A-RAF kinase functions in ARF6 regulated endocytic membrane traffic.

<h4>Background</h4>RAF kinases direct ERK MAPK signaling to distinct subcellular compartments in response to growth factor stimulation.<h4>Methodology/principal findings</h4>Of the three mammalian isoforms A-RAF is special in that one of its two lipid binding domains mediates...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Elena Nekhoroshkova, Stefan Albert, Matthias Becker, Ulf R Rapp
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0bdac0f91c3c4d48afbb205d0c60fa7e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0bdac0f91c3c4d48afbb205d0c60fa7e
record_format	dspace
spelling	oai:doaj.org-article:0bdac0f91c3c4d48afbb205d0c60fa7e2021-11-25T06:17:02ZA-RAF kinase functions in ARF6 regulated endocytic membrane traffic.1932-620310.1371/journal.pone.0004647https://doaj.org/article/0bdac0f91c3c4d48afbb205d0c60fa7e2009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19247477/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>RAF kinases direct ERK MAPK signaling to distinct subcellular compartments in response to growth factor stimulation.<h4>Methodology/principal findings</h4>Of the three mammalian isoforms A-RAF is special in that one of its two lipid binding domains mediates a unique pattern of membrane localization. Specific membrane binding is retained by an N-terminal fragment (AR149) that corresponds to a naturally occurring splice variant termed DA-RAF2. AR149 colocalizes with ARF6 on tubular endosomes and has a dominant negative effect on endocytic trafficking. Moreover actin polymerization of yeast and mammalian cells is abolished. AR149/DA-RAF2 does not affect the internalization step of endocytosis, but trafficking to the recycling compartment.<h4>Conclusions/significance</h4>A-RAF induced ERK activation is required for this step by activating ARF6, as A-RAF depletion or inhibition of the A-RAF controlled MEK-ERK cascade blocks recycling. These data led to a new model for A-RAF function in endocytic trafficking.Elena NekhoroshkovaStefan AlbertMatthias BeckerUlf R RappPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 2, p e4647 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elena Nekhoroshkova Stefan Albert Matthias Becker Ulf R Rapp A-RAF kinase functions in ARF6 regulated endocytic membrane traffic.
description	<h4>Background</h4>RAF kinases direct ERK MAPK signaling to distinct subcellular compartments in response to growth factor stimulation.<h4>Methodology/principal findings</h4>Of the three mammalian isoforms A-RAF is special in that one of its two lipid binding domains mediates a unique pattern of membrane localization. Specific membrane binding is retained by an N-terminal fragment (AR149) that corresponds to a naturally occurring splice variant termed DA-RAF2. AR149 colocalizes with ARF6 on tubular endosomes and has a dominant negative effect on endocytic trafficking. Moreover actin polymerization of yeast and mammalian cells is abolished. AR149/DA-RAF2 does not affect the internalization step of endocytosis, but trafficking to the recycling compartment.<h4>Conclusions/significance</h4>A-RAF induced ERK activation is required for this step by activating ARF6, as A-RAF depletion or inhibition of the A-RAF controlled MEK-ERK cascade blocks recycling. These data led to a new model for A-RAF function in endocytic trafficking.
format	article
author	Elena Nekhoroshkova Stefan Albert Matthias Becker Ulf R Rapp
author_facet	Elena Nekhoroshkova Stefan Albert Matthias Becker Ulf R Rapp
author_sort	Elena Nekhoroshkova
title	A-RAF kinase functions in ARF6 regulated endocytic membrane traffic.
title_short	A-RAF kinase functions in ARF6 regulated endocytic membrane traffic.
title_full	A-RAF kinase functions in ARF6 regulated endocytic membrane traffic.
title_fullStr	A-RAF kinase functions in ARF6 regulated endocytic membrane traffic.
title_full_unstemmed	A-RAF kinase functions in ARF6 regulated endocytic membrane traffic.
title_sort	a-raf kinase functions in arf6 regulated endocytic membrane traffic.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/0bdac0f91c3c4d48afbb205d0c60fa7e
work_keys_str_mv	AT elenanekhoroshkova arafkinasefunctionsinarf6regulatedendocyticmembranetraffic AT stefanalbert arafkinasefunctionsinarf6regulatedendocyticmembranetraffic AT matthiasbecker arafkinasefunctionsinarf6regulatedendocyticmembranetraffic AT ulfrrapp arafkinasefunctionsinarf6regulatedendocyticmembranetraffic
_version_	1718413965512409088

A-RAF kinase functions in ARF6 regulated endocytic membrane traffic.

Ejemplares similares