Multiwell Raman plate reader for high-throughput biochemical screening

Abstract Although Raman spectroscopy has been used for the quantitative analysis of samples in many fields, including material science, biomedical, and pharmaceutical research, its low sensitivity hindered the application of the analytical capability for high-throughput screening. Here, we developed...

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Autores principales:	Hiroyuki Kawagoe, Jun Ando, Miwako Asanuma, Kosuke Dodo, Tetsuya Miyano, Hiroshi Ueda, Mikiko Sodeoka, Katsumasa Fujita
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0bed3504a3f24681aa5102ba1de8b4b7
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spelling	oai:doaj.org-article:0bed3504a3f24681aa5102ba1de8b4b72021-12-02T17:06:24ZMultiwell Raman plate reader for high-throughput biochemical screening10.1038/s41598-021-95139-82045-2322https://doaj.org/article/0bed3504a3f24681aa5102ba1de8b4b72021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95139-8https://doaj.org/toc/2045-2322Abstract Although Raman spectroscopy has been used for the quantitative analysis of samples in many fields, including material science, biomedical, and pharmaceutical research, its low sensitivity hindered the application of the analytical capability for high-throughput screening. Here, we developed a high-throughput Raman screening system that can analyze hundreds of specimens in a multiwell plate simultaneously. Multiple high numerical aperture (NA) lenses are assembled under each well in the multiwell plate to detect Raman scattering simultaneously with high sensitivity. The Raman spectrum of 192 samples loaded on a standard 384-well plate can be analyzed simultaneously. With the developed system, the throughput of Raman measurement was significantly improved (about 100 times) compared to conventional Raman instruments based on a single-point measurement. By using the developed system, we demonstrated high-throughput Raman screening to investigate drug polymorphism and identify a small-molecule binding site in a protein. Furthermore, the same system was used to demonstrate high-speed chemical mapping of a centimeter-sized pork slice.Hiroyuki KawagoeJun AndoMiwako AsanumaKosuke DodoTetsuya MiyanoHiroshi UedaMikiko SodeokaKatsumasa FujitaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hiroyuki Kawagoe Jun Ando Miwako Asanuma Kosuke Dodo Tetsuya Miyano Hiroshi Ueda Mikiko Sodeoka Katsumasa Fujita Multiwell Raman plate reader for high-throughput biochemical screening
description	Abstract Although Raman spectroscopy has been used for the quantitative analysis of samples in many fields, including material science, biomedical, and pharmaceutical research, its low sensitivity hindered the application of the analytical capability for high-throughput screening. Here, we developed a high-throughput Raman screening system that can analyze hundreds of specimens in a multiwell plate simultaneously. Multiple high numerical aperture (NA) lenses are assembled under each well in the multiwell plate to detect Raman scattering simultaneously with high sensitivity. The Raman spectrum of 192 samples loaded on a standard 384-well plate can be analyzed simultaneously. With the developed system, the throughput of Raman measurement was significantly improved (about 100 times) compared to conventional Raman instruments based on a single-point measurement. By using the developed system, we demonstrated high-throughput Raman screening to investigate drug polymorphism and identify a small-molecule binding site in a protein. Furthermore, the same system was used to demonstrate high-speed chemical mapping of a centimeter-sized pork slice.
format	article
author	Hiroyuki Kawagoe Jun Ando Miwako Asanuma Kosuke Dodo Tetsuya Miyano Hiroshi Ueda Mikiko Sodeoka Katsumasa Fujita
author_facet	Hiroyuki Kawagoe Jun Ando Miwako Asanuma Kosuke Dodo Tetsuya Miyano Hiroshi Ueda Mikiko Sodeoka Katsumasa Fujita
author_sort	Hiroyuki Kawagoe
title	Multiwell Raman plate reader for high-throughput biochemical screening
title_short	Multiwell Raman plate reader for high-throughput biochemical screening
title_full	Multiwell Raman plate reader for high-throughput biochemical screening
title_fullStr	Multiwell Raman plate reader for high-throughput biochemical screening
title_full_unstemmed	Multiwell Raman plate reader for high-throughput biochemical screening
title_sort	multiwell raman plate reader for high-throughput biochemical screening
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0bed3504a3f24681aa5102ba1de8b4b7
work_keys_str_mv	AT hiroyukikawagoe multiwellramanplatereaderforhighthroughputbiochemicalscreening AT junando multiwellramanplatereaderforhighthroughputbiochemicalscreening AT miwakoasanuma multiwellramanplatereaderforhighthroughputbiochemicalscreening AT kosukedodo multiwellramanplatereaderforhighthroughputbiochemicalscreening AT tetsuyamiyano multiwellramanplatereaderforhighthroughputbiochemicalscreening AT hiroshiueda multiwellramanplatereaderforhighthroughputbiochemicalscreening AT mikikosodeoka multiwellramanplatereaderforhighthroughputbiochemicalscreening AT katsumasafujita multiwellramanplatereaderforhighthroughputbiochemicalscreening
_version_	1718381596892987392

Multiwell Raman plate reader for high-throughput biochemical screening

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