Endometriosis foci differentiation by rapid lipid profiling using tissue spray ionization and high resolution mass spectrometry
Abstract Obtaining fast screening information on molecular composition of a tissue sample is of great importance for a disease biomarkers search and for online surgery control. In this study, high resolution mass spectrometry analysis of eutopic and ectopic endometrium tissues (90 samples) is done u...
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Nature Portfolio
2017
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oai:doaj.org-article:0bdb5d6429df4946a048d6213e48a3b92021-12-02T16:06:33ZEndometriosis foci differentiation by rapid lipid profiling using tissue spray ionization and high resolution mass spectrometry10.1038/s41598-017-02708-x2045-2322https://doaj.org/article/0bdb5d6429df4946a048d6213e48a3b92017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02708-xhttps://doaj.org/toc/2045-2322Abstract Obtaining fast screening information on molecular composition of a tissue sample is of great importance for a disease biomarkers search and for online surgery control. In this study, high resolution mass spectrometry analysis of eutopic and ectopic endometrium tissues (90 samples) is done using direct tissue spray mass spectrometry in both positive and negative ion modes. The most abundant peaks in the both ion modes are those corresponding to lipids. Species of three lipid classes are observed, phosphatidylcholines (PC), sphingomyelins (SM) and phosphoethanolamines (PE). Direct tissue analysis gives mainly information on PC and SM lipids (29 species) in positive ion mode and PC, SM and PE lipids (50 species) in negative ion mode which gives complementary data for endometriosis foci differentiation. The biggest differences were found for phospholipids with polyunsaturated acyls and alkils. Although, tissue spray shows itself as appropriate tool for tissue investigation, caution should be paid to the interpretation of mass spectra because of their higher complexity with more possible adducts formation and multiple interferences must be taken into account. The present work extends the application of direct tissue analysis for the rapid differentiation between endometriotic tissues of different foci.Vitaliy V. ChagovetsZhihao WangAlexey S. KononikhinNatalia L. StarodubtsevaAnna BorisovaDinara SalimovaIgor A. PopovAndrey V. KozachenkoKonstantin ChinginHuanwen ChenVladimir E. FrankevichLeila V. AdamyanGennady T. SukhikhNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017) |
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Medicine R Science Q Vitaliy V. Chagovets Zhihao Wang Alexey S. Kononikhin Natalia L. Starodubtseva Anna Borisova Dinara Salimova Igor A. Popov Andrey V. Kozachenko Konstantin Chingin Huanwen Chen Vladimir E. Frankevich Leila V. Adamyan Gennady T. Sukhikh Endometriosis foci differentiation by rapid lipid profiling using tissue spray ionization and high resolution mass spectrometry |
| description |
Abstract Obtaining fast screening information on molecular composition of a tissue sample is of great importance for a disease biomarkers search and for online surgery control. In this study, high resolution mass spectrometry analysis of eutopic and ectopic endometrium tissues (90 samples) is done using direct tissue spray mass spectrometry in both positive and negative ion modes. The most abundant peaks in the both ion modes are those corresponding to lipids. Species of three lipid classes are observed, phosphatidylcholines (PC), sphingomyelins (SM) and phosphoethanolamines (PE). Direct tissue analysis gives mainly information on PC and SM lipids (29 species) in positive ion mode and PC, SM and PE lipids (50 species) in negative ion mode which gives complementary data for endometriosis foci differentiation. The biggest differences were found for phospholipids with polyunsaturated acyls and alkils. Although, tissue spray shows itself as appropriate tool for tissue investigation, caution should be paid to the interpretation of mass spectra because of their higher complexity with more possible adducts formation and multiple interferences must be taken into account. The present work extends the application of direct tissue analysis for the rapid differentiation between endometriotic tissues of different foci. |
| format |
article |
| author |
Vitaliy V. Chagovets Zhihao Wang Alexey S. Kononikhin Natalia L. Starodubtseva Anna Borisova Dinara Salimova Igor A. Popov Andrey V. Kozachenko Konstantin Chingin Huanwen Chen Vladimir E. Frankevich Leila V. Adamyan Gennady T. Sukhikh |
| author_facet |
Vitaliy V. Chagovets Zhihao Wang Alexey S. Kononikhin Natalia L. Starodubtseva Anna Borisova Dinara Salimova Igor A. Popov Andrey V. Kozachenko Konstantin Chingin Huanwen Chen Vladimir E. Frankevich Leila V. Adamyan Gennady T. Sukhikh |
| author_sort |
Vitaliy V. Chagovets |
| title |
Endometriosis foci differentiation by rapid lipid profiling using tissue spray ionization and high resolution mass spectrometry |
| title_short |
Endometriosis foci differentiation by rapid lipid profiling using tissue spray ionization and high resolution mass spectrometry |
| title_full |
Endometriosis foci differentiation by rapid lipid profiling using tissue spray ionization and high resolution mass spectrometry |
| title_fullStr |
Endometriosis foci differentiation by rapid lipid profiling using tissue spray ionization and high resolution mass spectrometry |
| title_full_unstemmed |
Endometriosis foci differentiation by rapid lipid profiling using tissue spray ionization and high resolution mass spectrometry |
| title_sort |
endometriosis foci differentiation by rapid lipid profiling using tissue spray ionization and high resolution mass spectrometry |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/0bdb5d6429df4946a048d6213e48a3b9 |
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