A single reporter mouse line for Vika, Flp, Dre, and Cre-recombination

Abstract Site-specific recombinases (SSR) are utilized as important genome engineering tools to precisely modify the genome of mice and other model organisms. Reporter mice that mark cells that at any given time had expressed the enzyme are frequently used for lineage tracing and to characterize new...

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Autores principales:	Madina Karimova, Oliver Baker, Aylin Camgoz, Ronald Naumann, Frank Buchholz, Konstantinos Anastassiadis
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Reporter Mice Viking Recombination Target Sites Conditional Mutagenesis Recombinase System Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0bdfdff459e04664809de4437ac08886
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spelling	oai:doaj.org-article:0bdfdff459e04664809de4437ac088862021-12-02T11:40:17ZA single reporter mouse line for Vika, Flp, Dre, and Cre-recombination10.1038/s41598-018-32802-72045-2322https://doaj.org/article/0bdfdff459e04664809de4437ac088862018-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-32802-7https://doaj.org/toc/2045-2322Abstract Site-specific recombinases (SSR) are utilized as important genome engineering tools to precisely modify the genome of mice and other model organisms. Reporter mice that mark cells that at any given time had expressed the enzyme are frequently used for lineage tracing and to characterize newly generated mice expressing a recombinase from a chosen promoter. With increasing sophistication of genome alteration strategies, the demand for novel SSR systems that efficiently and specifically recombine their targets is rising and several SSR-systems are now used in combination to address complex biological questions in vivo. Generation of reporter mice for each one of these recombinases is cumbersome and increases the number of mouse lines that need to be maintained in animal facilities. Here we present a multi-reporter mouse line for loci-of-recombination (X) (MuX) that streamlines the characterization of mice expressing prominent recombinases. MuX mice constitutively express nuclear green fluorescent protein after recombination by either Cre, Flp, Dre or Vika recombinase, rationalizing the number of animal lines that need to be maintained. We also pioneer the use of the Vika/vox system in mice, illustrating its high efficacy and specificity, thereby facilitating future designs of sophisticated recombinase-based in vivo genome engineering strategies.Madina KarimovaOliver BakerAylin CamgozRonald NaumannFrank BuchholzKonstantinos AnastassiadisNature PortfolioarticleReporter MiceVikingRecombination Target SitesConditional MutagenesisRecombinase SystemMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Reporter Mice Viking Recombination Target Sites Conditional Mutagenesis Recombinase System Medicine R Science Q
spellingShingle	Reporter Mice Viking Recombination Target Sites Conditional Mutagenesis Recombinase System Medicine R Science Q Madina Karimova Oliver Baker Aylin Camgoz Ronald Naumann Frank Buchholz Konstantinos Anastassiadis A single reporter mouse line for Vika, Flp, Dre, and Cre-recombination
description	Abstract Site-specific recombinases (SSR) are utilized as important genome engineering tools to precisely modify the genome of mice and other model organisms. Reporter mice that mark cells that at any given time had expressed the enzyme are frequently used for lineage tracing and to characterize newly generated mice expressing a recombinase from a chosen promoter. With increasing sophistication of genome alteration strategies, the demand for novel SSR systems that efficiently and specifically recombine their targets is rising and several SSR-systems are now used in combination to address complex biological questions in vivo. Generation of reporter mice for each one of these recombinases is cumbersome and increases the number of mouse lines that need to be maintained in animal facilities. Here we present a multi-reporter mouse line for loci-of-recombination (X) (MuX) that streamlines the characterization of mice expressing prominent recombinases. MuX mice constitutively express nuclear green fluorescent protein after recombination by either Cre, Flp, Dre or Vika recombinase, rationalizing the number of animal lines that need to be maintained. We also pioneer the use of the Vika/vox system in mice, illustrating its high efficacy and specificity, thereby facilitating future designs of sophisticated recombinase-based in vivo genome engineering strategies.
format	article
author	Madina Karimova Oliver Baker Aylin Camgoz Ronald Naumann Frank Buchholz Konstantinos Anastassiadis
author_facet	Madina Karimova Oliver Baker Aylin Camgoz Ronald Naumann Frank Buchholz Konstantinos Anastassiadis
author_sort	Madina Karimova
title	A single reporter mouse line for Vika, Flp, Dre, and Cre-recombination
title_short	A single reporter mouse line for Vika, Flp, Dre, and Cre-recombination
title_full	A single reporter mouse line for Vika, Flp, Dre, and Cre-recombination
title_fullStr	A single reporter mouse line for Vika, Flp, Dre, and Cre-recombination
title_full_unstemmed	A single reporter mouse line for Vika, Flp, Dre, and Cre-recombination
title_sort	single reporter mouse line for vika, flp, dre, and cre-recombination
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/0bdfdff459e04664809de4437ac08886
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A single reporter mouse line for Vika, Flp, Dre, and Cre-recombination

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