Comparative antler proteome of sika deer from different developmental stages

Abstract Antler is a special bone tissue that has the ability to regenerate completely periodically. It is the fastest growing bone in the animal kingdom. Antler provides a valuable research model for bone growth and mineralization. Antler grows longitudinally by endochondral ossification with their...

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Autores principales:	Ranran Zhang, Yang Li, Xiumei Xing
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0bcbe7f371a0491aac76d3462f368c14
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spelling	oai:doaj.org-article:0bcbe7f371a0491aac76d3462f368c142021-12-02T14:59:12ZComparative antler proteome of sika deer from different developmental stages10.1038/s41598-021-89829-62045-2322https://doaj.org/article/0bcbe7f371a0491aac76d3462f368c142021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89829-6https://doaj.org/toc/2045-2322Abstract Antler is a special bone tissue that has the ability to regenerate completely periodically. It is the fastest growing bone in the animal kingdom. Antler provides a valuable research model for bone growth and mineralization. Antler grows longitudinally by endochondral ossification with their growth center located in its tip. Many scholars have carried out detailed studies on morphology and gene expression of antler tip. However, few scholars have analyzed the protein expression patterns of antler tip at different development stages. This study used label-free proteomics approach to analyze the protein expression dynamics of the antler tip in six developmental periods (15, 25, 45, 65, 100 and 130 days after the previous antler cast) and costal cartilage. In result, 2052 proteins were confidently quantified, including 1937 antler proteins and 1044 costal cartilage proteins. Moreover, 913 antler core proteins and 132 antler-special proteins were obtained. Besides, the stages special proteins and differentially expressed proteins (DEPs) in different development stages were analyzed. A total of 875 DEPs were determined by one-way AVOVA. It is found that the growth period (15, 25, 45 and 65 days) showed more up-regulated protein including several chondrogenesis-associated proteins (collagen types II, collagen types XI, HAPLN1, PAPSS1 and PAPSS2). In ossification stages, the up-regulated proteins related with lysosome (CTSD, CTSB, MMP9, CAII) indicated that the antler has higher bone remodeling activity. Given the up-regulated expression of immune-related molecules (S100A7, CATHL7, LTF, AZU1, ELANE and MPO), we speculate that the local immune system may contribute to the ossification of antler tip. In conclusion, proteomics technology was used to deeply analyze the protein expression patterns of antler at different development stages. This provides a strong support for the research on the molecular regulation mechanism of rapid growth and ossification of velvet antler.Ranran ZhangYang LiXiumei XingNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ranran Zhang Yang Li Xiumei Xing Comparative antler proteome of sika deer from different developmental stages
description	Abstract Antler is a special bone tissue that has the ability to regenerate completely periodically. It is the fastest growing bone in the animal kingdom. Antler provides a valuable research model for bone growth and mineralization. Antler grows longitudinally by endochondral ossification with their growth center located in its tip. Many scholars have carried out detailed studies on morphology and gene expression of antler tip. However, few scholars have analyzed the protein expression patterns of antler tip at different development stages. This study used label-free proteomics approach to analyze the protein expression dynamics of the antler tip in six developmental periods (15, 25, 45, 65, 100 and 130 days after the previous antler cast) and costal cartilage. In result, 2052 proteins were confidently quantified, including 1937 antler proteins and 1044 costal cartilage proteins. Moreover, 913 antler core proteins and 132 antler-special proteins were obtained. Besides, the stages special proteins and differentially expressed proteins (DEPs) in different development stages were analyzed. A total of 875 DEPs were determined by one-way AVOVA. It is found that the growth period (15, 25, 45 and 65 days) showed more up-regulated protein including several chondrogenesis-associated proteins (collagen types II, collagen types XI, HAPLN1, PAPSS1 and PAPSS2). In ossification stages, the up-regulated proteins related with lysosome (CTSD, CTSB, MMP9, CAII) indicated that the antler has higher bone remodeling activity. Given the up-regulated expression of immune-related molecules (S100A7, CATHL7, LTF, AZU1, ELANE and MPO), we speculate that the local immune system may contribute to the ossification of antler tip. In conclusion, proteomics technology was used to deeply analyze the protein expression patterns of antler at different development stages. This provides a strong support for the research on the molecular regulation mechanism of rapid growth and ossification of velvet antler.
format	article
author	Ranran Zhang Yang Li Xiumei Xing
author_facet	Ranran Zhang Yang Li Xiumei Xing
author_sort	Ranran Zhang
title	Comparative antler proteome of sika deer from different developmental stages
title_short	Comparative antler proteome of sika deer from different developmental stages
title_full	Comparative antler proteome of sika deer from different developmental stages
title_fullStr	Comparative antler proteome of sika deer from different developmental stages
title_full_unstemmed	Comparative antler proteome of sika deer from different developmental stages
title_sort	comparative antler proteome of sika deer from different developmental stages
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0bcbe7f371a0491aac76d3462f368c14
work_keys_str_mv	AT ranranzhang comparativeantlerproteomeofsikadeerfromdifferentdevelopmentalstages AT yangli comparativeantlerproteomeofsikadeerfromdifferentdevelopmentalstages AT xiumeixing comparativeantlerproteomeofsikadeerfromdifferentdevelopmentalstages
_version_	1718389182501486592

Comparative antler proteome of sika deer from different developmental stages

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