NF-kappa B ligand (RANKL) and its decoy receptor, osteoprotegerin (OPG). Dental tissue-localized-resorbing cells called odontoclasts have regulatory factors considered as identical to those of osteoclasts; however, it is still unclear whether the RANKL/OPG ratio is a key factor for odontoclast regulation in dental pulp. Here, we showed that odontoclast regulators, macrophage colony-stimulating factor-1, RANKL, and OPG were detectable in mouse pulp of molars, but OPG was dominantly expressed. High OPG expression was expected to have a negative regulatory effect on odontoclastogenesis; however, odontoclasts were not detected in the dental pulp of OPG-deficient (KO) mice. In contrast, damage induced odontoclast-like cells were seen in wild-type pulp tissues, with their number significantly increased in OPG-KO mice. Relative ratio of RANKL/OPG in the damaged pulp was significantly higher than in undamaged control pulp. Pulp damages enhanced hypoxia inducible factor-1α and -2α, reported to increase RANKL or decrease OPG. These results reveal that the relative ratio of RANKL/OPG is significant to pulpal odontoclastogenesis, and that OPG expression is not required for maintenance of pulp homeostasis, but protects pulp from odontoclastogenesis caused by damages.
format	article
author	Daisuke Nishida Atsushi Arai Lijuan Zhao Mengyu Yang Yuko Nakamichi Kanji Horibe Akihiro Hosoya Yasuhiro Kobayashi Nobuyuki Udagawa Toshihide Mizoguchi
author_facet	Daisuke Nishida Atsushi Arai Lijuan Zhao Mengyu Yang Yuko Nakamichi Kanji Horibe Akihiro Hosoya Yasuhiro Kobayashi Nobuyuki Udagawa Toshihide Mizoguchi
author_sort	Daisuke Nishida
title	RANKL/OPG ratio regulates odontoclastogenesis in damaged dental pulp
title_short	RANKL/OPG ratio regulates odontoclastogenesis in damaged dental pulp
title_full	RANKL/OPG ratio regulates odontoclastogenesis in damaged dental pulp
title_fullStr	RANKL/OPG ratio regulates odontoclastogenesis in damaged dental pulp
title_full_unstemmed	RANKL/OPG ratio regulates odontoclastogenesis in damaged dental pulp
title_sort	rankl/opg ratio regulates odontoclastogenesis in damaged dental pulp
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0bc798aa5f944ff192e4fdd53c5c0b34
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RANKL/OPG ratio regulates odontoclastogenesis in damaged dental pulp

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