Tissue-specific alteration of gene expression and function by RU486 and the GeneSwitch system

Abstract The GeneSwitch (GS) is a modified Gal4/UAS system, whereby transgene expression is induced in Drosophila by adding the drug RU486 to food. The GS system is routinely used in Drosophila aging and behavioral studies to avoid confounding effects related to genetic background mutations. Here, w...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Maricela Robles-Murguia, Liam C. Hunt, David Finkelstein, Yiping Fan, Fabio Demontis
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Geriatrics RC952-954.6
Acceso en línea:	https://doaj.org/article/0bdd8edebade4c678cefaaed563f6f11
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0bdd8edebade4c678cefaaed563f6f11
record_format	dspace
spelling	oai:doaj.org-article:0bdd8edebade4c678cefaaed563f6f112021-12-02T16:05:45ZTissue-specific alteration of gene expression and function by RU486 and the GeneSwitch system10.1038/s41514-019-0036-82056-3973https://doaj.org/article/0bdd8edebade4c678cefaaed563f6f112019-05-01T00:00:00Zhttps://doi.org/10.1038/s41514-019-0036-8https://doaj.org/toc/2056-3973Abstract The GeneSwitch (GS) is a modified Gal4/UAS system, whereby transgene expression is induced in Drosophila by adding the drug RU486 to food. The GS system is routinely used in Drosophila aging and behavioral studies to avoid confounding effects related to genetic background mutations. Here, we report transcriptional and functional defects that are induced by RU486 in a stock- and tissue-dependent manner, such as defects in flight and mitochondrial gene expression. In addition to including proper controls, our findings suggest that context-specific side effects induced by RU486 should be considered in the experimental design and when interpreting the observed phenotypes.Maricela Robles-MurguiaLiam C. HuntDavid FinkelsteinYiping FanFabio DemontisNature PortfolioarticleGeriatricsRC952-954.6ENnpj Aging and Mechanisms of Disease, Vol 5, Iss 1, Pp 1-5 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Geriatrics RC952-954.6
spellingShingle	Geriatrics RC952-954.6 Maricela Robles-Murguia Liam C. Hunt David Finkelstein Yiping Fan Fabio Demontis Tissue-specific alteration of gene expression and function by RU486 and the GeneSwitch system
description	Abstract The GeneSwitch (GS) is a modified Gal4/UAS system, whereby transgene expression is induced in Drosophila by adding the drug RU486 to food. The GS system is routinely used in Drosophila aging and behavioral studies to avoid confounding effects related to genetic background mutations. Here, we report transcriptional and functional defects that are induced by RU486 in a stock- and tissue-dependent manner, such as defects in flight and mitochondrial gene expression. In addition to including proper controls, our findings suggest that context-specific side effects induced by RU486 should be considered in the experimental design and when interpreting the observed phenotypes.
format	article
author	Maricela Robles-Murguia Liam C. Hunt David Finkelstein Yiping Fan Fabio Demontis
author_facet	Maricela Robles-Murguia Liam C. Hunt David Finkelstein Yiping Fan Fabio Demontis
author_sort	Maricela Robles-Murguia
title	Tissue-specific alteration of gene expression and function by RU486 and the GeneSwitch system
title_short	Tissue-specific alteration of gene expression and function by RU486 and the GeneSwitch system
title_full	Tissue-specific alteration of gene expression and function by RU486 and the GeneSwitch system
title_fullStr	Tissue-specific alteration of gene expression and function by RU486 and the GeneSwitch system
title_full_unstemmed	Tissue-specific alteration of gene expression and function by RU486 and the GeneSwitch system
title_sort	tissue-specific alteration of gene expression and function by ru486 and the geneswitch system
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/0bdd8edebade4c678cefaaed563f6f11
work_keys_str_mv	AT maricelaroblesmurguia tissuespecificalterationofgeneexpressionandfunctionbyru486andthegeneswitchsystem AT liamchunt tissuespecificalterationofgeneexpressionandfunctionbyru486andthegeneswitchsystem AT davidfinkelstein tissuespecificalterationofgeneexpressionandfunctionbyru486andthegeneswitchsystem AT yipingfan tissuespecificalterationofgeneexpressionandfunctionbyru486andthegeneswitchsystem AT fabiodemontis tissuespecificalterationofgeneexpressionandfunctionbyru486andthegeneswitchsystem
_version_	1718385129001320448

Tissue-specific alteration of gene expression and function by RU486 and the GeneSwitch system

Ejemplares similares