Autologous culture method improves retention of tumors’ native properties

Abstract No current in vitro tumor model replicates a tumor’s in vivo microenvironment. A culturing technique that better preserves a tumor’s pathophysiological conditions is needed for some important clinical applications, including personalized drug-sensitivity/resistance assays. In this study, we...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yao Tang, Qian Xu, Meiling Yan, Yimin Zhang, Ping Zhu, Xianghong Li, Limin Sang, Ming Zhang, Wenhe Huang, Lianxing Lin, Jundong Wu, Yue Xin, Junhui Fu, Li Zhang, Shuming Zhang, Jiang Gu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0bfa3c25d3e04fc6816c771667b65652
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0bfa3c25d3e04fc6816c771667b65652
record_format	dspace
spelling	oai:doaj.org-article:0bfa3c25d3e04fc6816c771667b656522021-12-02T15:10:31ZAutologous culture method improves retention of tumors’ native properties10.1038/s41598-020-77238-02045-2322https://doaj.org/article/0bfa3c25d3e04fc6816c771667b656522020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77238-0https://doaj.org/toc/2045-2322Abstract No current in vitro tumor model replicates a tumor’s in vivo microenvironment. A culturing technique that better preserves a tumor’s pathophysiological conditions is needed for some important clinical applications, including personalized drug-sensitivity/resistance assays. In this study, we utilized autologous serum or body fluid to build a 3D scaffold and grow a patient’s tumor. We named this technique “3D-ACM” (autologous culture method). Forty-five clinical samples from biopsies, surgically removed tumor tissues and malignant body fluids were cultured with 3D-ACM. Traditional 3D-FBS (fetal bovine serum) cultures were performed side-by-side for comparison. The results were that cells cultured in 3D-ACM rebuilt tissue-like structures, and retained their immuno-phenotypes and cytokine productions. In contrast, the 3D-FBS method promoted mesenchymal cell proliferation. In preliminary chemo drug-sensitivity assays, significantly higher mortality was always associated with FBS-cultured cells. Accordingly, 3D-ACM appears to more reliably preserve a tumor’s biological characteristics, which might improve the accuracy of drug-testing for personalized cancer treatment.Yao TangQian XuMeiling YanYimin ZhangPing ZhuXianghong LiLimin SangMing ZhangWenhe HuangLianxing LinJundong WuYue XinJunhui FuLi ZhangShuming ZhangJiang GuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yao Tang Qian Xu Meiling Yan Yimin Zhang Ping Zhu Xianghong Li Limin Sang Ming Zhang Wenhe Huang Lianxing Lin Jundong Wu Yue Xin Junhui Fu Li Zhang Shuming Zhang Jiang Gu Autologous culture method improves retention of tumors’ native properties
description	Abstract No current in vitro tumor model replicates a tumor’s in vivo microenvironment. A culturing technique that better preserves a tumor’s pathophysiological conditions is needed for some important clinical applications, including personalized drug-sensitivity/resistance assays. In this study, we utilized autologous serum or body fluid to build a 3D scaffold and grow a patient’s tumor. We named this technique “3D-ACM” (autologous culture method). Forty-five clinical samples from biopsies, surgically removed tumor tissues and malignant body fluids were cultured with 3D-ACM. Traditional 3D-FBS (fetal bovine serum) cultures were performed side-by-side for comparison. The results were that cells cultured in 3D-ACM rebuilt tissue-like structures, and retained their immuno-phenotypes and cytokine productions. In contrast, the 3D-FBS method promoted mesenchymal cell proliferation. In preliminary chemo drug-sensitivity assays, significantly higher mortality was always associated with FBS-cultured cells. Accordingly, 3D-ACM appears to more reliably preserve a tumor’s biological characteristics, which might improve the accuracy of drug-testing for personalized cancer treatment.
format	article
author	Yao Tang Qian Xu Meiling Yan Yimin Zhang Ping Zhu Xianghong Li Limin Sang Ming Zhang Wenhe Huang Lianxing Lin Jundong Wu Yue Xin Junhui Fu Li Zhang Shuming Zhang Jiang Gu
author_facet	Yao Tang Qian Xu Meiling Yan Yimin Zhang Ping Zhu Xianghong Li Limin Sang Ming Zhang Wenhe Huang Lianxing Lin Jundong Wu Yue Xin Junhui Fu Li Zhang Shuming Zhang Jiang Gu
author_sort	Yao Tang
title	Autologous culture method improves retention of tumors’ native properties
title_short	Autologous culture method improves retention of tumors’ native properties
title_full	Autologous culture method improves retention of tumors’ native properties
title_fullStr	Autologous culture method improves retention of tumors’ native properties
title_full_unstemmed	Autologous culture method improves retention of tumors’ native properties
title_sort	autologous culture method improves retention of tumors’ native properties
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/0bfa3c25d3e04fc6816c771667b65652
work_keys_str_mv	AT yaotang autologousculturemethodimprovesretentionoftumorsnativeproperties AT qianxu autologousculturemethodimprovesretentionoftumorsnativeproperties AT meilingyan autologousculturemethodimprovesretentionoftumorsnativeproperties AT yiminzhang autologousculturemethodimprovesretentionoftumorsnativeproperties AT pingzhu autologousculturemethodimprovesretentionoftumorsnativeproperties AT xianghongli autologousculturemethodimprovesretentionoftumorsnativeproperties AT liminsang autologousculturemethodimprovesretentionoftumorsnativeproperties AT mingzhang autologousculturemethodimprovesretentionoftumorsnativeproperties AT wenhehuang autologousculturemethodimprovesretentionoftumorsnativeproperties AT lianxinglin autologousculturemethodimprovesretentionoftumorsnativeproperties AT jundongwu autologousculturemethodimprovesretentionoftumorsnativeproperties AT yuexin autologousculturemethodimprovesretentionoftumorsnativeproperties AT junhuifu autologousculturemethodimprovesretentionoftumorsnativeproperties AT lizhang autologousculturemethodimprovesretentionoftumorsnativeproperties AT shumingzhang autologousculturemethodimprovesretentionoftumorsnativeproperties AT jianggu autologousculturemethodimprovesretentionoftumorsnativeproperties
_version_	1718387673642565632

Autologous culture method improves retention of tumors’ native properties

Ejemplares similares