Staphylococcus aureus Lipase 3 (SAL3) is a surface-associated lipase that hydrolyzes short chain fatty acids.

Bacterial lipases play important roles during infection. The Staphylococcus aureus genome contains several genes that encode well-characterized lipases and several genes predicted to encode lipases or esterases for which the function has not yet been established. In this study, we sought to define t...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Naren Gajenthra Kumar, Daniel Contaifer, Dayanjan S Wijesinghe, Kimberly K Jefferson
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2021
Materias:
R
Q
Acceso en línea:https://doaj.org/article/0bf3e1dac19a4929918bae8626e4d564
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:0bf3e1dac19a4929918bae8626e4d564
record_format dspace
spelling oai:doaj.org-article:0bf3e1dac19a4929918bae8626e4d5642021-12-02T20:17:12ZStaphylococcus aureus Lipase 3 (SAL3) is a surface-associated lipase that hydrolyzes short chain fatty acids.1932-620310.1371/journal.pone.0258106https://doaj.org/article/0bf3e1dac19a4929918bae8626e4d5642021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0258106https://doaj.org/toc/1932-6203Bacterial lipases play important roles during infection. The Staphylococcus aureus genome contains several genes that encode well-characterized lipases and several genes predicted to encode lipases or esterases for which the function has not yet been established. In this study, we sought to define the function of an uncharacterized S. aureus protein, and we propose the annotation S. aureus lipase 3 (SAL3) (SAUSA300_0641). We confirmed that SAL3 is a lipase and that it is surface associated and secreted through an unknown mechanism. We determined that SAL3 specifically hydrolyzes short chain (4-carbon and fewer) fatty acids and specifically binds negatively charged lipids including phosphatidic acid, phosphatidylinositol phosphate, and phosphatidylglycerol, which is the most abundant lipid in the staphylococcal cell membrane. Mutating the catalytic triad S66-A, D167-A, S168-A, and H301-A in the recombinant protein abolished lipase activity without altering binding to host lipid substrates. Taken together we report the discovery of a novel lipase from S. aureus specific to short chain fatty acids with yet to be determined roles in host pathogen interactions.Naren Gajenthra KumarDaniel ContaiferDayanjan S WijesingheKimberly K JeffersonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10, p e0258106 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Naren Gajenthra Kumar
Daniel Contaifer
Dayanjan S Wijesinghe
Kimberly K Jefferson
Staphylococcus aureus Lipase 3 (SAL3) is a surface-associated lipase that hydrolyzes short chain fatty acids.
description Bacterial lipases play important roles during infection. The Staphylococcus aureus genome contains several genes that encode well-characterized lipases and several genes predicted to encode lipases or esterases for which the function has not yet been established. In this study, we sought to define the function of an uncharacterized S. aureus protein, and we propose the annotation S. aureus lipase 3 (SAL3) (SAUSA300_0641). We confirmed that SAL3 is a lipase and that it is surface associated and secreted through an unknown mechanism. We determined that SAL3 specifically hydrolyzes short chain (4-carbon and fewer) fatty acids and specifically binds negatively charged lipids including phosphatidic acid, phosphatidylinositol phosphate, and phosphatidylglycerol, which is the most abundant lipid in the staphylococcal cell membrane. Mutating the catalytic triad S66-A, D167-A, S168-A, and H301-A in the recombinant protein abolished lipase activity without altering binding to host lipid substrates. Taken together we report the discovery of a novel lipase from S. aureus specific to short chain fatty acids with yet to be determined roles in host pathogen interactions.
format article
author Naren Gajenthra Kumar
Daniel Contaifer
Dayanjan S Wijesinghe
Kimberly K Jefferson
author_facet Naren Gajenthra Kumar
Daniel Contaifer
Dayanjan S Wijesinghe
Kimberly K Jefferson
author_sort Naren Gajenthra Kumar
title Staphylococcus aureus Lipase 3 (SAL3) is a surface-associated lipase that hydrolyzes short chain fatty acids.
title_short Staphylococcus aureus Lipase 3 (SAL3) is a surface-associated lipase that hydrolyzes short chain fatty acids.
title_full Staphylococcus aureus Lipase 3 (SAL3) is a surface-associated lipase that hydrolyzes short chain fatty acids.
title_fullStr Staphylococcus aureus Lipase 3 (SAL3) is a surface-associated lipase that hydrolyzes short chain fatty acids.
title_full_unstemmed Staphylococcus aureus Lipase 3 (SAL3) is a surface-associated lipase that hydrolyzes short chain fatty acids.
title_sort staphylococcus aureus lipase 3 (sal3) is a surface-associated lipase that hydrolyzes short chain fatty acids.
publisher Public Library of Science (PLoS)
publishDate 2021
url https://doaj.org/article/0bf3e1dac19a4929918bae8626e4d564
work_keys_str_mv AT narengajenthrakumar staphylococcusaureuslipase3sal3isasurfaceassociatedlipasethathydrolyzesshortchainfattyacids
AT danielcontaifer staphylococcusaureuslipase3sal3isasurfaceassociatedlipasethathydrolyzesshortchainfattyacids
AT dayanjanswijesinghe staphylococcusaureuslipase3sal3isasurfaceassociatedlipasethathydrolyzesshortchainfattyacids
AT kimberlykjefferson staphylococcusaureuslipase3sal3isasurfaceassociatedlipasethathydrolyzesshortchainfattyacids
_version_ 1718374388299988992