A practical method for storage, preservation and transportation of anuran urine samples using filter paper for hormone analysis

Anurans (frogs and toads) expelled urine when handled and it could provide insights into their physiological status. However, storage, preservation and transportation are often challenging. The study aimed to standardize and validate a field method for short-term storage and preserve of anuran urine...

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Autores principales:	Vinod Kumar, Shudhanta Sood, Karthikeyan Vasudevan, Govindhaswamy Umapathy
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	A field storage method of anuran urine on filter paper for hormone analysis Science Q
Acceso en línea:	https://doaj.org/article/0bdcc7d3db30486aac803f2ea5da56d9
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spelling	oai:doaj.org-article:0bdcc7d3db30486aac803f2ea5da56d92021-11-18T04:49:11ZA practical method for storage, preservation and transportation of anuran urine samples using filter paper for hormone analysis2215-016110.1016/j.mex.2021.101578https://doaj.org/article/0bdcc7d3db30486aac803f2ea5da56d92021-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S221501612100368Xhttps://doaj.org/toc/2215-0161Anurans (frogs and toads) expelled urine when handled and it could provide insights into their physiological status. However, storage, preservation and transportation are often challenging. The study aimed to standardize and validate a field method for short-term storage and preserve of anuran urine samples using Whatman filter papers. To examine the efficacy of storage conditions and type of papers, urinary based enzyme immunoassays were used to measure progesterone and testosterone hormone metabolites. High-Performance Liquid Chromatography was performed and revealed immunoreactive progesterone and testosterone metabolites in the urine samples. Urinary hormone metabolites concentration stored in filter paper at room temperature and control samples stored in -20°C for the same period were similar. Whatman grade 50 was found to be more suitable for storage of hormones than grade 3 paper for the experiments performed. • A cheap and simple storage method for storage of anuran urine in field conditions using filter papers. • Anuran urine could be preserved and transported under ambient conditions without significant changes and loss of hormones. • This method would facilitate the endocrine monitoring of anurans in remote areas where limited logistics are available.Vinod KumarShudhanta SoodKarthikeyan VasudevanGovindhaswamy UmapathyElsevierarticleA field storage method of anuran urine on filter paper for hormone analysisScienceQENMethodsX, Vol 8, Iss , Pp 101578- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	A field storage method of anuran urine on filter paper for hormone analysis Science Q
spellingShingle	A field storage method of anuran urine on filter paper for hormone analysis Science Q Vinod Kumar Shudhanta Sood Karthikeyan Vasudevan Govindhaswamy Umapathy A practical method for storage, preservation and transportation of anuran urine samples using filter paper for hormone analysis
description	Anurans (frogs and toads) expelled urine when handled and it could provide insights into their physiological status. However, storage, preservation and transportation are often challenging. The study aimed to standardize and validate a field method for short-term storage and preserve of anuran urine samples using Whatman filter papers. To examine the efficacy of storage conditions and type of papers, urinary based enzyme immunoassays were used to measure progesterone and testosterone hormone metabolites. High-Performance Liquid Chromatography was performed and revealed immunoreactive progesterone and testosterone metabolites in the urine samples. Urinary hormone metabolites concentration stored in filter paper at room temperature and control samples stored in -20°C for the same period were similar. Whatman grade 50 was found to be more suitable for storage of hormones than grade 3 paper for the experiments performed. • A cheap and simple storage method for storage of anuran urine in field conditions using filter papers. • Anuran urine could be preserved and transported under ambient conditions without significant changes and loss of hormones. • This method would facilitate the endocrine monitoring of anurans in remote areas where limited logistics are available.
format	article
author	Vinod Kumar Shudhanta Sood Karthikeyan Vasudevan Govindhaswamy Umapathy
author_facet	Vinod Kumar Shudhanta Sood Karthikeyan Vasudevan Govindhaswamy Umapathy
author_sort	Vinod Kumar
title	A practical method for storage, preservation and transportation of anuran urine samples using filter paper for hormone analysis
title_short	A practical method for storage, preservation and transportation of anuran urine samples using filter paper for hormone analysis
title_full	A practical method for storage, preservation and transportation of anuran urine samples using filter paper for hormone analysis
title_fullStr	A practical method for storage, preservation and transportation of anuran urine samples using filter paper for hormone analysis
title_full_unstemmed	A practical method for storage, preservation and transportation of anuran urine samples using filter paper for hormone analysis
title_sort	practical method for storage, preservation and transportation of anuran urine samples using filter paper for hormone analysis
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/0bdcc7d3db30486aac803f2ea5da56d9
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A practical method for storage, preservation and transportation of anuran urine samples using filter paper for hormone analysis

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