Endometriosis foci differentiation by rapid lipid profiling using tissue spray ionization and high resolution mass spectrometry

Abstract Obtaining fast screening information on molecular composition of a tissue sample is of great importance for a disease biomarkers search and for online surgery control. In this study, high resolution mass spectrometry analysis of eutopic and ectopic endometrium tissues (90 samples) is done u...

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Autores principales: Vitaliy V. Chagovets, Zhihao Wang, Alexey S. Kononikhin, Natalia L. Starodubtseva, Anna Borisova, Dinara Salimova, Igor A. Popov, Andrey V. Kozachenko, Konstantin Chingin, Huanwen Chen, Vladimir E. Frankevich, Leila V. Adamyan, Gennady T. Sukhikh
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Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/0bdb5d6429df4946a048d6213e48a3b9
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spelling oai:doaj.org-article:0bdb5d6429df4946a048d6213e48a3b92021-12-02T16:06:33ZEndometriosis foci differentiation by rapid lipid profiling using tissue spray ionization and high resolution mass spectrometry10.1038/s41598-017-02708-x2045-2322https://doaj.org/article/0bdb5d6429df4946a048d6213e48a3b92017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02708-xhttps://doaj.org/toc/2045-2322Abstract Obtaining fast screening information on molecular composition of a tissue sample is of great importance for a disease biomarkers search and for online surgery control. In this study, high resolution mass spectrometry analysis of eutopic and ectopic endometrium tissues (90 samples) is done using direct tissue spray mass spectrometry in both positive and negative ion modes. The most abundant peaks in the both ion modes are those corresponding to lipids. Species of three lipid classes are observed, phosphatidylcholines (PC), sphingomyelins (SM) and phosphoethanolamines (PE). Direct tissue analysis gives mainly information on PC and SM lipids (29 species) in positive ion mode and PC, SM and PE lipids (50 species) in negative ion mode which gives complementary data for endometriosis foci differentiation. The biggest differences were found for phospholipids with polyunsaturated acyls and alkils. Although, tissue spray shows itself as appropriate tool for tissue investigation, caution should be paid to the interpretation of mass spectra because of their higher complexity with more possible adducts formation and multiple interferences must be taken into account. The present work extends the application of direct tissue analysis for the rapid differentiation between endometriotic tissues of different foci.Vitaliy V. ChagovetsZhihao WangAlexey S. KononikhinNatalia L. StarodubtsevaAnna BorisovaDinara SalimovaIgor A. PopovAndrey V. KozachenkoKonstantin ChinginHuanwen ChenVladimir E. FrankevichLeila V. AdamyanGennady T. SukhikhNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Vitaliy V. Chagovets
Zhihao Wang
Alexey S. Kononikhin
Natalia L. Starodubtseva
Anna Borisova
Dinara Salimova
Igor A. Popov
Andrey V. Kozachenko
Konstantin Chingin
Huanwen Chen
Vladimir E. Frankevich
Leila V. Adamyan
Gennady T. Sukhikh
Endometriosis foci differentiation by rapid lipid profiling using tissue spray ionization and high resolution mass spectrometry
description Abstract Obtaining fast screening information on molecular composition of a tissue sample is of great importance for a disease biomarkers search and for online surgery control. In this study, high resolution mass spectrometry analysis of eutopic and ectopic endometrium tissues (90 samples) is done using direct tissue spray mass spectrometry in both positive and negative ion modes. The most abundant peaks in the both ion modes are those corresponding to lipids. Species of three lipid classes are observed, phosphatidylcholines (PC), sphingomyelins (SM) and phosphoethanolamines (PE). Direct tissue analysis gives mainly information on PC and SM lipids (29 species) in positive ion mode and PC, SM and PE lipids (50 species) in negative ion mode which gives complementary data for endometriosis foci differentiation. The biggest differences were found for phospholipids with polyunsaturated acyls and alkils. Although, tissue spray shows itself as appropriate tool for tissue investigation, caution should be paid to the interpretation of mass spectra because of their higher complexity with more possible adducts formation and multiple interferences must be taken into account. The present work extends the application of direct tissue analysis for the rapid differentiation between endometriotic tissues of different foci.
format article
author Vitaliy V. Chagovets
Zhihao Wang
Alexey S. Kononikhin
Natalia L. Starodubtseva
Anna Borisova
Dinara Salimova
Igor A. Popov
Andrey V. Kozachenko
Konstantin Chingin
Huanwen Chen
Vladimir E. Frankevich
Leila V. Adamyan
Gennady T. Sukhikh
author_facet Vitaliy V. Chagovets
Zhihao Wang
Alexey S. Kononikhin
Natalia L. Starodubtseva
Anna Borisova
Dinara Salimova
Igor A. Popov
Andrey V. Kozachenko
Konstantin Chingin
Huanwen Chen
Vladimir E. Frankevich
Leila V. Adamyan
Gennady T. Sukhikh
author_sort Vitaliy V. Chagovets
title Endometriosis foci differentiation by rapid lipid profiling using tissue spray ionization and high resolution mass spectrometry
title_short Endometriosis foci differentiation by rapid lipid profiling using tissue spray ionization and high resolution mass spectrometry
title_full Endometriosis foci differentiation by rapid lipid profiling using tissue spray ionization and high resolution mass spectrometry
title_fullStr Endometriosis foci differentiation by rapid lipid profiling using tissue spray ionization and high resolution mass spectrometry
title_full_unstemmed Endometriosis foci differentiation by rapid lipid profiling using tissue spray ionization and high resolution mass spectrometry
title_sort endometriosis foci differentiation by rapid lipid profiling using tissue spray ionization and high resolution mass spectrometry
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/0bdb5d6429df4946a048d6213e48a3b9
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