Degradation of antibiotic resistance genes and mobile gene elements in dairy manure anerobic digestion.

Antibiotic resistance genes (ARGs) are emerging contaminants causing serious global health concern. Interventions to address this concern include improving our understanding of methods for treating waste material of human and animal origin that are known to harbor ARGs. Anaerobic digestion is a comm...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yi Wang, Pramod K Pandey, Sundaram Kuppu, Richard Pereira, Sharif Aly, Ruihong Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0bec752c2a0f4936974751af6cebb028
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0bec752c2a0f4936974751af6cebb028
record_format	dspace
spelling	oai:doaj.org-article:0bec752c2a0f4936974751af6cebb0282021-12-02T20:17:36ZDegradation of antibiotic resistance genes and mobile gene elements in dairy manure anerobic digestion.1932-620310.1371/journal.pone.0254836https://doaj.org/article/0bec752c2a0f4936974751af6cebb0282021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0254836https://doaj.org/toc/1932-6203Antibiotic resistance genes (ARGs) are emerging contaminants causing serious global health concern. Interventions to address this concern include improving our understanding of methods for treating waste material of human and animal origin that are known to harbor ARGs. Anaerobic digestion is a commonly used process for treating dairy manure, and although effective in reducing ARGs, its mechanism of action is not clear. In this study, we used three ARGs to conducted a longitudinal bench scale anaerobic digestion experiment with various temperatures (28, 36, 44, and 52°C) in triplicate using fresh dairy manure for 30 days to evaluate the reduction of gene abundance. Three ARGs and two mobile genetic elements (MGEs) were studied: sulfonamide resistance gene (sulII), tetracycline resistance genes (tetW), macrolide-lincosamide-streptogramin B (MLSB) superfamily resistance genes (ermF), class 1 integrase gene (intI1), and transposase gene (tnpA). Genes were quantified by real-time quantitative PCR. Results show that the thermophilic anaerobic digestion (52°C) significantly reduced (p < 0.05) the absolute abundance of sulII (95%), intI1 (95%), tnpA (77%) and 16S rRNA gene (76%) after 30 days of digestion. A modified Collins-Selleck model was used to fit the decay curve, and results suggest that the gene reduction during the startup phase of anaerobic digestion (first 5 days) was faster than the later stage, and reductions in the first five days were more than 50% for most genes.Yi WangPramod K PandeySundaram KuppuRichard PereiraSharif AlyRuihong ZhangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0254836 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yi Wang Pramod K Pandey Sundaram Kuppu Richard Pereira Sharif Aly Ruihong Zhang Degradation of antibiotic resistance genes and mobile gene elements in dairy manure anerobic digestion.
description	Antibiotic resistance genes (ARGs) are emerging contaminants causing serious global health concern. Interventions to address this concern include improving our understanding of methods for treating waste material of human and animal origin that are known to harbor ARGs. Anaerobic digestion is a commonly used process for treating dairy manure, and although effective in reducing ARGs, its mechanism of action is not clear. In this study, we used three ARGs to conducted a longitudinal bench scale anaerobic digestion experiment with various temperatures (28, 36, 44, and 52°C) in triplicate using fresh dairy manure for 30 days to evaluate the reduction of gene abundance. Three ARGs and two mobile genetic elements (MGEs) were studied: sulfonamide resistance gene (sulII), tetracycline resistance genes (tetW), macrolide-lincosamide-streptogramin B (MLSB) superfamily resistance genes (ermF), class 1 integrase gene (intI1), and transposase gene (tnpA). Genes were quantified by real-time quantitative PCR. Results show that the thermophilic anaerobic digestion (52°C) significantly reduced (p < 0.05) the absolute abundance of sulII (95%), intI1 (95%), tnpA (77%) and 16S rRNA gene (76%) after 30 days of digestion. A modified Collins-Selleck model was used to fit the decay curve, and results suggest that the gene reduction during the startup phase of anaerobic digestion (first 5 days) was faster than the later stage, and reductions in the first five days were more than 50% for most genes.
format	article
author	Yi Wang Pramod K Pandey Sundaram Kuppu Richard Pereira Sharif Aly Ruihong Zhang
author_facet	Yi Wang Pramod K Pandey Sundaram Kuppu Richard Pereira Sharif Aly Ruihong Zhang
author_sort	Yi Wang
title	Degradation of antibiotic resistance genes and mobile gene elements in dairy manure anerobic digestion.
title_short	Degradation of antibiotic resistance genes and mobile gene elements in dairy manure anerobic digestion.
title_full	Degradation of antibiotic resistance genes and mobile gene elements in dairy manure anerobic digestion.
title_fullStr	Degradation of antibiotic resistance genes and mobile gene elements in dairy manure anerobic digestion.
title_full_unstemmed	Degradation of antibiotic resistance genes and mobile gene elements in dairy manure anerobic digestion.
title_sort	degradation of antibiotic resistance genes and mobile gene elements in dairy manure anerobic digestion.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/0bec752c2a0f4936974751af6cebb028
work_keys_str_mv	AT yiwang degradationofantibioticresistancegenesandmobilegeneelementsindairymanureanerobicdigestion AT pramodkpandey degradationofantibioticresistancegenesandmobilegeneelementsindairymanureanerobicdigestion AT sundaramkuppu degradationofantibioticresistancegenesandmobilegeneelementsindairymanureanerobicdigestion AT richardpereira degradationofantibioticresistancegenesandmobilegeneelementsindairymanureanerobicdigestion AT sharifaly degradationofantibioticresistancegenesandmobilegeneelementsindairymanureanerobicdigestion AT ruihongzhang degradationofantibioticresistancegenesandmobilegeneelementsindairymanureanerobicdigestion
_version_	1718374327961780224

Degradation of antibiotic resistance genes and mobile gene elements in dairy manure anerobic digestion.

Ejemplares similares