Genome editing in primary cells and in vivo using viral-derived Nanoblades loaded with Cas9-sgRNA ribonucleoproteins
A current challenge in genome editing is delivering Cas9 and sgRNA into target cells. Here the authors engineer a delivery system based on murine leukemia virus-like particles loaded with Cas9-sgRNA ribonucleoproteins to induce efficient genome editing in both cell culture and in vivo in mouse.
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Nature Portfolio
2019
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oai:doaj.org-article:0bd13889c5db4e0ba26c33892287ae452021-12-02T14:40:01ZGenome editing in primary cells and in vivo using viral-derived Nanoblades loaded with Cas9-sgRNA ribonucleoproteins10.1038/s41467-018-07845-z2041-1723https://doaj.org/article/0bd13889c5db4e0ba26c33892287ae452019-01-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-07845-zhttps://doaj.org/toc/2041-1723A current challenge in genome editing is delivering Cas9 and sgRNA into target cells. Here the authors engineer a delivery system based on murine leukemia virus-like particles loaded with Cas9-sgRNA ribonucleoproteins to induce efficient genome editing in both cell culture and in vivo in mouse.Philippe E. MangeotValérie RissonFloriane FusilAline MarnefEmilie LaurentJuliana BlinVirginie MournetasEmmanuelle MassouridèsThibault J. M. SohierAntoine CorbinFabien AubéMarie TeixeiraChristian PinsetLaurent SchaefferGaëlle LegubeFrançois-Loïc CossetEls VerhoeyenThéophile OhlmannEmiliano P. RicciNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-15 (2019) |
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EN |
| topic |
Science Q |
| spellingShingle |
Science Q Philippe E. Mangeot Valérie Risson Floriane Fusil Aline Marnef Emilie Laurent Juliana Blin Virginie Mournetas Emmanuelle Massouridès Thibault J. M. Sohier Antoine Corbin Fabien Aubé Marie Teixeira Christian Pinset Laurent Schaeffer Gaëlle Legube François-Loïc Cosset Els Verhoeyen Théophile Ohlmann Emiliano P. Ricci Genome editing in primary cells and in vivo using viral-derived Nanoblades loaded with Cas9-sgRNA ribonucleoproteins |
| description |
A current challenge in genome editing is delivering Cas9 and sgRNA into target cells. Here the authors engineer a delivery system based on murine leukemia virus-like particles loaded with Cas9-sgRNA ribonucleoproteins to induce efficient genome editing in both cell culture and in vivo in mouse. |
| format |
article |
| author |
Philippe E. Mangeot Valérie Risson Floriane Fusil Aline Marnef Emilie Laurent Juliana Blin Virginie Mournetas Emmanuelle Massouridès Thibault J. M. Sohier Antoine Corbin Fabien Aubé Marie Teixeira Christian Pinset Laurent Schaeffer Gaëlle Legube François-Loïc Cosset Els Verhoeyen Théophile Ohlmann Emiliano P. Ricci |
| author_facet |
Philippe E. Mangeot Valérie Risson Floriane Fusil Aline Marnef Emilie Laurent Juliana Blin Virginie Mournetas Emmanuelle Massouridès Thibault J. M. Sohier Antoine Corbin Fabien Aubé Marie Teixeira Christian Pinset Laurent Schaeffer Gaëlle Legube François-Loïc Cosset Els Verhoeyen Théophile Ohlmann Emiliano P. Ricci |
| author_sort |
Philippe E. Mangeot |
| title |
Genome editing in primary cells and in vivo using viral-derived Nanoblades loaded with Cas9-sgRNA ribonucleoproteins |
| title_short |
Genome editing in primary cells and in vivo using viral-derived Nanoblades loaded with Cas9-sgRNA ribonucleoproteins |
| title_full |
Genome editing in primary cells and in vivo using viral-derived Nanoblades loaded with Cas9-sgRNA ribonucleoproteins |
| title_fullStr |
Genome editing in primary cells and in vivo using viral-derived Nanoblades loaded with Cas9-sgRNA ribonucleoproteins |
| title_full_unstemmed |
Genome editing in primary cells and in vivo using viral-derived Nanoblades loaded with Cas9-sgRNA ribonucleoproteins |
| title_sort |
genome editing in primary cells and in vivo using viral-derived nanoblades loaded with cas9-sgrna ribonucleoproteins |
| publisher |
Nature Portfolio |
| publishDate |
2019 |
| url |
https://doaj.org/article/0bd13889c5db4e0ba26c33892287ae45 |
| work_keys_str_mv |
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