Frequent and efficient use of the sister chromatid for DNA double-strand break repair during budding yeast meiosis.

Recombination between homologous chromosomes of different parental origin (homologs) is necessary for their accurate segregation during meiosis. It has been suggested that meiotic inter-homolog recombination is promoted by a barrier to inter-sister-chromatid recombination, imposed by meiosis-specifi...

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Autores principales:	Tamara Goldfarb, Michael Lichten
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/0c09380a1985438594dbdf334eafaa2b
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spelling	oai:doaj.org-article:0c09380a1985438594dbdf334eafaa2b2021-11-18T05:36:29ZFrequent and efficient use of the sister chromatid for DNA double-strand break repair during budding yeast meiosis.1544-91731545-788510.1371/journal.pbio.1000520https://doaj.org/article/0c09380a1985438594dbdf334eafaa2b2010-10-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20976044/pdf/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Recombination between homologous chromosomes of different parental origin (homologs) is necessary for their accurate segregation during meiosis. It has been suggested that meiotic inter-homolog recombination is promoted by a barrier to inter-sister-chromatid recombination, imposed by meiosis-specific components of the chromosome axis. Consistent with this, measures of Holliday junction-containing recombination intermediates (joint molecules [JMs]) show a strong bias towards inter-homolog and against inter-sister JMs. However, recombination between sister chromatids also has an important role in meiosis. The genomes of diploid organisms in natural populations are highly polymorphic for insertions and deletions, and meiotic double-strand breaks (DSBs) that form within such polymorphic regions must be repaired by inter-sister recombination. Efforts to study inter-sister recombination during meiosis, in particular to determine recombination frequencies and mechanisms, have been constrained by the inability to monitor the products of inter-sister recombination. We present here molecular-level studies of inter-sister recombination during budding yeast meiosis. We examined events initiated by DSBs in regions that lack corresponding sequences on the homolog, and show that these DSBs are efficiently repaired by inter-sister recombination. This occurs with the same timing as inter-homolog recombination, but with reduced (2- to 3-fold) yields of JMs. Loss of the meiotic-chromosome-axis-associated kinase Mek1 accelerates inter-sister DSB repair and markedly increases inter-sister JM frequencies. Furthermore, inter-sister JMs formed in mek1Δ mutants are preferentially lost, while inter-homolog JMs are maintained. These findings indicate that inter-sister recombination occurs frequently during budding yeast meiosis, with the possibility that up to one-third of all recombination events occur between sister chromatids. We suggest that a Mek1-dependent reduction in the rate of inter-sister repair, combined with the destabilization of inter-sister JMs, promotes inter-homolog recombination while retaining the capacity for inter-sister recombination when inter-homolog recombination is not possible.Tamara GoldfarbMichael LichtenPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 8, Iss 10, p e1000520 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Tamara Goldfarb Michael Lichten Frequent and efficient use of the sister chromatid for DNA double-strand break repair during budding yeast meiosis.
description	Recombination between homologous chromosomes of different parental origin (homologs) is necessary for their accurate segregation during meiosis. It has been suggested that meiotic inter-homolog recombination is promoted by a barrier to inter-sister-chromatid recombination, imposed by meiosis-specific components of the chromosome axis. Consistent with this, measures of Holliday junction-containing recombination intermediates (joint molecules [JMs]) show a strong bias towards inter-homolog and against inter-sister JMs. However, recombination between sister chromatids also has an important role in meiosis. The genomes of diploid organisms in natural populations are highly polymorphic for insertions and deletions, and meiotic double-strand breaks (DSBs) that form within such polymorphic regions must be repaired by inter-sister recombination. Efforts to study inter-sister recombination during meiosis, in particular to determine recombination frequencies and mechanisms, have been constrained by the inability to monitor the products of inter-sister recombination. We present here molecular-level studies of inter-sister recombination during budding yeast meiosis. We examined events initiated by DSBs in regions that lack corresponding sequences on the homolog, and show that these DSBs are efficiently repaired by inter-sister recombination. This occurs with the same timing as inter-homolog recombination, but with reduced (2- to 3-fold) yields of JMs. Loss of the meiotic-chromosome-axis-associated kinase Mek1 accelerates inter-sister DSB repair and markedly increases inter-sister JM frequencies. Furthermore, inter-sister JMs formed in mek1Δ mutants are preferentially lost, while inter-homolog JMs are maintained. These findings indicate that inter-sister recombination occurs frequently during budding yeast meiosis, with the possibility that up to one-third of all recombination events occur between sister chromatids. We suggest that a Mek1-dependent reduction in the rate of inter-sister repair, combined with the destabilization of inter-sister JMs, promotes inter-homolog recombination while retaining the capacity for inter-sister recombination when inter-homolog recombination is not possible.
format	article
author	Tamara Goldfarb Michael Lichten
author_facet	Tamara Goldfarb Michael Lichten
author_sort	Tamara Goldfarb
title	Frequent and efficient use of the sister chromatid for DNA double-strand break repair during budding yeast meiosis.
title_short	Frequent and efficient use of the sister chromatid for DNA double-strand break repair during budding yeast meiosis.
title_full	Frequent and efficient use of the sister chromatid for DNA double-strand break repair during budding yeast meiosis.
title_fullStr	Frequent and efficient use of the sister chromatid for DNA double-strand break repair during budding yeast meiosis.
title_full_unstemmed	Frequent and efficient use of the sister chromatid for DNA double-strand break repair during budding yeast meiosis.
title_sort	frequent and efficient use of the sister chromatid for dna double-strand break repair during budding yeast meiosis.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/0c09380a1985438594dbdf334eafaa2b
work_keys_str_mv	AT tamaragoldfarb frequentandefficientuseofthesisterchromatidfordnadoublestrandbreakrepairduringbuddingyeastmeiosis AT michaellichten frequentandefficientuseofthesisterchromatidfordnadoublestrandbreakrepairduringbuddingyeastmeiosis
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Frequent and efficient use of the sister chromatid for DNA double-strand break repair during budding yeast meiosis.

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