Bio-printing of aligned GelMa-based cell-laden structure for muscle tissue regeneration

Volumetric muscle loss (VML) is associated with a severe loss of muscle tissue that overwhelms the regenerative potential of skeletal muscles. Tissue engineering has shown promise for the treatment of VML injuries, as evidenced by various preclinical trials. The present study describes the fabricati...

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Autores principales:	Hanjun Hwangbo, Hyeongjin Lee, Eun-Ju Jin, JaeYoon Lee, Yunju Jo, Dongryeol Ryu, GeunHyung Kim
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2022
Materias:	GelMa bio-ink in-situ crosslinking 3D bioprinting Myogenesis Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/0bdd43887c5c4c3c8ef2f9fa36dbfa57
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spelling	oai:doaj.org-article:0bdd43887c5c4c3c8ef2f9fa36dbfa572021-11-04T04:36:10ZBio-printing of aligned GelMa-based cell-laden structure for muscle tissue regeneration2452-199X10.1016/j.bioactmat.2021.06.031https://doaj.org/article/0bdd43887c5c4c3c8ef2f9fa36dbfa572022-02-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2452199X21003170https://doaj.org/toc/2452-199XVolumetric muscle loss (VML) is associated with a severe loss of muscle tissue that overwhelms the regenerative potential of skeletal muscles. Tissue engineering has shown promise for the treatment of VML injuries, as evidenced by various preclinical trials. The present study describes the fabrication of a cell-laden GelMa muscle construct using an in situ crosslinking (ISC) strategy to improve muscle functionality. To obtain optimal biophysical properties of the muscle construct, two UV exposure sources, UV exposure dose, and wall shear stress were evaluated using C2C12 myoblasts. Additionally, the ISC system showed a significantly higher degree of uniaxial alignment and myogenesis compared to the conventional crosslinking strategy (post-crosslinking). To evaluate the in vivo regenerative potential, muscle constructs laden with human adipose stem cells were used. The VML defect group implanted with the bio-printed muscle construct showed significant restoration of functionality and muscular volume. The data presented in this study suggest that stem cell-based therapies combined with the modified bioprinting process could potentially be effective against VML injuries.Hanjun HwangboHyeongjin LeeEun-Ju JinJaeYoon LeeYunju JoDongryeol RyuGeunHyung KimKeAi Communications Co., Ltd.articleGelMa bio-inkin-situ crosslinking3D bioprintingMyogenesisMaterials of engineering and construction. Mechanics of materialsTA401-492Biology (General)QH301-705.5ENBioactive Materials, Vol 8, Iss , Pp 57-70 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	GelMa bio-ink in-situ crosslinking 3D bioprinting Myogenesis Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
spellingShingle	GelMa bio-ink in-situ crosslinking 3D bioprinting Myogenesis Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5 Hanjun Hwangbo Hyeongjin Lee Eun-Ju Jin JaeYoon Lee Yunju Jo Dongryeol Ryu GeunHyung Kim Bio-printing of aligned GelMa-based cell-laden structure for muscle tissue regeneration
description	Volumetric muscle loss (VML) is associated with a severe loss of muscle tissue that overwhelms the regenerative potential of skeletal muscles. Tissue engineering has shown promise for the treatment of VML injuries, as evidenced by various preclinical trials. The present study describes the fabrication of a cell-laden GelMa muscle construct using an in situ crosslinking (ISC) strategy to improve muscle functionality. To obtain optimal biophysical properties of the muscle construct, two UV exposure sources, UV exposure dose, and wall shear stress were evaluated using C2C12 myoblasts. Additionally, the ISC system showed a significantly higher degree of uniaxial alignment and myogenesis compared to the conventional crosslinking strategy (post-crosslinking). To evaluate the in vivo regenerative potential, muscle constructs laden with human adipose stem cells were used. The VML defect group implanted with the bio-printed muscle construct showed significant restoration of functionality and muscular volume. The data presented in this study suggest that stem cell-based therapies combined with the modified bioprinting process could potentially be effective against VML injuries.
format	article
author	Hanjun Hwangbo Hyeongjin Lee Eun-Ju Jin JaeYoon Lee Yunju Jo Dongryeol Ryu GeunHyung Kim
author_facet	Hanjun Hwangbo Hyeongjin Lee Eun-Ju Jin JaeYoon Lee Yunju Jo Dongryeol Ryu GeunHyung Kim
author_sort	Hanjun Hwangbo
title	Bio-printing of aligned GelMa-based cell-laden structure for muscle tissue regeneration
title_short	Bio-printing of aligned GelMa-based cell-laden structure for muscle tissue regeneration
title_full	Bio-printing of aligned GelMa-based cell-laden structure for muscle tissue regeneration
title_fullStr	Bio-printing of aligned GelMa-based cell-laden structure for muscle tissue regeneration
title_full_unstemmed	Bio-printing of aligned GelMa-based cell-laden structure for muscle tissue regeneration
title_sort	bio-printing of aligned gelma-based cell-laden structure for muscle tissue regeneration
publisher	KeAi Communications Co., Ltd.
publishDate	2022
url	https://doaj.org/article/0bdd43887c5c4c3c8ef2f9fa36dbfa57
work_keys_str_mv	AT hanjunhwangbo bioprintingofalignedgelmabasedcellladenstructureformuscletissueregeneration AT hyeongjinlee bioprintingofalignedgelmabasedcellladenstructureformuscletissueregeneration AT eunjujin bioprintingofalignedgelmabasedcellladenstructureformuscletissueregeneration AT jaeyoonlee bioprintingofalignedgelmabasedcellladenstructureformuscletissueregeneration AT yunjujo bioprintingofalignedgelmabasedcellladenstructureformuscletissueregeneration AT dongryeolryu bioprintingofalignedgelmabasedcellladenstructureformuscletissueregeneration AT geunhyungkim bioprintingofalignedgelmabasedcellladenstructureformuscletissueregeneration
_version_	1718445252936728576

Bio-printing of aligned GelMa-based cell-laden structure for muscle tissue regeneration

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