Gene expression analysis of Alcaligenes faecalis during induction of heterotrophic nitrification

Abstract Alcaligenes faecalis is a heterotrophic nitrifying bacterium that oxidizes ammonia and generates nitrite and nitrate. When A. faecalis was cultivated in a medium containing pyruvate and ammonia as the sole carbon and nitrogen sources, respectively, high concentrations of nitrite accumulated...

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Autores principales:	Shuhei Tsujino, Hideo Dohra, Taketomo Fujiwara
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0c03f55e9d9e46e3ad8350039c22365e
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spelling	oai:doaj.org-article:0c03f55e9d9e46e3ad8350039c22365e2021-12-05T12:13:05ZGene expression analysis of Alcaligenes faecalis during induction of heterotrophic nitrification10.1038/s41598-021-02579-32045-2322https://doaj.org/article/0c03f55e9d9e46e3ad8350039c22365e2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02579-3https://doaj.org/toc/2045-2322Abstract Alcaligenes faecalis is a heterotrophic nitrifying bacterium that oxidizes ammonia and generates nitrite and nitrate. When A. faecalis was cultivated in a medium containing pyruvate and ammonia as the sole carbon and nitrogen sources, respectively, high concentrations of nitrite accumulated in the medium whose carbon/nitrogen (C/N) ratio was lower than 10 during the exponential growth phase, while the accumulation was not observed in the medium whose C/N ratio was higher than 15. Comparative transcriptome analysis was performed using nitrifying and non-nitrifying cells of A. faecalis cultivated in media whose C/N ratios were 5 and 20, respectively, to evaluate the fluctuations of gene expression during induction of heterotrophic nitrification. Expression levels of genes involved in primary metabolism did not change significantly in the cells at the exponential growth phase under both conditions. We observed a significant increase in the expression levels of four gene clusters: pod cluster containing the gene encoding pyruvic oxime dioxygenase (POD), podh cluster containing the gene encoding a POD homolog (PODh), suf cluster involved in an iron-sulfur cluster biogenesis, and dnf cluster involved in a novel hydroxylamine oxidation pathway in the nitrifying cells. Our results provide valuable insight into the biochemical mechanism of heterotrophic nitrification.Shuhei TsujinoHideo DohraTaketomo FujiwaraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shuhei Tsujino Hideo Dohra Taketomo Fujiwara Gene expression analysis of Alcaligenes faecalis during induction of heterotrophic nitrification
description	Abstract Alcaligenes faecalis is a heterotrophic nitrifying bacterium that oxidizes ammonia and generates nitrite and nitrate. When A. faecalis was cultivated in a medium containing pyruvate and ammonia as the sole carbon and nitrogen sources, respectively, high concentrations of nitrite accumulated in the medium whose carbon/nitrogen (C/N) ratio was lower than 10 during the exponential growth phase, while the accumulation was not observed in the medium whose C/N ratio was higher than 15. Comparative transcriptome analysis was performed using nitrifying and non-nitrifying cells of A. faecalis cultivated in media whose C/N ratios were 5 and 20, respectively, to evaluate the fluctuations of gene expression during induction of heterotrophic nitrification. Expression levels of genes involved in primary metabolism did not change significantly in the cells at the exponential growth phase under both conditions. We observed a significant increase in the expression levels of four gene clusters: pod cluster containing the gene encoding pyruvic oxime dioxygenase (POD), podh cluster containing the gene encoding a POD homolog (PODh), suf cluster involved in an iron-sulfur cluster biogenesis, and dnf cluster involved in a novel hydroxylamine oxidation pathway in the nitrifying cells. Our results provide valuable insight into the biochemical mechanism of heterotrophic nitrification.
format	article
author	Shuhei Tsujino Hideo Dohra Taketomo Fujiwara
author_facet	Shuhei Tsujino Hideo Dohra Taketomo Fujiwara
author_sort	Shuhei Tsujino
title	Gene expression analysis of Alcaligenes faecalis during induction of heterotrophic nitrification
title_short	Gene expression analysis of Alcaligenes faecalis during induction of heterotrophic nitrification
title_full	Gene expression analysis of Alcaligenes faecalis during induction of heterotrophic nitrification
title_fullStr	Gene expression analysis of Alcaligenes faecalis during induction of heterotrophic nitrification
title_full_unstemmed	Gene expression analysis of Alcaligenes faecalis during induction of heterotrophic nitrification
title_sort	gene expression analysis of alcaligenes faecalis during induction of heterotrophic nitrification
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0c03f55e9d9e46e3ad8350039c22365e
work_keys_str_mv	AT shuheitsujino geneexpressionanalysisofalcaligenesfaecalisduringinductionofheterotrophicnitrification AT hideodohra geneexpressionanalysisofalcaligenesfaecalisduringinductionofheterotrophicnitrification AT taketomofujiwara geneexpressionanalysisofalcaligenesfaecalisduringinductionofheterotrophicnitrification
_version_	1718372116060962816

Gene expression analysis of Alcaligenes faecalis during induction of heterotrophic nitrification

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